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Bicd2 (NM_001039180) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210810
Bicd2 (Myc-DDK-tagged) - Mouse bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant 3
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $683.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bicd2
Synonyms 0610027D24Rik; 1110005D12Rik; AA408834; mKIAA0699
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210810 representing NM_001039180
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAGCTCAAAGAGGCATTTGGCCAGGCACATACAAACCACAAGAAGGTGGCTGCTGATGGTGAGA
GCCGGGAGGAGAGCCTGATCCAGGAGTCGGCCTCCAAGGAGCAGTACTACGTGCGGAAGGTGCTGGAGCT
GCAGACAGAGCTGAAACAGCTGCGCAACGTCCTCACCAACACCCAGTCTGAGAATGAGCGCCTCACGTCT
GTGGCCCAGGAGCTAAAAGAGATCAACCAGAATGTGGAGATCCAGCGTGGTCGCCTGCGGGATGACATCA
AGGAGTACAAGTTCCGGGAGGCCCGTCTACTTCAGGACTACTCAGAGCTGGAGGAGGAGAACATCAGCCT
GCAGAAACAAGTGTCTGTGCTCAGGCAGAACCAGGTGGAGTTTGAGGGCCTCAAGCATGAAATCAAGCGC
CTGGAGGAGGAGACAGAGTACCTCAACAGCCAGCTAGAGGATGCCATCCGGCTTAAGGAGATCTCTGAAC
GGCAGTTGGAGGAGGCGCTGGAGACACTGAAGACAGAGCGGGAGCAGAAGAACAACCTGCGCAAGGAGTT
GTCGCACTACATGAGCATCAACGATTCCTTCTATACCAGCCACCTGCAGGTCTCCTTAGATGGCCTCAAG
TTCAGTGATGATACTGTCACCGCAGAGCCCAACAATGACGCCGAAGCCCTGGTCAATGGCTTTGAGCACA
GTGGCTTGGTCAAATCATCCTTGGACAACAAGACATCCACACCCAGGAAGGATGGCCTAGCTCCACCCTC
CCCCAGCCTCGTCTCTGACCTGCTCAGTGAGCTCCACATATCTGAGATCCAGAAGCTGAAACAGCAGCTG
GTGCAGATGGAGCGGGAGAAGGTGGGCCTGCTGGCAACACTGCAGGACACACAGAAGCAGCTGGAGCAGG
CTCGGGGAACCCTCTCTGAGCAGCATGAGAAGGTGAATCGTCTCACAGAGAACCTTAGTGCCCTCCGGCG
CCTGCAGGCTGGCAAGGAGCGACAGACCTCGTTGGATAATGAGAAGGACCGTGACAGCCACGAAGACGGT
GACTACTATGAGGTGGACATCAATGGGCCTGAGATCCTGGCCTGCAAGTACCACGTGGCTGTGGCTGAGG
CTGGCGAGCTCCGGGAGCAGCTCAAGGCGTTGCGCAGCACACACGAAGCTCGCGAAGCCCAGCACGCAGA
AGAAAAGGGCCGGTATGAGGCTGAGGGCCAGGCACTCACTGAGAAGATCTCTCTGCTGGAGAAGGCTAGC
CACCAGGACCGAGAGCTGCTGGCCCATCTGGAAAAGGAGCTGAAGAAGGTGAGCGATGTGGCTGGTGAGA
CCCAGGGCAGCCTGAATGTGGCTCAGGATGAGCTGGTGACCTTCAGCGAGGAGCTGGCCAACCTCTACCA
CCATGTGTGCATGTGCAACAACGAGACGCCCAACCGTGTCATGCTCGACTATTATCGTGAGGGCCAGGGC
AAGGCTGGCCGCACCAGCCCAGAGGGCCGCGGGCGCCGGTCACCTGTCCTCTTGCCCAAGGGGCTGTTGG
CCACAGAGGTGGGCCGAGCAGATGGTGGGACTGGGGACAACAGTCCTTCACCCAGTTCTTCACTGCCGTC
ACCTCTGAGTGACCCCCGCCGGGAACCTATGAACATCTACAACCTGATCGCCATCATCCGAGACCAGATC
AAGCACCTGCAGGCAGCCGTGGACCGTACGACCGAGCTGTCCCGGCAGCGGATTGCCTCGCAGGAGCTGG
GCCCTGCTGTGGACAAGGACAAGGAAGCACTCATGGAGGAGATCCTCAAGCTGAAGTCCCTGCTGAGTAC
CAAGCGGGAGCAGATCACCACACTGCGCACCGTGCTCAAGGCCAACAAGCAGACAGCTGAGGTGGCCCTG
GCCAACCTGAAGAGCAAGTATGAGAACGAGAAGGCCATGGTGACCGAGACCATGATGAAGCTACGGAACG
AGCTCAAGGCCCTCAAGGAAGACGCAGCCACCTTCTCCTCCCTGCGTGCCATGTTTGCCACCAGGTGTGA
CGAATACATCACACAGCTGGATGAGATGCAGCGGCAGCTGGCAGCTGCCGAGGACGAGAAGAAGACCCTT
AACTCTCTGTTGCGCATGGCCATCCAGCAGAAGCTGGCGCTCACCCAGCGGCTGGAGCTGCTGGAGCTGG
ACCATGAACAGACCCGCAGGGGCCGCAGCAAGGCCGCCTCCAAGGCCAAGCCAGCCTCACCGAGCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210810 representing NM_001039180
Red=Cloning site Green=Tags(s)

