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Maged1 (NM_019791) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210587
Maged1 (Myc-DDK-tagged) - Mouse melanoma antigen, family D, 1 (Maged1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $710.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Maged1
Synonyms 2810433C11Rik; 5430405L04Rik; Dlixin; Dlxin-1; Dlxin1; DXBwg1492e; MAGE-D1; mFLJ00163; NRAGE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210587 representing NM_019791
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGAAACCGGACGGCGGTGCAGGCCTCCGCGGCTTCCAGGCTGAGGCCTCTGTTGAAGACAGCG
CCTTGCTTGTGCAGACCTTGATGGAAGCCATCCAGATCTCCGAGGCTCCGCCCACCAGCCAGGCCACAGC
AGCTGCCAGTGGGCCAAATGCTAGTCCCCAGAGTTCACAGCCCCCAACTGCCAATGAGAAGGCTGATACT
GAGGTTTCAGCAGCTGCTGCCAGGCCTAAGACAGGCTTTAAGGCCCAGAATGCCACCACAAAGGGGCCAA
ATGATTACTCTCAGGCACGTAATGCCAAGGAGATGCCCAAGAATCAGTCTAAGGCGGCCTTTAAGTCACA
GAATGGCACCCCTAAAGGTCCACATGCTGCCTCTGACTTTTCCCAGGCAGCACCCACAGGCAAATCAGCT
AAAAAGTCTGAAATGGCCTTTAAGGGTCAGAATAGCACTAAGGCTGGCCCCGGTACCACCTACAATTTCC
CTCAGTCTCCCAGTGCCAATGAGATGACCAACAACCAGCCTAAGACAGCTAAGGCTTGGAATGACACTAC
TAAGGTCCCTGGAGCTGATGCCCAGACCCAGAATGTAAATCAGGCCAAAATGGCTGACGTAGGGACCAGC
GCAGGTATCTCTGAAGCTGATGGTGCAGCAGCCCAGACATCAGCAGATGGCTCCCAGACTCAGAACGTGG
AGTCCCGGACTATAATTCGGGGCAAGAGGACCCGCAAGGTTAATAACTTGAATGTGGAAGAGAACAACAG
TGGGGATCAAAGGCGTGCCTCACTGGCCTCAGGGAACTGGAGGTCTGCTCCGGTTCCAGTGACCACTCAG
CAGAACCCACCTGGAGCACCCCCTAATGTGGTGTGGCAGACACCACTGGCTTGGCAGAACCCATCAGGCT
GGCAAAACCAGACAGCCAGGCAGACCCCACCAGCAGCACGTCAGAGTCCCCCAGCTAGGCAGACACCATC
AGCTTGGCAGAACCCAGTTGCATGGCAGAATCCAGTGATCTGGCCTAACCCAGTGATCTGGCAGAATCCA
GTGATCTGGCCAAACCCCATTGTCTGGCCTGGCCCAATTGTCTGGCCAAACCCAATGGCCTGGCAGAGTA
CACCTGGATGGCAGAGCCCACCCAGCTGGCAGGCTCCACCTAGTTGGCAGAGCCCTCAAGATTGGCAGGG
CCCTCCAGATTGGCAGGTACCACCTGACTGGTCAATGCCTCCTGACTGGTCCTTTCCCTCCGACTGGCCT
TTTCCACCTGACTGGATCCCCGCCGACTGGCCAATTCCACCTGACTGGCAGAACTTACGACCCTCACCTA
ATCTGAGATCCTCCTCCAACTCTCGTGCCTCACAGAACCAGGGTCCTCCACAGCCCCGAGATGTGGCCCT
TCTTCAGGAAAGAGCAAATAAGTTGGTCAAGTACCTGATGCTTAAAGACTACACGAAGGTGCCCATCAAG
CGCTCAGAAATGCTGAGGGATATCATCCGAGAATACACTGATGTCTATCCAGAAATCATTGAGCGCGCAT
GCTTTGTCCTGGAGAAGAAATTTGGAATCCAGCTGAAGGAAATCGACAAAGAAGAGCATCTGTATATTCT
CATCAGTACCCCTGAGTCCCTGGCTGGCATACTGGGAACGACCAAAGACACACCGAAGCTTGGCCTCCTC
TTAGTGATTCTGGGCATTATCTTCATGAATGGCAACCGTGCCACCGAGGCCGTCCTCTGGGAAGCACTGC
GCAAGATGGGACTACGTCCTGGGGTGAGACATCCCCTCCTTGGTGACCTGAGAAAACTTCTTACTTACGA
GTTTGTAAAGCAGAAATACCTGGACTATAGACGAGTGCCCAACAGCAACCCTCCTGAGTATGAGTTCCTC
TGGGGCCTCCGCTCCTACCATGAGACTAGCAAGATGAAAGTGCTGAGATTCATTGCAGAGGTTCAGAAGA
GAGACCCTCGTGACTGGACTGCACAGTTCATGGAAGCTGCAGATGAAGCCTTGGATGCTCTGGATGCTGC
TGCAGCTGAGGCAGAGGCCCGGGCTGAAGCGAGAAACCGCATGGGAATTGGAGATGAGGCTGTGTCTGGT
CCCTGGAGCTGGGATGACATTGAGTTTGAGCTGCTGACCTGGGATGAGGAAGGAGATTTTGGAGATCCTT
GGTCCAGGATCCCCTTTACCTTCTGGGCCAGATACCACCAGAATGCCCGCTCCAGGTTTCCCCAGGCCTT
TACTGGCCCCATCATTGGCCCCAGTGGTACTGCCACCGCCAACTTCGCCGCCAACTTCGGTGCCATTGGC
TTCTTCTGGGTTGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210587 representing NM_019791
Red=Cloning site Green=Tags(s)

