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Plcd1 (NM_019676) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210488
Plcd1 (Myc-DDK-tagged) - Mouse phospholipase C, delta 1 (Plcd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $693.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Plcd1
Synonyms AW212592; C79986
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210488 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCCGGTCGGGACTTCCTGACCTTGCACGGGCTGCAGGATGACCCGGACCTTCAGGCCCTCCTGA
AGGGCAGCCAGCTTCTGAAGGTGAAGTCCAGCTCGTGGCGTAGAGAACGCTTCTACAAGCTACAGGAGGA
CTGCAAGACCATCTGGCAGGAATCTCGAAAGGTCATGAGGTCCCCGGAGTCGCAGCTGTTCTCCATCGAG
GACATTCAGGAGGTGCGGATGGGACACCGCACAGAAGGCCTGGAGAAATTTGCCAGAGACATACCCGAGG
ACCGATGCTTCTCCATCGTCTTCAAGGACCAGCGCAATACCCTAGACCTCATCGCCCCGTCTCCAGCTGA
CGTCCAGCATTGGGTGCAGGGCCTGCGCAAGATCATCGACCGCTCGGGCTCCATGGACCAGCGGCAGAAG
CTGCAGCACTGGATTCACTCCTGCTTGCGAAAGGCTGATAAAAACAAGGATAACAAGATGAACTTCAAGG
AGGTGAAGGACTTCCTGAAGGAGCTCAACGTACAGGTGGATGACAGCTACGCCAGGAAGATCTTCAGGGA
ATGTGACCACTCCCAGACAGACTCGCTGGAGGATGAGGAGATTGAGACCTTCTACAGGATGCTGACCCAG
CGGGTAGAGATCGACCGTGCCTTTGCAGAGGCCGCAGGTTCTGCAGAGACCCTGTCGGTGGAGAAGCTAG
TGACGTTCCTGCAGCATCAGCAGCGGGAGGAGGAGGCGGGGCCAGCCTTGGCCCTCTCTCTCATTGAGCG
CTACGAGCCCAGTGAGACTGCCAAAGCCCAGCGGCAGATGACCAAGGATGGCTTCCTCATGTACCTGCTG
TCTGCTGACGGTAACGCCTTCAGCCTGGCGCACCGCCGTGTCTACCAGGACATGAACCAGCCGCTCAGCC
ACTACTTAGTGTCTTCTTCCCACAACACCTACCTACTGGAGGACCAGCTCACAGGGCCCAGCAGCACGGA
GGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGTCTGGAGCTGGACTGCTGGGACGGCCCCAACCAG
GAACCCATCATCTACCACGGCTACACTTTTACCTCTAAGATACTTTTCTGCGACGTGCTCAGGGCCATCC
GGGACTACGCCTTCAAGGCATCTCCCTACCCAGTTATCCTGTCCCTGGAGAACCACTGCAGCCTGGAGCA
ACAACGAGTCATGGCTCATCACCTGAGGGCCATCCTGGGGCCCATGCTGTTGGACCAGCCGCTGGATGGG
GTTACCACGAGCCTGCCTTCGCCTGAGCAACTGAAGGAAAAGATTCTGTTGAAAGGGAAGAAGCTGGGCG
GGCTGCTACCTGCTGGAGGGGAAAACGGGCCTGAAGCCACCGATGTGTCTGACGAAGATGAGGCTGCTGA
AATGGAAGATGAGGCCGTGCGCAGCCAAGTGCAACACAAGCCCAAGGAGGATAAGCTAAAGCTGGTGCCG
GAGCTCTCCGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGTGGCTTCTCCAGTCCTAGCACCTCTG
GGCAGGCATTCTATGAGATGGCTTCCTTCTCCGAGAGCCGTGCCCTGCGGCTGCTGCAAGAATCAGGAAA
TAGTTTTGTCCGCCATAACGTGGGCCATCTGAGCAGGATCTACCCAGCTGGGTGGAGGACAGATTCCTCC
AACTACAGTCCCGTGGAGATGTGGAATGGAGGCTGCCAGATTGTGGCTCTGAACTTCCAAACCCCTGGGC
CAGAAATGGATGTTTACCTTGGCTGCTTCCAGGACAATGGAGGTTGTGGGTATGTGCTGAAACCGGCCTT
CCTGAGAGACCCTGACACCACCTTCAACTCACGGGCCCTGACTCAGGGGCCCTGGTGGGCCCCAAAGAAG
CTCAGAGTCTGGATCATCTCTGGACAGCAGCTGCCGAAGGTCAATAAGAATAAGAATTCTATCGTGGACC
CCAAGGTGATAGTGGAGATCCACGGTGTGGGCCAGGATGTGGCCAGCCGTCAGACAGCAGTTATTACCAA
TAATGGTTTCAACCCACGGTGGGACACGGAGTTTGAGTTTGTGGTAGCAGTGCCTGACCTTGCCCTTGTA
CGTTTCATGGTAGAGGATTATGACTCCTCCTCTAAGAACGACTTTATCGGCCAGAGTACTATCCCCTGGA
ACAGCCTCAAGCAGGGGTACCGCCATGTCCACCTCTTGTCTAAGAATGGAGACCTGCATCCATCAGCCAC
TCTCTTTGTGAAGATCTCCATCCAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210488 protein sequence
Red=Cloning site Green=Tags(s)

MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIE
DIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQK
LQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQ
RVEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLL
SADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQ
EPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDG
VTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVP
ELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQESGNSFVRHNVGHLSRIYPAGWRTDSS
NYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPDTTFNSRALTQGPWWAPKK
LRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAVITNNGFNPRWDTEFEFVVAVPDLALV
RFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHPSATLFVKISIQD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019676
ORF Size 2268 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019676.1, NM_019676.2, NM_019676.3, NP_062650.1
RefSeq Size 2657 bp
RefSeq ORF 2271 bp
Locus ID 18799
UniProt ID Q8R3B1
Cytogenetics 9 F3
MW 85.9 kDa
Summary The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.UniProtKB/Swiss-Prot Function
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Documents
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"NM_019676" in other vectors (4)
SKU Description Size Price
MC203035 Plcd1 (untagged) - Mouse phospholipase C, delta 1 (Plcd1), (10ug) 10 ug
$693.00
MG210488 Plcd1 (tGFP-tagged) - Mouse phospholipase C, delta 1 (Plcd1) 10 ug
$789.00 $893.00
MR210488L3 Lenti ORF clone of Plcd1 (Myc-DDK-tagged) - Mouse phospholipase C, delta 1 (Plcd1) 10 ug
$993.00
MR210488L4 Lenti ORF clone of Plcd1 (mGFP-tagged) - Mouse phospholipase C, delta 1 (Plcd1) 10 ug
$993.00

Plasmid Map for MR210488

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210488
Circular map for MR210488

file-alt Citations

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