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Phtf2 (NM_172992) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210442
Phtf2 (Myc-DDK-tagged) - Mouse putative homeodomain transcription factor 2 (Phtf2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $684.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Phtf2
Synonyms 1110054G21Rik; 9530062N20Rik; AI447096; C77923
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210442 representing NM_172992
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCAGAGTCACAGATGCTATAGTCTGGTATCAGAAGAAGATTGGAGCCTATGATCAACAAATAT
GGGAAAAGTCTGTTGAACAAAGAGAAATCAAGGGCTTAAGGAATAAACCAAAGAAAACAGCACATGTGAA
ACCAGACCTCATCGATGTTGATCTTGTGAGAGGGTCTGCATTTGCTAAGGCAAAGCCTGAAAGTCCTTGG
ACTTCTCTGACAAGGAAAGGGATTGTTCGAGTTGTCTTTTTCCCTTTCTTCTCCCGCTGGTGGTTACAAG
TAACGTCCAGAGTCATCTTCTCCTGGCTTCTTGTTCTCTATCTTCTCCAAGTTGCTGCAATAGTATTATT
CTGTTCCGCTCCTAGCCCACATAGCATACCTCTAACTGAAGTGATTGGGCCAATATGGCTGATGCTGCTT
TTGGGAACCGTGCATTGCCAGATTGTTTCAACAAGAACACCCAAGCCTCCACTAGGCACAGGCGGTAAAC
GAAGAAGGAAATTAAGAAAAGCAGCCCATTTGGAAGTCCACAGGGAAGGGGACGGTTCTAGCACCACAGA
TAACACACAGGAGGGAGCGGTGCAGAGCCACGGCGCAGGCGCCCCTTACAGTGTTGGCACTGTCTTCAGA
GACCTTTGGCTTGCTGCTTTCTTCTTATCAGGGTCAAAGAAGGCAAAGAATTCAATTGATAAGTCAACCG
AGACTGACAATGGCTATGTGTCCCTCGATGGGAAGAGGACTGTAAAAAGCAGTGAGGATGGAGCACAGTA
CCATGAGCCTCAGTGTGAAACTGTTGGACCAGAAGACGCAGCCTGGGCCACACGAACGCCAAGGAGTGTT
CCTGCCAAAGATACCCAAAGAAAGATAACAAATGTCTCTGATGAAGTATCCAGTGAGGAGGGTCCCGAAA
CAGGATACCCATTGCGTGGTCATGTGGACAGAACTTCTGAAAGCGGTCTTCGAAATAGAAAGCCACACCA
TTATAAAAAGCATTATGCAAATGAGGATGCCCCTAAATCGGGTACTAGTTGCAGCTCTCGCTGTTCAAGT
TCCAGACAGGATTCTGAGAGCACAAGGCCAGAATCTGAAACCGAAGATGTATTATGGGAAGACTTGTTAC
ACTGTGCGGAATGCCGCTCATCTTGTACCAGTGAGACAGATGTGGGAAACCCTCAGATAAATCCATGTGG
GAAGAAAGAATATAGAGACGACCCTTTTCATCAGAGTCATTTGCCCTGGCTTCATAGTTCCCACCCAGGA
TTAGAAAAAATCAGTGCTATAGTGTGGGAAGGTAACGACTGTAAGAAAGCAGACATGTCTGTACTTGAAA
TCAGTGGGATGATAATGAACAGAGTGAACAACCATGTCCCAGGCATAGGATACCAGGTATTTGGAAATGC
CATCTCTCTAATCCTGGGCTTGACCCCATTTGTTTTCCGACTGTCACAAGCAACAGACTTGGAGCAGCTC
ACAGCCCATTCTGCGTCAGAACTTTATGTGATTGCGTTTGGCTCTAACGAAGATGTCATGGTTCTTTCTA
TGGTCCTGATAAGTTTTGTGGTTCGAGTCTCTCTTGTGTGGATCTTCTTTTTTTTGCTGTGTGTAGCAGA
AAGAACATATAAGCAACGATTACTCTTTGCAAAACTCTTTGGACACTTGACGTCTGCAAGGAGAGCTCGC
AAATCTGAGGTTCCGCATTTCCGGTTGAAGAAAGTACAGAATATAAAAATGTGGCTGTCTCTGCGTTCCT
ATCTTAAGCGCCGAGGCCCTCAGCGATCTGTGGATGTCATTGTCTCATCGGCCTTCTTACTGACAATCTC
AGTTGTGTTTATCTGCTGTGCACAGCTGCTCCACGTGCACGAGATCTTCCTTGACTGTCACTATAACTGG
GAGCTGGTGATCTGGTGCATCTCCTTAACGCTGTTCCTCCTGAGGTTCGTTACTCTTGGATCAGAAACCA
GCAAGAAATACAGCAATACCTCAATCCTACTTACGGAACAGATAAACCTCTACTTGAAAATGGAGAAGAA
ACCTAACAAAAAGGAGGAGCTGACCCTTGTGAATAATGTGTTAAAGCTGGCTACGAAACTGCTGAAGGAA
TTGGACAGTCCCTTTAGGTTGTATGGACTTACAATGAATCCGCTGCTGTATAACATCACCCAGGTTGTCA
TCCTTTCGGCTGTTTCCGGTGTCATCAGTGACTTGCTTGGATTTAATTTAAAGCTGTGGAAGATCAAATC
G

