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Rtn1 (NM_153457) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210617
Rtn1 (Myc-DDK-tagged) - Mouse reticulon 1 (Rtn1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $715.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rtn1
Synonyms Nsp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210617 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCACCGCCGGATCTGCAAGATGAGCCGCTGTCTCTGGGTAGCCCCGGGTCCCAGTGGTTCGGGG
GCCGGGGGGATGGGGAAGACGAAGCGACGGCGGTGATGGGGGCCAGGCCAGCGCAGCAGGATGGGGAGCC
CGCCTGGGGGTCGGGCGCTGGAGCTGGGGTAACGTCGTCGCGGGAACTGTGCTCCGGCCCCGCCCGGTCG
CCTCCCGTCGCCATGGAAACTGCATCCACAGGTATGGCAGCTGTTCCCGATGCCCTCGACCACTCTCCCT
CTTCAACACTAAAAGATGGGGAAGGGGCATGTTACACATCCCTGATTTCTGACGTCTGCTATCCACCTCG
GGAGGACTCTGCATACTTTACTGGAATTCTTCAGAAGGAAAATGGTCACATCACCACTTCCGAGAGCCCT
GAGGAGCCGGAGACCCCCGGACCCTCGTTACCAGAAGTGCCCGGGATGGAGCCACAGGGCTTATTGAGTT
CTGATTCTGGAATAGAGATGACTCCTGCTGAGTCCACTGAGGTGAACAAGATCCTAGCTGACCCCCTAGA
CCAGATGAAGGCAGAAGCCTACAAATACATTGACATAACCAGGCCACAGGAGGCAAAAGGCCAGGAGGAG
CAACACCCTGGGCTAGAAGATAAAGACTTGGACTTTAAAGACAAGGACACTGAAGTCTCCACAAAAGCAG
AAGGGGTTCGAGCACCAAACCAGCCAGCTCCTGTGGAGGGGAAACTCATCAAGGACCACTTATTCGAAGA
ATCTACCTTTGCTCCTTACATAGATGAGCTCTCTGATGAGCAGCACAGGGTGTCTCTGGTCACTGCCCCT
GTCAAGATCACACTGACGGAGATCGAACCTCCTCTTATGACTGCCACACAAGAGACAATCCCCGAGAAGC
AAGACCTGTGTCTGAAGCCAAGTCCTGACACAGTGCCCACTGTCACTGTCTCAGAGCCTGAAGATGACAG
CCCAGGATCTGTCACGCCTCCATCTTCTGGAACAGAACCATCTGCTGCAGAGTCCCAGGGGAAAGGCAGC
GTCTCCGAGGATGAGTTGATTGCTGCCATTAAAGAAGCAAAGGGGTTATCATATGAAACCACCGAGAGTC
CAAGGCCAGTGGGCCAGGTGGCCGACAAACCCAAAACCAAGACCAGGTCTGGGCTGCCCACCATCCCCAG
TCCCCTCGACCAGGAGGCCAGCAGTGCCGAGTCCGGAGACTCAGAAATTGAGCTGGTGTCAGAGGACCCC
ATGGCCTCTGAGGATGCCCTGCCCTCTGGCTATGTGAGCTTCGGTCATGTGTCGGGCCCGCCCCCCTCTC
CTGCCTCTCCATCCATCCAGTACAGCATCCTGCGGGAGGAGCGTGAGGCCGAGCTGGACAGCGAGCTCAT
CATCGAGTCCTGTGATGCCTCCTCAGCCTCGGAGGAGAGCCCCAAGCGCGAGCAGGACTCGCCCCCGATG
AAACCGGGTGCCCTGGACGCCATCCGCGAGGAGACAGGCTCTCGGGCCACCGAAGAACGAGCGCCCAGCC
ACCAAGGCCCTGTGGAGCCCGACCCGATGCTCAGCTTTGCCCCCGCTGCCGCCCTCCAATCCCGCCCAGA
GCCATCGTCTGGAGATGGAGCCTCGGTCCCGGAGCCGCCCAGATCACAGCAGCAGAAGCCTGAAGAGGAA
GCTGTGAGTTCCAGTCAAAGTCCCACGGCCACCGAAATTCCTGGACCACTTGGTTCTGGCCTCATGCCCC
CTCTGCCTTTCTTCAACAAGCAAAAAGCTATTGACCTTCTGTACTGGCGGGACATCAAGCAGACTGGGAT
TGTGTTCGGGAGCTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCAGCGTTGTGAGCGTCGTTGCCTAC
CTGGCCCTGGCCGCCCTCTCTGCCACCATCAGCTTCCGCATCTACAAGTCCGTTCTACAAGCTGTGCAGA
AAACAGATGAGGGTCACCCTTTCAAGGCCTACCTGGAGCTGGAGATCACCCTGTCCCAGGAGCAGATCCA
GAAGTACACAGACTGCCTGCAGCTGTATGTGAACAGCACTCTGAAGGAGCTACGGAGGCTTTTCCTGGTC
CAGGACCTGGTGGATTCCTTAAAATTTGCAGTCCTCATGTGGCTCCTGACCTACGTTGGCGCGCTCTTCA
ATGGCCTGACCCTGCTGCTTATGGCTGTGGTTTCGATGTTTACTCTACCTGTTGTGTACGTTAAGCACCA
GGCACAAGTTGACCAATATCTGGGACTTGTGAGGACTCACATAAACACCGTCGTGGCAAAGATCCAGGCT
AAAATCCCCGGCGCCAAGAGGCACGCTGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210617 protein sequence
Red=Cloning site Green=Tags(s)

