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Nop2 (NM_138747) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210695
Nop2 (Myc-DDK-tagged) - Mouse NOP2 nucleolar protein homolog (yeast) (Nop2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $727.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nop2
Synonyms 120kDa; A530002O17; Nol1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210695 representing NM_138747
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGCAAGTTGGATCCTACGAAGAAGGAGAAGCGTGGACCCGGCCGAAAGGCCCGAAAACAGAAGG
GTGCGGAGACCGAATTGGTCAGATTTTTGCCTGCAGCTGGCGATGAGAATTCCAAGAGGCTGTCGAGTCG
TGCCCGAAAGCGGGCAGCCAAGAGGAGGGCAGGGTCTGTTGACGTCCCTAAGCCAAATAAGTCTCCTGGG
ATCAAAACATTGCCTGGAGAGTTATCCAAAGGAGCAGTCCAGGCTCGAGGTAAGAAGCGCCCAGCACCAA
TTCAAAACAGTGATGGGGACGAGGAGGAAGACTCGGGGGAAGACGATGTGGTGACCCAGGGGGACCTTTG
GGGCTCTGAGGACAGTGATGAAGATATGGTGGATGACTATGGAGCTGCCTCTAACTCGGAGGATGAGGAG
GAAAAGCTGTTGCCTATTGAAAGAGCTGCCCTGAAGCAGAAAGCCCAGGATGCCACAGCTGGGGTCCTGT
GGAATGAAGAGGACACTGATGAAGATGAAGATGATGATGGTGTGTCCCCTGAGTCCCACCCCAGAAAGGA
TGACAAAGCAGAGGGGGATTTGCAGATTAATGTGGAAGATGAGGAAGCCTTTGTGCTGCCCCCTGCTGGG
GAGACGGACCAGGATGGCCAGGCTCCAGACCTGCAGCGAGTTCACAAGCGGATCCAGGACATCGTGGGAG
TGCTGCGGGATTTCGGAGCTCAGCGAGAAGAGGGCCGGTCTCGAGCTGAGTATCTAAGTCGGCTTCAGAA
GGACCTGGCCACTTACTATTCTTATGGAGACTTCCTGCTGAGCAAGCTCATGGAGCTGTTCCCTCTGTCT
GAGTTGATCGAGTTCTTAGAAGCTAATGAGGTGCCCCGGCCAATCACCCTTCGGACCAACACCTTGAAAA
CCCGTCGCCGAGACCTTGCTCAGGCTCTGATCAATCGTGGGGTTAATCTGGATCCACTGGGGAAGTGGTC
AAAGTCTGGACTTGTGGTATATGATTCTTCAGTGCCTATTGGCGCTACCCCTGAGTACCTCGCTGGACAC
TATATGCTGCAGGGAGCTTCCAGTATGTTGCCCGTCATGGCCCTGGCACCTCAGGAGCATGAGCGGATCT
TAGACATGTGCTGTGCTCCTGGGGGGAAGACCAGCTACATAGCTCAGCTGATGAAGAACACAGGAGTGAT
CCTTGCCAACGATGCCAATGCAGACCGGCTCAAGAGCGTGGTGGGCAACCTGCACCGCTTGGGAGTCACC
AACACCATCATCAGCCACTATGATGGGCGCCAGTTCCCCAAGGTGGTAGGGGGCTTTGATCGAGTGCTAC
TGGATGCTCCTTGTAGTGGCACAGGAGTCATCTCCAAAGACCCTGCTGTGAAGACGAACAAGGATGAGAA
AGACATCCAGCGCTGTGCCCACCTTCAGAAGGAACTGCTGCTCAGTGCTATCGACTCAGTGAACGCTGCC
TCCAAGACCGGAGGCTACCTGGTCTACTGCACCTGTTCCATTACGGTGGAAGAGAACGAGTGGGTGGTAG
ACTATGCCTTGAAGAAGAGGAATGTGCGGCTGGTGCCCACCGGCCTGGACTTCGGCCAGGAAGGTTTTAC
CCGCTTCCAAGCAAGGCGCTTTCACCCCACTCTGCGTTCCACCCGGCGCTTCTACCCTCACACTCACAAT
ATGGACGGTTTCTTTATCGCCAAGTTCAAGAAATTTTCCAATTCTATTCCCCAGCCCCATGCAGGAAACT
CGGCAGCAGCTACCCCTACAGAGCCTGACCTGAAGGATCAGGTCACCCCAAAGTCTGAGAACGGTAGTCA
GCCCACCAAGAAAGCCCGTGGGGCTGTGAAGGCAAAGCAGCAGTTGCTGAGACAGCCACATTCCAAGAAG
CCCTTCCAGAAGTTGAACGGCATCGCCAAAGGGCCAGGCTTGTCCACTGAACCTTCTGTCCCAGATGCCC
AAGTGTCTACCAGGCCCAGTCAGTCTGCTGGGAATGCTGACGTGAACAGCAAACGAAAGAGGTCTGAGAA
GCTGAAGCAGCGGGGACCTAAGTGGAAGCCCTCTAAGGAGGCTGCTGTCCCAAAGCCGAGCGCCCCTTCC
AGGGTTGAGGACTCGAGAACACCTCCTGTGCCGACTCCCTCTGAAATCCGTGCTGCCCCAAGGCCTAAGG
ACTGTGCTCCATCCCTTGGGGAAGCCAAAAAGAAACAGAAAGGAAAGCAGCAGTTAGCACAGCAGCCTGC
TAATGGAGCTGCTCCCCTGAAAGAAGATGCTGTTTCCAAGGGACCCTCAGCCCCCTTTGTGTCTCCCCAC
AGTTCCACCAGGCCCCCACCAGCAAAAAGGAGGAAATCAATGACAAAGGGCAACAGCCAGCCTCTGTTGT
CT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210695 representing NM_138747
Red=Cloning site Green=Tags(s)

