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Pcdhb15 (NM_053140) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210652
Pcdhb15 (Myc-DDK-tagged) - Mouse protocadherin beta 15 (Pcdhb15)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $720.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcdhb15
Synonyms Pcdhb7; PcdhbO
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210652 representing NM_053140
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCAGATTGGAACAAGCTGTGCAGAAAAGGCAAGTACTGTTTCTTTATGTGTTTCTTGGAGCGT
CTTGGGCTAGCGCCGAACAGCTTCAGTATTCTGTGGCAGAGGAAACCGAGAGAGGGACTTTCCTAGCCAA
TCTAGCAAAGGATTTGGGGCTGGGGCTGGGGGAACTGTCAGCCCGGGAAGCTAGAATTGTTTCAGACCAG
AACACACGATTTTTACTGCTCAGTCCGCTTACTGGAGAATTAATTCTAAATGAGAAGCTGGACCGAGAGG
AACTGTGCGGCCCCACAGAGCCCTGTGTGCTGACTTTCCAGTTGCTGTTGGAAAGACCATTTCAGATTTA
TCGCGCTGCACTACATATCAAAGATATAAATGATAATTCTCCAGTATTCCTAGACAAAGAAATACTCTTG
AAAATATCAGAAAGTACCACTCCAGGGGCGACGCTTCTCTTGGAAAGGGCACAGGATGCCGATGTGGGAA
CGAACAGCCTGAGTAACTACACCATCAGCCCCAATGATTATTTCCATATTCACGTGCATGATGGAGGGGA
GGGGCCTATCATTCCTGAACTGGTACTGGATAAAATGCTGGACCGGGAAGAAATACCAGAATTCACTTTA
ACTCTCACAGCCTTAGATGGTGGCTCTCCACCCAGATCTGGGACAGCCTTGGTGCAAATCTTGGTGTTGG
ACATAAATGACAACTCCCCTCAATTCGTGCAGTCACTTTACAAAGTTCAGTCACCTGAAAACACCCCTGT
TGGCTCCTTGGTTGTCGCTGTGTCTGCCAGGGACTTAGACACAGGAAGTTACGGACAAATAGACTACACA
TTTTTTTATGCCACAGAAAGAATTCTCCAAACGTTTCAAATCAATTCTACATCTGGCGAACTTTATCTTA
AACGCGAACTCAACTATGAAGCGATTCAAACTTATTCGATAACTATTCAGGCCAGAGATGGTGGTGGACT
TTCTGGGAAATGTGCTGTGGTTGTAGAGGTAATGGACGTGAATGATAACCCACCTGAATTTCTCTTGTCG
TCACTTAATAGCCCAATTCCAGAAAATTCACAAGAGACAGTTGTTGCTGTATTTAAAATTAGAGACAGAG
ATTCGGGGAACAATGGAAAAACCCTGTGCTCCATTGCGAATGATCTCCCTTTCGTCCTGAAGCCATCTGT
GGAAAATTTCTATACTCTGGTAACAGAGAAGCCTTTAGATAGAGAATCAAACACTGAATACAACATCACC
ATCACAGTCACCGACATGGGCATACCCAGGCTCACAACCCAGCACACCATAACAGTGCAGGTCTCTGACA
TCAACGACAACGCCCCCACCTTCACCCAAACCTCCTACACCATGTTCGTCCGCGAGAACAACAGCCCCGC
CCTGCACATTGGCACCATCAGCGCCACAGACTCAGACTCAGGCTCCAATGCCCACATCACCTACTCGTTG
CGGCTGCCTCACGACCCGCAGCTGGCCCTGGACTCGCTCATCTCCATCAATGCAGACAATGGGCAACTGT
TCGCGCTCAGGGCTCTGGACTATGAGGCCCTGCAGGCCTTCGAGTTCCACGTGGGTGCAATAGACCAAGG
CTCCCCTGCGCTCAGCAGTCAGGCTCTGGTGCGCGTGGTGATACTGGACGACAATGACAATGCGCCCTTC
GTGCTCTACCCGATGCAGAACGCCTCTGCACCCTACACTGAGCTGCTGCCCAGGGCGGCAGAACCCGGAT
ACCTGGTCACCAAGGTGGTGGCAGTTGACCGTGATTCTGGCCAGAACGCCTGGCTGTCATTTCAGTTGCT
TAAGGCCACAGAGCCCGGACTGTTTAGTGTGTGGGCGCACAATGGTGAGGTGCGCACCACCAGGCTACTA
AGCGAGCGAGATGTGCCCAAGCATAAGCTGCTAGTGGTGGTCAAGGATAATGGAGAGCCTCCACGCTCTG
CCAGTGTCACACTGCATGTGTTGCTGGTCGATGGCTTCTCTCAGCCCTTCCTGATTCTGCCAGAGGTGGC
GCGCGACCCTGCACAAGAAGATGATGAGCTAACACTCTACCTGGTCATTGCCTTGGCTTCTGTGTCTTCT
CTCTTCCTGGTATCTGTGCTGCTGTTCGTTGGAGTAAGACTCTGCAGGAGGGCCCGGGCAGCCTCTCCGG
GTGTCTGCTTTGTGCCTGAAGAACACTTTCCTGGCCACCTGGTGGATGTCAGCGGTGCAGGGACCCTGTC
CCAGAACTACCAGTATGAGGTGTGTCTGACTGGAGGATCAGGGATAACCAGTGAATTTAAGTTTCTAAGT
CCAATTGCCTCTAACTTCCTAACCGAAAGCACAGGGAGAGAAATAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210652 representing NM_053140
Red=Cloning site Green=Tags(s)

