Sec23a (NM_009147) Mouse Tagged ORF Clone
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Sec23a |
| Synonyms | Msec23; Sec23r |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009147 |
| ORF Size | 2295 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009147.2, NP_033173.2 |
| RefSeq Size | 4108 bp |
| RefSeq ORF | 2298 bp |
| Locus ID | 20334 |
| UniProt ID | Q01405 |
| Cytogenetics | 12 C1 |
| MW | 86.2 kDa |
| Summary | Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (By similarity). Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane (PubMed:27354378).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009147" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC204137 | Sec23a (untagged) - Mouse SEC23A (S. cerevisiae) (Sec23a), (10ug) | 10 ug |
$701.00
|
|
| MG210540 | Sec23a (tGFP-tagged) - Mouse SEC23A (S. cerevisiae) (Sec23a) | 10 ug |
$901.00
|
|
| MR210540L3 | Lenti ORF clone of Sec23a (Myc-DDK-tagged) - Mouse SEC23A (S. cerevisiae) (Sec23a) | 10 ug |
$1,001.00
|
|
| MR210540L4 | Lenti ORF clone of Sec23a (mGFP-tagged) - Mouse SEC23A (S. cerevisiae) (Sec23a) | 10 ug |
$1,001.00
|
|
Citations
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