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Cul5 (NM_027807) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210611
Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $783.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cul5
Synonyms 4921514I20Rik; 8430423K24Rik; AI852817; C030032G03Rik; C330021I08Rik; VACM-1; VACM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210611 representing NM_027807
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTCTCTCGCGTGGGCAGGCCGGGGTGACCATGTAGCTGGAAAGCCCGAGGAAGCACGGCTGCCCG
GGACGAGCTCGGCGCTGACGGCACGCCGCTCCGGCGTCCCCCGCATCCCCCGCCGCGGCCTGCGGGGTCC
TGCTGGGAACCCCGGCCTCTCGAGGAGGCCTGGCCCCGAGCGCCGCCAAGTCTCGCCCCGTCTCGCGAGA
GTCCAAGTTGAGAACATGGCGACGTCTAATCTGTTAAAGAATAAAGGTTCTCTCCAGTTTGAAGACAAGT
GGGACTTCATGCATCCAATTGTTTTGAAGCTTTTACGCCAGGAATCTGTAACAAAACAGCAGTGGTTTGA
TCTATTTTCGGATGTACATGCTGTCTGTCTCTGGGATGATAAAGGCTCATCAAAAATTCATCAGGCTTTA
AAAGAAGATATTCTTGAGTTTATTAAGCAAGCACAGGCTCGTGTACTGAGCCATCAAGATGACACAGCTT
TGCTGAAGGCATATATTGTTGAATGGCGGAAATTCTTCACACAGTGTGATATTTTACCAAAACCTTTTTG
TCAATTAGAGGTGACTCTATTGGGTAAACAAAGCAGCAATAAAAAATCAAATATGGAAGACAGTATTGTT
CGAAAGCTCATGCTTGATACGTGGAATGAGTCGATTTTTTCAAATATAAAGAACAGACTCCAGGACAGTG
CAATGAAGCTGGTGCATGCTGAGAGATTAGGGGAAGCTTTTGATTCCCAGCTGGTCATCGGGGTGCGAGA
GTCCTATGTTAATCTTTGCTCCAACCCCGAGGACAAGCTTCAGATCTATAGGGATAATTTTGAGAAGGCA
TACTTGGACTCAACAGAGAGGTTTTATAGAACACAGGCACCCTCATATTTACAGCAAAATGGTGTGCAGA
ATTACATGAAATATGCAGATGCTAAATTAAAAGAAGAAGAAAAAAGAGCACTCCGATACTTAGAAACACG
ACGAGAGTGTAACTCTGTGGAAGCACTCATGGAATGCTGTGTAAATGCGCTGGTGACCTCCTTTAAAGAG
ACTATTTTAGCAGAATGCCAAGGCATGATCAAGCGAAATGAAACTGAAAAGTTACATTTGATGTTTTCCT
TGATGGACAAAGTTCCTAATGGGATAGAGCCGATGTTGAAGGACTTGGAGGAGCATATTATAAGTGCGGG
CCTAGCAGACATGGTGGCCGCAGCTGAAACCATCACTACTGACTCTGAGAAGTATGTGGAGCAATTACTT
ACACTGTTTAATAGATTCAGTAAACTGGTCAAAGAAGCTTTTCAGGATGATCCTCGTTTCCTTACTGCAA
GAGATAAGGCATATAAAGCAGTTGTTAATGATGCTACTATATTTAAACTTGAATTGCCTTTGAAGCAAAA
AGGAGTGGGGTTGAAAACTCAGCCTGAGTCAAAATGCCCCGAGTTGCTTGCCAATTACTGTGACATGTTG
TTAAGGAAAACGCCATTAAGCAAAAAACTAACATCTGAGGAGATTGAAGCAAAGCTTAAAGAAGTGCTCT
TGGTACTTAAATATGTACAAAACAAAGATGTTTTTATGAGGTATCACAAAGCTCATCTTACCCGACGTCT
CATATTGGACATCTCTGCTGATAGTGAGATTGAAGAAAACATGGTAGAGTGGCTAAGAGAAGTTGGTATG
CCAGCAGATTATGTGAACAAGCTTGCTAGAATGTTTCAGGACATAAAAGTATCTGAAGACTTGAACCAAG
CTTTTAAGGAAATGCACAAAAATAATAAGTTGGCATTACCAGCTGATTCCGTTAATATAAAGATTTTGAA
TGCTGGTGCTTGGTCTAGAAGCTCCGAGAAAGTCTTTGTCTCACTTCCTACTGAACTGGAGGATTTGATA
CCTGAAGTAGAAGAATTTTACAAAAAAAATCACAGTGGTAGAAAATTACACTGGCACCATCTCATGTCAA
ATGGAATTATAACATTTAAAAATGAAGTAGGTCAGTATGATTTGGAAGTAACCACGTTTCAGTTGGCTGT
GTTGTTTGCATGGAACCAAAGGCCTAGAGAGAAAATCAGCTTTGAAAATCTAAAACTTGCAACGGAACTC
CCAGATGCTGAACTTAGAAGGACTTTATGGTCTTTAGTAGCTTTTCCCAAGCTCAAACGGCAAGTTTTGT
TGTATGACCCTCAAGTCAACTCACCCAAAGATTTTACAGAAGGCACCCTCTTCTCAGTGAACCAGGACTT
CAGTCTCATAAAAAATGCAAAAGTACAGAAAAGGGGGAAAATCAATTTGATTGGACGCTTGCAGCTCACT
ACAGAACGAATGAGAGAAGAAGAAAATGAAGGGATAGTCCAACTAAGAATATTAAGAACCCAGGAAGCCA
TCATACAAATAATGAAAATGAGAAAGAAAATTAGCAATGCCCAGCTGCAGACTGAATTAGTAGAAATTCT
GAAAAACATGTTCCTGCCTCAGAAGAAGATGATAAAGGAGCAGATGGAGTGGCTGATTGAACACAGGTAC
ATCCGGAGGGACGAGGCCGACATCAACACCTTCATCTACATGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210611 representing NM_027807
Red=Cloning site Green=Tags(s)

MRSLAWAGRGDHVAGKPEEARLPGTSSALTARRSGVPRIPRRGLRGPAGNPGLSRRPGPERRQVSPRLAR
VQVENMATSNLLKNKGSLQFEDKWDFMHPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGSSKIHQAL
KEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEVTLLGKQSSNKKSNMEDSIV
RKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKA
YLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKE
TILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLL
TLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDML
LRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGM
PADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLI
PEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATEL
PDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLT
TERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRY
IRRDEADINTFIYMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027807
ORF Size 2565 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027807.3, NP_082083.2
RefSeq Size 6023 bp
RefSeq ORF 2568 bp
Locus ID 75717
UniProt ID Q9D5V5
Cytogenetics 9 A5.3
MW 99.5 kDa
Summary Core component of multiple SCF-like ECS (Elongin BC-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027807" in other vectors (4)
SKU Description Size Price
MC222170 Cul5 (untagged) - Mouse cullin 5 (Cul5), transcript variant 1, (10ug) 10 ug
$862.00
MG210611 Cul5 (tGFP-tagged) - Mouse cullin 5 (Cul5) 10 ug
$983.00
MR210611L3 Lenti ORF clone of Cul5 (Myc-DDK-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 10 ug
$1,083.00
MR210611L4 Lenti ORF clone of Cul5 (mGFP-tagged) - Mouse cullin 5 (Cul5), transcript variant 1 10 ug
$1,083.00

Plasmid Map for MR210611

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210611
Circular map for MR210611

file-alt Citations

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