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Hcn3 (NM_008227) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210605
Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $713.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hcn3
Synonyms BCNG-4; Bcng4; Hac3; Hcn4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210605 representing NM_008227
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGAGGCGCGGCCGGCGGCGGGGGCCGGCGAAGCGGCGACCCCTGCACGCGAGACGCCTCCTG
CGGCTCCGGCCCAGGCCCGCGCGGCCTCAGGTGGGGTGCCGGAGTCTGCGCCCGAGCCGAAGAGGCGGCA
GCTCGGGACGCTGCTGCAGCCGACGGTCAACAAGTTCTCTCTCCGGGTCTTCGGCAGCCACAAAGCAGTA
GAAATCGAGCAGGAGAGGGTGAAGTCCGCCGGGGCCTGGATCATCCACCCCTACAGCGACTTCCGGTTTT
ACTGGGATCTCATCATGCTGCTGCTGATGGTGGGGAACCTCATAGTTCTGCCTGTGGGTATCACTTTCTT
CAAGGAGGAGAACTCTCCACCCTGGATCGTCTTCAATGTCCTCTCTGACACTTTCTTCCTGCTGGATCTG
GTGCTCAACTTCCGAACTGGCATCGTGGTGGAGGAAGGTGCCGAGATCCTGCTGGCGCCAAGGGCCATCC
GAACGCGTTACCTGCGCACCTGGTTCCTGGTTGATCTGATCTCCTCCATCCCTGTGGATTATATCTTCCT
AGTGGTGGAGCTGGAGCCACGACTAGATGCTGAGGTCTACAAAACGGCACGGGCCCTGCGCATCGTTAGA
TTCACCAAGATCCTTAGCCTGCTGCGGCTGCTCCGCCTCTCCCGCCTCATCCGCTACATACACCAGTGGG
AGGAGATCTTTCACATGACCTACGACCTGGCCAGTGCAGTGGTTCGCATCTTCAACCTCATTGGAATGAT
GTTGCTGCTGTGTCACTGGGACGGCTGTCTGCAGTTTCTGGTCCCTATGCTGCAGGACTTCCCGTCCGAC
TGCTGGGTCTCCATGAACCGCATGGTGAACCACTCGTGGGGCCGCCAGTATTCCCACGCCCTGTTCAAGG
CCATGAGTCACATGCTATGCATTGGCTATGGGCAGCAGGCACCGGTAGGCATGCCTGACGTCTGGCTCAC
CATGCTCAGTATGATTGTGGGCGCCACGTGTTATGCCATGTTCATCGGTCACGCCACCGCCCTCATCCAG
TCCCTGGACTCTTCCCGGCGACAGTACCAGGAGAAGTACAAGCAGGTGGAGCAGTACATGTCCTTCCACA
AGCTGCCCGCTGACACCCGGCAGCGCATCCACGAGTACTACGAGCATCGCTACCAGGGCAAGATGTTTGA
TGAAGAGAGCATCCTGGGGGAGCTGAGCGAGCCACTTCGGGAGGAGATTATTAACTTCACCTGCCGGGGC
CTGGTGGCCCACATGCCGCTGTTTGCTCATGCTGACCCCAGCTTCGTCACCGCAGTGCTCACCAAGCTCC
GTTTTGAGGTCTTCCAACCAGGGGACCTGGTGGTGCGTGAGGGCTCCGTGGGCAGGAAGATGTACTTCAT
CCAGCACGGGCTGCTGAGTGTGCTGGCACGTGGCGCCCGCGACACCCGCCTCACTGATGGATCCTACTTT
GGGGAGATCTGCCTGCTGACTCGAGGTCGGAGAACAGCCAGTGTAAGGGCTGACACCTATTGTCGCCTCT
ACTCGCTCAGCGTGGACCACTTCAATGCGGTGCTTGAGGAGTTCCCAATGATGCGCAGGGCTTTTGAGAC
GGTGGCCATGGACCGGCTTCGGCGCATCGGCAAAAAGAATTCGATACTGCAGCGGAAACGCTCTGAGCCG
AGTCCAGGCAGCAGCGGTGGCGTCATGGAGCAGCATTTGGTACAACACGACAGAGACATGGCTCGTGGTG
TTCGGGGCCTGGCTCCTGGTACAGGAGCTCGACTCAGTGGAAAGCCAGTGCTGTGGGAACCACTGGTGCA
CGCCCCTCTGCAGGCAGCTGCTGTGACCTCCAACGTGGCCATAGCCTTGACTCACCAGCGAGGCCCTCTG
CCCCTCTCCCCTGATTCTCCAGCCACCCTCCTAGCTCGATCTGCTAGACGCTCAGCAGGCTCCCCAGCCT
CCCCACTGGTGCCTGTCCGAGCAGGTCCTCTGCTGGCCCGGGGACCCTGGGCGTCCACTTCTCGCCTGCC
TGCTCCACCTGCCCGAACCCTCCATGCCAGCCTATCCCGGACAGGGCGTTCCCAGGTATCTCTGTTGGGC
CCTCCCCCAGGAGGAGGTGCTCGGAGGCTAGGACCTCGGGGCCGCCCACTTTCTGCCTCGCAACCCTCTC
TGCCTCAGCGAGCAACAGGGGATGGCTCTCCTAGGCGTAAAGGCTCTGGAAGTGAGCGCCTGCCCCCCTC
TGGGCTCTTGGCCAAACCTCCAGGGACAGTCCAGCCACCCAGGTCATCAGTGCCTGAGCCAGTTACCCCC
AGAGGTCCCCAAATTTCTGCCAACATG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210605 representing NM_008227
Red=Cloning site Green=Tags(s)

