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Adarb2 (NM_052977) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210425
Adarb2 (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific, B2 (Adarb2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $683.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adarb2
Synonyms Adar3; RED2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210425 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCTGTCCTGGGGAGTGGCAGAGGGTCTGGAGGGCTAAGCAGTCAACTCAAATGCAAGTCCAAGA
GGAGGAGGAGGAGGAGGTCCAAGAGGAAAGACAAAGTCAGCATACTGTCAACCTTCCTTGCTCCTTTCAA
GTACCTAAGTCCTGGCACCACAAACACAGAGGATGAAGATAACCTAAGTACCAGCAGTGCTGAAGTGAAG
GAAAACCGAAATGTGAGCAACCTGGGGACCAGGCCCCTGCCCCCTGGAGACTGGGCCAGAGGCAGCACAC
CCAGCGTTAAAAGGAAAAGGCCGCTGGAGGAAGGAAATGGGGGACATTTCTGCAAACTCCAGCTGATCTG
GAAGAAGCTCTCCTGGTCTGTGACACCCAAGAATGCGCTGGTCCAGCTGCATGAGCTGAAGCCTGGTCTG
CAGTACCGAATGGTGTCACAGACGGGCCCGGTGCACGCCCCAGTTTTCGCAGTGGCTGTAGAAGTGAACG
GCCTCACATTTGAAGGCACGGGGCCCACTAAGAAGAAGGCCAAGATGCGGGCAGCAGAAATGGCCCTGAA
GTCCTTTGTGCAGTTCCCCAATGCCTTCCAGGCCCACCTGGCCATGGGCAGTAGTACCAGCCCGTGCACT
GACTTCACCTCTGACCAGGCTGACTTCCCAGACACGTTGTTCAAGGAGTTTGAACCATCCTCAAAAAACG
AAGACTTCCCAGGCTGTCATCCCGTTGATACTGAGTTTCTGTCCTCTGCCTATCGGCGAGGACGGCTACT
CTACCACACCCTGGACCTCATGGGCCAGGCTCTGCCTGACAGGTCCAGGCTGGCTCCGGGAGCCTTGGGT
GAGAGGAACCCTGTAGTGGTACTGAATGAGTTACGCTCAGGTCTTCGGTATGTGTGCCTCTCCGAGACAG
CCGAGAAGCCCCGAGTCAAGAGCTTTGTCATGGCTGTGTGCGTGGATGGGAGGACGTTCGAAGGCTCTGG
ACGCAGCAAGAAGCTGGCCAAGGGCCAAGCAGCACAAGCTGCCTTGCAGGCCCTCTTCGATATCCGGCTG
CCTGGTCACATACCCAGCAGGAGTAAAAGCAACCTCTTGCCACAGGATTTTGCAGACTCTGTGTCCCAGC
TGGTCACACAGAAGTTCCGTGAACTGACAGTCGGCCTGACATCTGTGTATGCCCGCCACAAAACACTGGC
AGGAATCGTCATGACCAAAGGCTTGGACACCAAACAAGCGCAGGTCATCGTTCTGTCCTCTGGGACCAAG
TGTATCAGCGGTGAGCACATCAGTGACCAGGGGCTGGTTGTCAACGACTGTCATGCTGAGATCGTGGCGA
GGCGGGCATTTCTTCACTTCCTCTACTCTCAGCTGGAGCTGCACTTGAGCAAGCATCAAGAGGACCCCGA
GAGATCAATATTCATACGTTTAAAGGAAGGAGGTTACAGGCTGCGAGAAAACATCCTCTTCCATCTCTAC
GTGAGCACATCCCCCTGTGGAGATGCCAGAGTCAACTCTCCATATGAAATCACCACAGACTTGAACAGCA
GCAAACACATCGTCAGGAAGTTCCGAGGGCACCTGCGCACCAAGATTGAGTCTGGGGAAGGAACAGTCCC
TGTGCGGGGCCCCAGTGCAGTCCAGACGTGGGATGGCATCCTATTGGGGGAGCAGCTAATCACCATGTCC
TGCACAGACAAGATTGCCAGCTGGAATGTGCTGGGACTACAAGGCGCTCTCCTCTGTCACTTCATCGAGC
CTGTGTACCTCCACAGCATCATTGTGGGCAGCCTGCACCACACGGGCCATCTTGCACGGGTCATGAGCCA
CAGGATGGAGGGAATCGGCCAGCTGCCTGCCTCATACCGACAAAACAGGCCTCTCCTTAGTGGCGTGAGT
CACGCAGAGGCCCGGCAGCCAGGAAAGTCTCCCCACTTCAGTGCCAACTGGGTCGTGGGCAGCGCCGACC
TGGAGATCATTAATGCCACCACTGGGAAGAGGAGCTGTGGGGGCTCATCCCGGCTCTGCAAACACGTGTT
TTCTGCTTGGTGGGCACGGCTGCATGGTAGGCTAAGCACACGGATCCCCAGCCATGGAGACACACCATCC
ATGTACTGTGAGGCCAAGCAGGGGGCTCACACCTACCAGTCTGTTAAACAGCAGCTCTTCAAGGCCTTTC
AGAAAGCCGGTCTTGGCACCTGGGTGAGAAAACCACCGGAGCAAGATCAGTTTCTACTAAGTCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210425 protein sequence
Red=Cloning site Green=Tags(s)