MAAPPDLQDEPLSLGSPGSQWFGGRGDGEDEATAVMGARPAQQDGEPAWGSGAGAGVTSSRELCSGPARS
PPVAMETASTGMAAVPDALDHSPSSTLKDGEGACYTSLISDVCYPPREDSAYFTGILQKENGHITTSESP
EEPETPGPSLPEVPGMEPQGLLSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPQEAKGQEE
QHPGLEDKDLDFKDKDTEVSTKAEGVRAPNQPAPVEGKLIKDHLFEESTFAPYIDELSDEQHRVSLVTAP
VKITLTEIEPPLMTATQETIPEKQDLCLKPSPDTVPTVTVSEPEDDSPGSVTPPSSGTEPSAAESQGKGS
VSEDELIAAIKEAKGLSYETTESPRPVGQVADKPKTKTRSGLPTIPSPLDQEASSAESGDSEIELVSEDP
MASEDALPSGYVSFGHVSGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPM
KPGALDAIREETGSRATEERAPSHQGPVEPDPMLSFAPAAALQSRPEPSSGDGASVPEPPRSQQQKPEEE
AVSSSQSPTATEIPGPLGSGLMPPLPFFNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAY
LALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLV
QDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQA
KIPGAKRHAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153457
ORF Size 2340 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153457.7
RefSeq Size 3632 bp
RefSeq ORF 2343 bp
Locus ID 104001
UniProt ID Q8K0T0
Cytogenetics 12 C3
MW 83.6 kDa
Summary May be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells.UniProtKB/Swiss-Prot Function
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"NM_153457" in other vectors (4)
SKU Description Size Price
MC203362 Rtn1 (untagged) - Mouse reticulon 1 (Rtn1), transcript variant 1, (10ug) 10 ug
$715.00
MG210617 Rtn1 (tGFP-tagged) - Mouse reticulon 1 (Rtn1), transcript variant 1 10 ug
$915.00
MR210617L3 Lenti ORF clone of Rtn1 (Myc-DDK-tagged) - Mouse reticulon 1 (Rtn1), transcript variant 1 10 ug
$1,015.00
MR210617L4 Lenti ORF clone of Rtn1 (mGFP-tagged) - Mouse reticulon 1 (Rtn1), transcript variant 1 10 ug
$1,015.00

Plasmid Map for MR210617

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210617
Circular map for MR210617

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