MEARLEQAVQKRQVLFLYVFLGASWASAEQLQYSVAEETERGTFLANLAKDLGLGLGELSAREARIVSDQ
NTRFLLLSPLTGELILNEKLDREELCGPTEPCVLTFQLLLERPFQIYRAALHIKDINDNSPVFLDKEILL
KISESTTPGATLLLERAQDADVGTNSLSNYTISPNDYFHIHVHDGGEGPIIPELVLDKMLDREEIPEFTL
TLTALDGGSPPRSGTALVQILVLDINDNSPQFVQSLYKVQSPENTPVGSLVVAVSARDLDTGSYGQIDYT
FFYATERILQTFQINSTSGELYLKRELNYEAIQTYSITIQARDGGGLSGKCAVVVEVMDVNDNPPEFLLS
SLNSPIPENSQETVVAVFKIRDRDSGNNGKTLCSIANDLPFVLKPSVENFYTLVTEKPLDRESNTEYNIT
ITVTDMGIPRLTTQHTITVQVSDINDNAPTFTQTSYTMFVRENNSPALHIGTISATDSDSGSNAHITYSL
RLPHDPQLALDSLISINADNGQLFALRALDYEALQAFEFHVGAIDQGSPALSSQALVRVVILDDNDNAPF
VLYPMQNASAPYTELLPRAAEPGYLVTKVVAVDRDSGQNAWLSFQLLKATEPGLFSVWAHNGEVRTTRLL
SERDVPKHKLLVVVKDNGEPPRSASVTLHVLLVDGFSQPFLILPEVARDPAQEDDELTLYLVIALASVSS
LFLVSVLLFVGVRLCRRARAASPGVCFVPEEHFPGHLVDVSGAGTLSQNYQYEVCLTGGSGITSEFKFLS
PIASNFLTESTGREIE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053140
ORF Size 2358 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053140.3, NP_444370.1
RefSeq Size 2795 bp
RefSeq ORF 2361 bp
Locus ID 93886
Cytogenetics 18 B3
MW 86.7 kDa
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"NM_053140" in other vectors (4)
SKU Description Size Price
MC201932 Pcdhb15 (untagged) - Mouse protocadherin beta 15 (Pcdhb15), (10ug) 10 ug
$721.00
MG210652 Pcdhb15 (tGFP-tagged) - Mouse protocadherin beta 15 (Pcdhb15) 10 ug
$920.00
MR210652L3 Lenti ORF clone of Pcdhb15 (Myc-DDK-tagged) - Mouse protocadherin beta 15 (Pcdhb15) 10 ug
$1,020.00
MR210652L4 Lenti ORF clone of Pcdhb15 (mGFP-tagged) - Mouse protocadherin beta 15 (Pcdhb15) 10 ug
$1,020.00

Plasmid Map for MR210652

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210652
Circular map for MR210652

file-alt Citations

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