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Sema3c (NM_013657) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210467
Sema3c (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (Sema3c)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $689.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sema3c
Synonyms 1110036B02Rik; Semae; SemE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210467 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTCCGGGCGATTTGTGTGTTGGTTGGAGTATTTATTTGTTCCATTTGTGTACGAGGATCTTCCC
AGCCCCAAGCAAGAGTTTATTTAACATTTGATGAGCTTCGAGAAACCAAAACCTCTGAGTACTTTAGTCT
GTCCCACCAGCAGTTAGACTACAGAATATTGCTGATGGATGAAGATCAAGACCGGATATATGTGGGGAGC
AAAGACCACATCCTGTCTTTGAATATCAACAATATCAGTCAAGAACCTTTGAGTGTTTTCTGGCCAGCAT
CAACAATCAAAGTTGAAGAGTGCAAAATGGCTGGCAAAGATCCTACACATGGCTGTGGAAATTTCGTCCG
GGTGATTCAGACATTCAACCGTACTCACCTGTATGTCTGTGGGAGTGGAGCGTTCAGCCCAGTGTGCACC
TACCTGAACCGGGGAAGGAGGTCAGAGGACCAGGTATTCATGATCGACTCTAAGTGTGAATCTGGCAAAG
GACGATGCTCTTTCAACCCGAATGTGAACACTGTGTCTGTTATGATCAATGAGGAACTCTTCTCAGGAAT
GTATATAGACTTCATGGGAACAGATGCTGCTATTTTCCGAAGTTTAACTAAGAGGAATGCAGTTCGAACT
GATCAACATAATTCAAAATGGCTGAGTGAACCCATGTTTGTGGACGCACATGTGATCCCAGATGGCACTG
ATCCAAATGATGCTAAGGTCTATTTCTTCTTCAAAGAAAGACTGACTGACAACAATAGGAGCACAAAACA
GATTCATTCCATGATTGCAAGAATATGCCCTAATGACACTGGTGGACAACGTAGTCTTGTCAACAAGTGG
ACCACATTCTTAAAGGCAAGACTTGTATGCTCAGTCACAGATGAAGATGGCCCAGAGACACATTTTGATG
AACTAGAGGATGTCTTTCTGCTGGAAACTGACAATCCAAGGACAACACTCGTGTATGGCATCTTCACCAC
ATCAAGCTCTGTTTTTAAGGGATCGGCAGTGTGTGTGTATCATTTATCTGATATACAGACTGTATTCAAT
GGGCCCTTTGCCCACAAGGAAGGGCCCAATCACCAGCTGATCTCCTATCAAGGTAGAATCCCATATCCTC
GCCCAGGAACTTGCCCAGGAGGGGCCTTTACACCCAATATGAGAACCACCAAGGACTTCCCAGATGACGT
TGTCACTTTTATTCGGAACCACCCTCTCATGTACAATTCCATCTACCCCATCCACAGAAGGCCTCTGATA
GTCCGCATAGGCACTGACTACAAGTACACAAAGATTGCTGTGGACCGTGTCAACGCTGCTGATGGGAGAT
ACCACGTTCTGTTTCTGGGCACAGATCGGGGCACCGTGCAGAAGGTCGTAGTCCTTCCTACCAATAGCTC
TGCCAGTGGGGAACTCATCCTGGAGGAGCTGGAAGTCTTCAAGAATCATGTTCCCATAACAACAATGAAA
ATCTCATCCAAAAAGCAACAGTTGTACGTGAGCTCCAATGAGGGGGTTTCCCAAGTCTCTCTGCATCGCT
GCCATATCTACGGCACAGCCTGTGCGGACTGCTGCTTGGCGAGGGATCCATACTGTGCCTGGGATGGCCA
CTCTTGCTCTAGGTTCTACCCCACTGGGAAGCGGAGGAGCCGAAGACAAGATGTGAGACATGGAAACCCA
CTGACACAATGCCGAGGGTTCAATCTGAAAGCATACAGAAATGCAGCTGAAATTGTTCAGTATGGAGTAA
GAAATAACAGCACTTTCCTTGAGTGTGCTCCCAAGTCTCCACAGGCATCTATCAAGTGGTTGCTGCAGAA
AGACAAAGACAGGAGGAAGGAGGTTAAACTGAACGAGCGCATTATAGCTACTTCCCAAGGACTACTGATT
CGCTCTGTTCAAGATTCTGACCAAGGACTCTACCACTGCATTGCCACTGAGAACAGCTTCAAACAGACCA
TAGCCAAGATCAACTTCAAAGTTTTAGATTCAGAAATGGTGGCCGTTGTGACAGACAAGTGGTCCCCGTG
GACATGGGCTGGCTCTGTGAGGGCTCTACCCTTCCATCCAAAGGACATCCTGGGGGCATTCAGCCACTCG
GAAATGCAGCTCATCAATCAGTACTGCAAAGACACCCGGCAGCAGCAGCAGCTGGGGGAAGAACCACAGA
AGATGAGAGGGGACTATGGCAAGCTGAAGGCTCTCATCAACAGCAGGAAAAGCAGAAACAGGAGGAATCA
GCTTCCAGAGTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210467 protein sequence
Red=Cloning site Green=Tags(s)

MAFRAICVLVGVFICSICVRGSSQPQARVYLTFDELRETKTSEYFSLSHQQLDYRILLMDEDQDRIYVGS
KDHILSLNINNISQEPLSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQTFNRTHLYVCGSGAFSPVCT
YLNRGRRSEDQVFMIDSKCESGKGRCSFNPNVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRT
DQHNSKWLSEPMFVDAHVIPDGTDPNDAKVYFFFKERLTDNNRSTKQIHSMIARICPNDTGGQRSLVNKW
TTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTLVYGIFTTSSSVFKGSAVCVYHLSDIQTVFN
GPFAHKEGPNHQLISYQGRIPYPRPGTCPGGAFTPNMRTTKDFPDDVVTFIRNHPLMYNSIYPIHRRPLI
VRIGTDYKYTKIAVDRVNAADGRYHVLFLGTDRGTVQKVVVLPTNSSASGELILEELEVFKNHVPITTMK
ISSKKQQLYVSSNEGVSQVSLHRCHIYGTACADCCLARDPYCAWDGHSCSRFYPTGKRRSRRQDVRHGNP
LTQCRGFNLKAYRNAAEIVQYGVRNNSTFLECAPKSPQASIKWLLQKDKDRRKEVKLNERIIATSQGLLI
RSVQDSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVVTDKWSPWTWAGSVRALPFHPKDILGAFSHS
EMQLINQYCKDTRQQQQLGEEPQKMRGDYGKLKALINSRKSRNRRNQLPES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013657
ORF Size 2253 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013657.5, NP_038685.3
RefSeq Size 4956 bp
RefSeq ORF 2256 bp
Locus ID 20348
UniProt ID Q62181
Cytogenetics 5 A3
MW 85.3 kDa
Summary Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013657" in other vectors (4)
SKU Description Size Price
MC204776 Sema3c (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (Sema3c), (10ug) 10 ug
$689.00
MG210467 Sema3c (tGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (Sema3c) 10 ug
$889.00
MR210467L3 Lenti ORF clone of Sema3c (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (Sema3c) 10 ug
$989.00
MR210467L4 Lenti ORF clone of Sema3c (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (Sema3c) 10 ug
$989.00

Plasmid Map for MR210467

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210467
Circular map for MR210467

file-alt Citations

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