MAQKPDGGAGLRGFQAEASVEDSALLVQTLMEAIQISEAPPTSQATAAASGPNASPQSSQPPTANEKADT
EVSAAAARPKTGFKAQNATTKGPNDYSQARNAKEMPKNQSKAAFKSQNGTPKGPHAASDFSQAAPTGKSA
KKSEMAFKGQNSTKAGPGTTYNFPQSPSANEMTNNQPKTAKAWNDTTKVPGADAQTQNVNQAKMADVGTS
AGISEADGAAAQTSADGSQTQNVESRTIIRGKRTRKVNNLNVEENNSGDQRRASLASGNWRSAPVPVTTQ
QNPPGAPPNVVWQTPLAWQNPSGWQNQTARQTPPAARQSPPARQTPSAWQNPVAWQNPVIWPNPVIWQNP
VIWPNPIVWPGPIVWPNPMAWQSTPGWQSPPSWQAPPSWQSPQDWQGPPDWQVPPDWSMPPDWSFPSDWP
FPPDWIPADWPIPPDWQNLRPSPNLRSSSNSRASQNQGPPQPRDVALLQERANKLVKYLMLKDYTKVPIK
RSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLL
LVILGIIFMNGNRATEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFL
WGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARNRMGIGDEAVSG
PWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQAFTGPIIGPSGTATANFAANFGAIG
FFWVE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019791
ORF Size 2325 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019791.3
RefSeq Size 2752 bp
RefSeq ORF 2328 bp
Locus ID 94275
UniProt ID Q9QYH6
Cytogenetics X 41.56 cM
MW 85.7 kDa
Summary Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rhythm regulation. May act as RORA coregulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.UniProtKB/Swiss-Prot Function
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"NM_019791" in other vectors (4)
SKU Description Size Price
MC202883 Maged1 (untagged) - Mouse melanoma antigen, family D, 1 (Maged1), (10ug) 10 ug
$711.00
MG210587 Maged1 (tGFP-tagged) - Mouse melanoma antigen, family D, 1 (Maged1) 10 ug
$910.00
MR210587L3 Lenti ORF clone of Maged1 (Myc-DDK-tagged) - Mouse melanoma antigen, family D, 1 (Maged1) 10 ug
$1,010.00
MR210587L4 Lenti ORF clone of Maged1 (mGFP-tagged) - Mouse melanoma antigen, family D, 1 (Maged1) 10 ug
$1,010.00

Plasmid Map for MR210587

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210587
Circular map for MR210587

file-alt Citations

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