MEQLKEAFGQAHTNHKKVAADGESREESLIQESASKEQYYVRKVLELQTELKQLRNVLTNTQSENERLTS
VAQELKEINQNVEIQRGRLRDDIKEYKFREARLLQDYSELEEENISLQKQVSVLRQNQVEFEGLKHEIKR
LEEETEYLNSQLEDAIRLKEISERQLEEALETLKTEREQKNNLRKELSHYMSINDSFYTSHLQVSLDGLK
FSDDTVTAEPNNDAEALVNGFEHSGLVKSSLDNKTSTPRKDGLAPPSPSLVSDLLSELHISEIQKLKQQL
VQMEREKVGLLATLQDTQKQLEQARGTLSEQHEKVNRLTENLSALRRLQAGKERQTSLDNEKDRDSHEDG
DYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKISLLEKAS
HQDRELLAHLEKELKKVSDVAGETQGSLNVAQDELVTFSEELANLYHHVCMCNNETPNRVMLDYYREGQG
KAGRTSPEGRGRRSPVLLPKGLLATEVGRADGGTGDNSPSPSSSLPSPLSDPRREPMNIYNLIAIIRDQI
KHLQAAVDRTTELSRQRIASQELGPAVDKDKEALMEEILKLKSLLSTKREQITTLRTVLKANKQTAEVAL
ANLKSKYENEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYITQLDEMQRQLAAAEDEKKTL
NSLLRMAIQQKLALTQRLELLELDHEQTRRGRSKAASKAKPASPSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039180
ORF Size 2238 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039180.2, NP_001034269.1
RefSeq Size 4555 bp
RefSeq ORF 2241 bp
Locus ID 76895
UniProt ID Q921C5
Cytogenetics 13 A5
MW 84.7 kDa
Summary Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates and stabilizes the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:11483508, PubMed:25035494, PubMed:24986880, PubMed:22956769). Facilitates the binding of RAB6A to the Golgi by stabilizing its GTP-bound form (PubMed:25962623). Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport via its interaction with RAB6A and recruitment of the dynein-dynactin motor complex (PubMed:12447383, PubMed:25962623). Contributes to nuclear and centrosomal positioning prior to mitotic entry through regulation of both dynein and kinesin-1. During G2 phase of the cell cycle, associates with RANBP2 at the nuclear pores and recruits dynein and dynactin to the nuclear envelope to ensure proper positioning of the nucleus relative to centrosomes prior to the onset of mitosis (PubMed:20386726).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001039180" in other vectors (2)
SKU Description Size Price
MR210810L3 Lenti ORF clone of Bicd2 (Myc-DDK-tagged) - Mouse bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant 3 10 ug
$983.00
MR210810L4 Lenti ORF clone of Bicd2 (mGFP-tagged) - Mouse bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant 3 10 ug
$983.00

Plasmid Map for MR210810

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210810
Circular map for MR210810

file-alt Citations

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