MASVLGSGRGSGGLSSQLKCKSKRRRRRRSKRKDKVSILSTFLAPFKYLSPGTTNTEDEDNLSTSSAEVK
ENRNVSNLGTRPLPPGDWARGSTPSVKRKRPLEEGNGGHFCKLQLIWKKLSWSVTPKNALVQLHELKPGL
QYRMVSQTGPVHAPVFAVAVEVNGLTFEGTGPTKKKAKMRAAEMALKSFVQFPNAFQAHLAMGSSTSPCT
DFTSDQADFPDTLFKEFEPSSKNEDFPGCHPVDTEFLSSAYRRGRLLYHTLDLMGQALPDRSRLAPGALG
ERNPVVVLNELRSGLRYVCLSETAEKPRVKSFVMAVCVDGRTFEGSGRSKKLAKGQAAQAALQALFDIRL
PGHIPSRSKSNLLPQDFADSVSQLVTQKFRELTVGLTSVYARHKTLAGIVMTKGLDTKQAQVIVLSSGTK
CISGEHISDQGLVVNDCHAEIVARRAFLHFLYSQLELHLSKHQEDPERSIFIRLKEGGYRLRENILFHLY
VSTSPCGDARVNSPYEITTDLNSSKHIVRKFRGHLRTKIESGEGTVPVRGPSAVQTWDGILLGEQLITMS
CTDKIASWNVLGLQGALLCHFIEPVYLHSIIVGSLHHTGHLARVMSHRMEGIGQLPASYRQNRPLLSGVS
HAEARQPGKSPHFSANWVVGSADLEIINATTGKRSCGGSSRLCKHVFSAWWARLHGRLSTRIPSHGDTPS
MYCEAKQGAHTYQSVKQQLFKAFQKAGLGTWVRKPPEQDQFLLSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052977
ORF Size 2235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052977.5, NP_443209.2
RefSeq Size 5633 bp
RefSeq ORF 2238 bp
Locus ID 94191
UniProt ID Q9JI20
Cytogenetics 13 A1
MW 82.2 kDa
Summary Lacks editing activity. It prevents the binding of other ADAR enzymes to targets in vitro, and decreases the efficiency of these enzymes. Capable of binding to dsRNA but also to ssRNA (By similarity).UniProtKB/Swiss-Prot Function
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"NM_052977" in other vectors (4)
SKU Description Size Price
MC221315 Adarb2 (untagged) - Mouse adenosine deaminase, RNA-specific, B2 (Adarb2), (10ug) 10 ug
$751.00
MG210425 Adarb2 (tGFP-tagged) - Mouse adenosine deaminase, RNA-specific, B2 (Adarb2) 10 ug
$883.00
MR210425L3 Lenti ORF clone of Adarb2 (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific, B2 (Adarb2) 10 ug
$983.00
MR210425L4 Lenti ORF clone of Adarb2 (mGFP-tagged) - Mouse adenosine deaminase, RNA-specific, B2 (Adarb2) 10 ug
$983.00

Plasmid Map for MR210425

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210425
Circular map for MR210425

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