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Ccnf (NM_007634) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210593
Ccnf (Myc-DDK-tagged) - Mouse cyclin F (Ccnf)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $711.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccnf
Synonyms CycF; Fbxo1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210593 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGCGGCGGTGTGATCCATTGTAGGTGTGCCAAGTGTTTCTGTTATCCTACTAAACGAAGAATCA
AAAGAAGACCCAGAAACTTAACCATCTTGAGTCTCCCAGAAGATGTACTCTTTCATATCCTGAAATGGCT
TTCTGTTGGGGACATCCTTGCTGTCCGAGCTGTCCACTCCCACCTCAAGTACCTGGTGGACAACCATGCC
AGTGTGTGGGCATCCGCCAGCTTCCAGGAGCTGTGGCCTTCTCCACAGAACCTGAAGCTCTTTGAAAGGG
CTGCTGAAAAGGGAAATTTTGAAGCTGCTGTGAAGTTGGGGATTGCCTACCTCTACAATGAAGGCCTGTC
TGTGTCAGATGAGGCCTGCGCAGAAGTGAACGGCTTGAAGGCCTCTCGCTTCTTCAGCATGGCTGAGAGA
CTGAATACGGGTTCTGAGCCCTTCATTTGGCTCTTCATCCGCCCACCGTGGTCAGTGTCCGGAAGCTGCT
GCAAGGCTGTGGTTCATGACAGCCTCCGAGCGGAGTGTCAGTTACAGAGAAGTCATAAAGCTTCCATACT
ACACTGCTTGGGAAGGGTGCTAAATCTTTTTGAGGACGAAGAGAAAAGGAAGCAGGCGCGTAGCCTTTTG
GAAGAGTCTTCTCGTCAGGGATGCTTAATCAGCTCATACCTCCTTTGGGAAAGCGACAGAAAGATGTCCG
ACCCCGGACGATGCCTCCACAGCTTCCGGAAACTCAGGGACTACGCTGCGAAAGGCTGCTGGGAAGCACA
GCTGGCTTTGGCCAAAGCCTGTGCAGGTGGAAGCCAACTTGGACTGGAAGGGAAGGCCTGCAGTGAATCG
GTCTGCCAGCTCTTCCAGGCCTCCCAGGCTGTCAACAAGCAGCAGATATTCTCTGTGCAGAAGGGGCTCA
GCGACACCATGAGGTACATTCTAATCGACTGGTTGGTGGAAGTTGCCACAATGAAGGACTTTACAAGCCT
GTGTCTTCACCTGACTGTGGAGTGTGTAGACCGGTACCTGCGAAGGAGGCTGGTCCCCAGATACAAGCTG
CAGCTCCTGGGTATTGCCTGCATGGTCATCTGTACCCGGTTCATCAGCAAAGAGATTCTGACAATTAGAG
AGGCTGTGTGGCTCACAGACAACACGTATAAATACGAGGACTTGGTGCGCGTGATGGGGGAGATCATCTC
CGCCCTGGAAGGGAAGATTCGGATCCCTACCGTGGTTGACTATAAAGAGGTCCTGCTGACACTGGTCCCC
GTCGCACCCAGAACCCAGCACCTGTGCAGCTTCCTCTGTGAGCTCACCCTGCTGCACACCAGCCTGTCCA
TCTATGCCCCAGCCCGCCTGGCCTCTGCGGCCCTTCTCCTGGCCAGACTCATGCATGGGCAGACACAGCC
CTGGACCACTCATCTGTGGGACCTCACTGGCTTCTCCTACAGTGACCTCGTACCCTGTGTCCTGAGCCTT
CATAAGAAGTGTTTCCACGATGATGCACCCAAAGACTACAGACAAATCTCTCTGACGGCCGTGAAGCAGC
GGTTCGAGGATAAGTGCTATGAGGAAATCAGCCGGGAAGAGGTGCTGAGCTATGCTGACTTGTGCAGTGC
AATAGGAGTAAAACAGGAAAGCCCAGAGCCCCCATCTTTCCCTAGCTCAGGGGAGATCCACACCTTCCTC
AGCTCGCCCTCCGGGAGGAGGAGCAAACGGAAGCGAGAGAACAGCCTTCAGGAGGACAGAGGCAGCTTTG
TCACCACACCCACCGCAGAGCTATCGAATCAGGAGGAGACATTGCTAGGCAGCCTTCTGGACTGGAGCCT
GGAGTGCTGCTCTGGCTATGAGGGAGACCAGGAGAGCGAAGGCGAGAAGGAGGGTGATGTCACAGCTCCC
AGCAGACTCCTTGATGTCACTGTGGTCTACCTGAACCCAGAAGAACATTGCTGCCAGGAGTCCAGTGATG
AGGAGGCCTGGCCAGAGGACAAGATCCACCCAGCACCAGGCACCCAGGCACCTCCAGCCTCAGCTCCCAG
GCCCCTTCTCTGCAATCGGGGAGATCGGGCCAAGGACATTACGACCTCAGGATACTCCTCTGTTAGCAGC
TCGAGTCCCATAAGCTCCCTGGATGGTGGCATGGGGGGCTCTCCCCAATCTACCTCAGTGCTCTCTGTGG
GCAGCCACTCAAGCACAAAGCCTTGCCACCATCAGGCCAAGAAGTCATGTTTACAGTGTCGTCCCCCAAA
TTCCCCAGAGAGCGGTGTTCACCAGCAGCCTGTAAAGCGCCAAAACCTGTCTGTACACAGTGACAAGGAC
ACGCACCTGGCTTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210593 protein sequence
Red=Cloning site Green=Tags(s)

