Rnf10 (NM_016698) Mouse Tagged ORF Clone
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Rnf10 |
| Synonyms | AA675014; mKIAA0262; RIE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016698 |
| ORF Size | 2412 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016698.2, NP_057907.2 |
| RefSeq Size | 3121 bp |
| RefSeq ORF | 2415 bp |
| Locus ID | 50849 |
| UniProt ID | Q3UIW5 |
| Cytogenetics | 5 56.01 cM |
| MW | 88.3 kDa |
| Summary | The protein encoding this gene is a member of the really interesting new gene finger protein family. Members of this family contain protein motifs similar to zinc finger domains and are involved in many processes that include transcriptional regulation, DNA repair and signal transduction. Expression of this gene is upregulated during neuronal differentiation of cultured cells, and inhibition of its expression impairs differentiation and cell cycle exit, providing evidence for a function in neuronal differentiation. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016698" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC200724 | Rnf10 (untagged) - Mouse ring finger protein 10 (Rnf10), (10ug) | 10 ug |
$737.00
|
|
| MR210752L3 | Lenti ORF clone of Rnf10 (Myc-DDK-tagged) - Mouse ring finger protein 10 (Rnf10) | 10 ug |
$1,036.00
|
|
| MR210752L4 | Lenti ORF clone of Rnf10 (mGFP-tagged) - Mouse ring finger protein 10 (Rnf10) | 10 ug |
$1,036.00
|
|
Citations
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