MGRKLDPTKKEKRGPGRKARKQKGAETELVRFLPAAGDENSKRLSSRARKRAAKRRAGSVDVPKPNKSPG
IKTLPGELSKGAVQARGKKRPAPIQNSDGDEEEDSGEDDVVTQGDLWGSEDSDEDMVDDYGAASNSEDEE
EKLLPIERAALKQKAQDATAGVLWNEEDTDEDEDDDGVSPESHPRKDDKAEGDLQINVEDEEAFVLPPAG
ETDQDGQAPDLQRVHKRIQDIVGVLRDFGAQREEGRSRAEYLSRLQKDLATYYSYGDFLLSKLMELFPLS
ELIEFLEANEVPRPITLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKSGLVVYDSSVPIGATPEYLAGH
YMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYIAQLMKNTGVILANDANADRLKSVVGNLHRLGVT
NTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDIQRCAHLQKELLLSAIDSVNAA
SKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFQARRFHPTLRSTRRFYPHTHN
MDGFFIAKFKKFSNSIPQPHAGNSAAATPTEPDLKDQVTPKSENGSQPTKKARGAVKAKQQLLRQPHSKK
PFQKLNGIAKGPGLSTEPSVPDAQVSTRPSQSAGNADVNSKRKRSEKLKQRGPKWKPSKEAAVPKPSAPS
RVEDSRTPPVPTPSEIRAAPRPKDCAPSLGEAKKKQKGKQQLAQQPANGAAPLKEDAVSKGPSAPFVSPH
SSTRPPPAKRRKSMTKGNSQPLLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138747
ORF Size 2382 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138747.2, NP_620086.2
RefSeq Size 2626 bp
RefSeq ORF 2385 bp
Locus ID 110109
Cytogenetics 6 59.32 cM
MW 87.4 kDa
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Documents
Resources
"NM_138747" in other vectors (4)
SKU Description Size Price
MC205642 Nop2 (untagged) - Mouse NOP2 nucleolar protein homolog (yeast) (Nop2), (10ug) 10 ug
$727.00
MG210695 Nop2 (tGFP-tagged) - Mouse nucleolar protein 1 (Nol1) 10 ug
$927.00
MR210695L3 Lenti ORF clone of Nop2 (Myc-DDK-tagged) - Mouse NOP2 nucleolar protein homolog (yeast) (Nop2) 10 ug
$1,027.00
MR210695L4 Lenti ORF clone of Nop2 (mGFP-tagged) - Mouse NOP2 nucleolar protein homolog (yeast) (Nop2) 10 ug
$1,027.00

Plasmid Map for MR210695

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210695
Circular map for MR210695

file-alt Citations

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