MGSGGVIHCRCAKCFCYPTKRRIKRRPRNLTILSLPEDVLFHILKWLSVGDILAVRAVHSHLKYLVDNHA
SVWASASFQELWPSPQNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSVSDEACAEVNGLKASRFFSMAER
LNTGSEPFIWLFIRPPWSVSGSCCKAVVHDSLRAECQLQRSHKASILHCLGRVLNLFEDEEKRKQARSLL
EESSRQGCLISSYLLWESDRKMSDPGRCLHSFRKLRDYAAKGCWEAQLALAKACAGGSQLGLEGKACSES
VCQLFQASQAVNKQQIFSVQKGLSDTMRYILIDWLVEVATMKDFTSLCLHLTVECVDRYLRRRLVPRYKL
QLLGIACMVICTRFISKEILTIREAVWLTDNTYKYEDLVRVMGEIISALEGKIRIPTVVDYKEVLLTLVP
VAPRTQHLCSFLCELTLLHTSLSIYAPARLASAALLLARLMHGQTQPWTTHLWDLTGFSYSDLVPCVLSL
HKKCFHDDAPKDYRQISLTAVKQRFEDKCYEEISREEVLSYADLCSAIGVKQESPEPPSFPSSGEIHTFL
SSPSGRRSKRKRENSLQEDRGSFVTTPTAELSNQEETLLGSLLDWSLECCSGYEGDQESEGEKEGDVTAP
SRLLDVTVVYLNPEEHCCQESSDEEAWPEDKIHPAPGTQAPPASAPRPLLCNRGDRAKDITTSGYSSVSS
SSPISSLDGGMGGSPQSTSVLSVGSHSSTKPCHHQAKKSCLQCRPPNSPESGVHQQPVKRQNLSVHSDKD
THLAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007634
ORF Size 2325 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 3099 bp
RefSeq ORF 2334 bp
Locus ID 12449
UniProt ID P51944
Cytogenetics 17 12.32 cM
MW 86.4 kDa
Summary Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007634" in other vectors (4)
SKU Description Size Price
MC203265 Ccnf (untagged) - Mouse cyclin F (Ccnf), (10ug) 10 ug
$711.00
MG210593 Ccnf (tGFP-tagged) - Mouse cyclin F (Ccnf) 10 ug
$911.00
MR210593L3 Lenti ORF clone of Ccnf (Myc-DDK-tagged) - Mouse cyclin F (Ccnf) 10 ug
$1,011.00
MR210593L4 Lenti ORF clone of Ccnf (mGFP-tagged) - Mouse cyclin F (Ccnf) 10 ug
$1,011.00

Plasmid Map for MR210593

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210593
Circular map for MR210593

file-alt Citations

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