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Ampd3 (NM_009667) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210541
Ampd3 (Myc-DDK-tagged) - Mouse adenosine monophosphate deaminase 3 (Ampd3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $701.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ampd3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210541 representing NM_009667
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCGCCAGTTCCCCAAGTTGAACATGTCCGATTTGGACGAACATGTCCGGCTGTTGGCGGAGAAGG
TGTTTGCTAAGGTGCTCCGAGAAGAGGACAGCAAAGATGTCATGTCCCTGTTTACTGTCCCCGAGGACTG
TCCCATCGGACAGAAGGAAGCCAAGGAGAGGGAGCTACAGAAGGAGCTTGCAGAGCAGAAGTCTGTGGAG
ACAGCAAAAAGAAAGAAGAGTTTCAAGATGATCCGGTCGCAGTCTTTGTCCCTGCAGATGCCAACACAAC
AAGACTGGAAAGGTCCTCCAACTGCCAGTCCTGCCATGTCTCCTGCAACCCCTCTGGTTCCCGGAGCTAC
TTCTAAGCCTGGGCCAGCACCCTATGCTATGCCTGAGTATCAGCGGGTCACCATCAGTGGAGATTACTGT
GCAGGGATCACTGTGGAAGACTATGAGCAGGCTGCCAAGAGCCTGGCCAAGGCCCTGATGATCCGGGAGA
AGTATGCACGGCTTGCCTACCACCGCTTCCCACGGACCACAGCCCAGTACCTGGCTCATCAGGGGGAAAG
TGTGCCTTTGGAAGAGGGTCTCCCAGACTTCCACCCTCCCCCACTGCCCCAGGAAGACCCTTATTGCCTG
GATGATGCACCCCCCAACCTGGGCTACCTGGTTCGCATGCACGGAGGTGTTCTCTTTGTGTATGACAATC
AGACGATGTTGGAACGCCAGGAGCCCCACAGCCTGCCCTACCCTGACCTGGAGACCTACATTGTGGACAT
GAGCCATATTCTGGCACTCATCACTGATGGCCCCACGAAAACCTATTGTCACCGGCGACTGAACTTTCTG
GAATCCAAGTTTAGCCTTCATGAGATGTTAAATGAAATGTCAGAGTTCAAGGAATTGAAGAGTAACCCCC
ACAGAGACTTTTATAATGTGAGAAAGGTAGACACACACATTCATGCAGCGGCCTGCATGAATCAGAAGCA
CCTGCTGCGTTTCATCAAGCACACGTACCAGACAGAGCCCGACAGAACTGTGGCGGAGAAGCTAGGTCGG
AAGATCACCCTGCGGCAGGTGTTTGACAGCCTGCACATGGACCCCTACGACCTCACGGTGGACTCGCTGG
ATGTTCATGCAGGCCGCCAGACATTTCACCGCTTCGACAAGTTCAACTCCAAGTACAACCCCGTGGGGGC
CAGCGAGCTCCGGGACCTGTATCTGAAAACTGAGAACTACCTCGGGGGAGAATACTTTGCTCGCATGGTC
AAGGAAGTGGCCCGGGAGCTAGAGGACAGCAAGTACCAATACTCAGAACCCCGGCTCTCCATCTATGGTC
GCAGTCCCAAGGAGTGGTCTAGCCTGGCCCGCTGGTTCATCCAGCACAAGGTCTACTCACCCAACATGCG
CTGGATCATCCAGGTGCCCCGCATCTATGATATATTTAGGTCAAAGAAACTACTGCCAAACTTTGGGAAG
ATGCTGGAGAACATCTTCCTGCCCCTGTTCAAAGCTACCATCAACCCCCAAGATCATCGGGAGCTTCACC
TCTTCCTTAAATACGTGACTGGGTTTGACAGTGTGGATGACGAATCCAAGCACAGTGACCACATGTTCTC
AGACAAGAGTCCCAGCCCAGACCTCTGGACCAGCGAGCAGAACCCGCCCTATAGTTACTACCTGTACTAC
ATGTATGCCAACATCATGGTGCTGAACAACCTCCGCAGGGAACGAGGCCTGAGCACGTTCCTCTTCCGGC
CTCACTGTGGGGAGGCCGGCTCCATCACTCACCTAGTGTCTGCCTTCCTGACGGCAGACAACATTTCCCA
CGGGCTGCTGCTTAAGAAGAGCCCTGTTTTGCAGTACCTCTACTACCTTGCTCAGATCCCTATTGCCATG
TCTCCTCTCAGCAACAACAGCCTGTTTCTGGAATATTCCAAGAACCCCCTCCGGGAATTCCTGCACAAGG
GACTACATGTCTCTCTCTCTACCGATGACCCCATGCAGTTTCACTACACAAAGGAAGCGCTCATGGAGGA
GTACGCCATTGCGGCACAAGTGTGGAAGCTGAGTACCTGCGACCTGTGTGAGATCGCCAGGAACAGTGTG
CTGCAGAGTGGCCTCTCACATCAGGAGAAGCAGAAGTTTCTGGGTCAGAATTATTACAAGGAAGGACCAG
AGGGCAATGACATTCGAAAGACAAATGTTGCACAGATCCGGATGGCATTCCGCTACGAGACCCTGTGTAA
TGAGCTCAGCTTTCTGTCCGATGCCATGAAGTCCGAGGAGATCACAGCCCTGACCAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210541 representing NM_009667
Red=Cloning site Green=Tags(s)