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210442 representing NM_172992
Red=Cloning site Green=Tags(s)

MASRVTDAIVWYQKKIGAYDQQIWEKSVEQREIKGLRNKPKKTAHVKPDLIDVDLVRGSAFAKAKPESPW
TSLTRKGIVRVVFFPFFSRWWLQVTSRVIFSWLLVLYLLQVAAIVLFCSAPSPHSIPLTEVIGPIWLMLL
LGTVHCQIVSTRTPKPPLGTGGKRRRKLRKAAHLEVHREGDGSSTTDNTQEGAVQSHGAGAPYSVGTVFR
DLWLAAFFLSGSKKAKNSIDKSTETDNGYVSLDGKRTVKSSEDGAQYHEPQCETVGPEDAAWATRTPRSV
PAKDTQRKITNVSDEVSSEEGPETGYPLRGHVDRTSESGLRNRKPHHYKKHYANEDAPKSGTSCSSRCSS
SRQDSESTRPESETEDVLWEDLLHCAECRSSCTSETDVGNPQINPCGKKEYRDDPFHQSHLPWLHSSHPG
LEKISAIVWEGNDCKKADMSVLEISGMIMNRVNNHVPGIGYQVFGNAISLILGLTPFVFRLSQATDLEQL
TAHSASELYVIAFGSNEDVMVLSMVLISFVVRVSLVWIFFFLLCVAERTYKQRLLFAKLFGHLTSARRAR
KSEVPHFRLKKVQNIKMWLSLRSYLKRRGPQRSVDVIVSSAFLLTISVVFICCAQLLHVHEIFLDCHYNW
ELVIWCISLTLFLLRFVTLGSETSKKYSNTSILLTEQINLYLKMEKKPNKKEELTLVNNVLKLATKLLKE
LDSPFRLYGLTMNPLLYNITQVVILSAVSGVISDLLGFNLKLWKIKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172992
ORF Size 2241 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172992.3, NP_766580.3
RefSeq Size 4839 bp
RefSeq ORF 2244 bp
Locus ID 68770
UniProt ID Q8CB19
Cytogenetics 5 A3
MW 84.6 kDa
Summary May play a role in transcription regulation.UniProtKB/Swiss-Prot Function
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"NM_172992" in other vectors (4)
SKU Description Size Price
MC221304 Phtf2 (untagged) - Mouse putative homeodomain transcription factor 2 (Phtf2), (10ug) 10 ug
$753.00
MG210442 Phtf2 (tGFP-tagged) - Mouse putative homeodomain transcription factor 2 (Phtf2) 10 ug
$884.00
MR210442L3 Lenti ORF clone of Phtf2 (Myc-DDK-tagged) - Mouse putative homeodomain transcription factor 2 (Phtf2) 10 ug
$984.00
MR210442L4 Lenti ORF clone of Phtf2 (mGFP-tagged) - Mouse putative homeodomain transcription factor 2 (Phtf2) 10 ug
$984.00

Plasmid Map for MR210442

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210442
Circular map for MR210442

file-alt Citations

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