MEEEARPAAGAGEAATPARETPPAAPAQARAASGGVPESAPEPKRRQLGTLLQPTVNKFSLRVFGSHKAV
EIEQERVKSAGAWIIHPYSDFRFYWDLIMLLLMVGNLIVLPVGITFFKEENSPPWIVFNVLSDTFFLLDL
VLNFRTGIVVEEGAEILLAPRAIRTRYLRTWFLVDLISSIPVDYIFLVVELEPRLDAEVYKTARALRIVR
FTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSD
CWVSMNRMVNHSWGRQYSHALFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVGATCYAMFIGHATALIQ
SLDSSRRQYQEKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQGKMFDEESILGELSEPLREEIINFTCRG
LVAHMPLFAHADPSFVTAVLTKLRFEVFQPGDLVVREGSVGRKMYFIQHGLLSVLARGARDTRLTDGSYF
GEICLLTRGRRTASVRADTYCRLYSLSVDHFNAVLEEFPMMRRAFETVAMDRLRRIGKKNSILQRKRSEP
SPGSSGGVMEQHLVQHDRDMARGVRGLAPGTGARLSGKPVLWEPLVHAPLQAAAVTSNVAIALTHQRGPL
PLSPDSPATLLARSARRSAGSPASPLVPVRAGPLLARGPWASTSRLPAPPARTLHASLSRTGRSQVSLLG
PPPGGGARRLGPRGRPLSASQPSLPQRATGDGSPRRKGSGSERLPPSGLLAKPPGTVQPPRSSVPEPVTP
RGPQISANM

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_008227
ORF Size 2337 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008227.1, NP_032253.1
RefSeq Size 3268 bp
RefSeq ORF 2340 bp
Locus ID 15168
UniProt ID O88705
Cytogenetics 3 F1
MW 87.1 kDa
Summary Hyperpolarization-activated potassium channel. May also facilitate the permeation of sodium ions.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008227" in other vectors (4)
SKU Description Size Price
MC205963 Hcn3 (untagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3), (10ug) 10 ug
$714.00
MG210605 Hcn3 (tGFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3) 10 ug
$789.00 $913.00
MR210605L3 Lenti ORF clone of Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3) 10 ug
$1,013.00
MR210605L4 Lenti ORF clone of Hcn3 (mGFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3) 10 ug
$1,013.00

Plasmid Map for MR210605

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210605
Circular map for MR210605

file-alt Citations

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