MPRQFPKLNMSDLDEHVRLLAEKVFAKVLREEDSKDVMSLFTVPEDCPIGQKEAKERELQKELAEQKSVE
TAKRKKSFKMIRSQSLSLQMPTQQDWKGPPTASPAMSPATPLVPGATSKPGPAPYAMPEYQRVTISGDYC
AGITVEDYEQAAKSLAKALMIREKYARLAYHRFPRTTAQYLAHQGESVPLEEGLPDFHPPPLPQEDPYCL
DDAPPNLGYLVRMHGGVLFVYDNQTMLERQEPHSLPYPDLETYIVDMSHILALITDGPTKTYCHRRLNFL
ESKFSLHEMLNEMSEFKELKSNPHRDFYNVRKVDTHIHAAACMNQKHLLRFIKHTYQTEPDRTVAEKLGR
KITLRQVFDSLHMDPYDLTVDSLDVHAGRQTFHRFDKFNSKYNPVGASELRDLYLKTENYLGGEYFARMV
KEVARELEDSKYQYSEPRLSIYGRSPKEWSSLARWFIQHKVYSPNMRWIIQVPRIYDIFRSKKLLPNFGK
MLENIFLPLFKATINPQDHRELHLFLKYVTGFDSVDDESKHSDHMFSDKSPSPDLWTSEQNPPYSYYLYY
MYANIMVLNNLRRERGLSTFLFRPHCGEAGSITHLVSAFLTADNISHGLLLKKSPVLQYLYYLAQIPIAM
SPLSNNSLFLEYSKNPLREFLHKGLHVSLSTDDPMQFHYTKEALMEEYAIAAQVWKLSTCDLCEIARNSV
LQSGLSHQEKQKFLGQNYYKEGPEGNDIRKTNVAQIRMAFRYETLCNELSFLSDAMKSEEITALTK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009667
ORF Size 2298 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009667.3, NP_033797.2
RefSeq Size 4098 bp
RefSeq ORF 2301 bp
Locus ID 11717
UniProt ID O08739
Cytogenetics 7 57.85 cM
MW 88.7 kDa
Summary This gene encodes a member of the adenosine and AMP deaminases family. The encoded protein is an AMP deaminase involved in nucleotide and energy metabolism in erythrocytes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2013
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Documents
Resources
"NM_009667" in other vectors (4)
SKU Description Size Price
MC205224 Ampd3 (untagged) - Mouse adenosine monophosphate deaminase 3 (Ampd3), (10ug) 10 ug
$702.00
MG210541 Ampd3 (tGFP-tagged) - Mouse AMP deaminase 3 (Ampd3) 10 ug
$901.00
MR210541L3 Lenti ORF clone of Ampd3 (Myc-DDK-tagged) - Mouse adenosine monophosphate deaminase 3 (Ampd3) 10 ug
$1,001.00
MR210541L4 Lenti ORF clone of Ampd3 (mGFP-tagged) - Mouse adenosine monophosphate deaminase 3 (Ampd3) 10 ug
$1,001.00

Plasmid Map for MR210541

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210541
Circular map for MR210541

file-alt Citations

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