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Nsf (NM_008740) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210421
Nsf (Myc-DDK-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $682.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nsf
Synonyms AI316878; AU020090; AU067812; SKD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210421 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCCGGACTATGCAAGCTGCGAGATGCCCTACTGATGAATTATCTTTAAGCAACTGTGCGGTTG
TGAACGAAAAAGATTTCCAGTCTGGCCAGCATGTGATGGTGAGGACGTCCCCCAATCACAAGTACATATT
TACGCTGAGGACCCATCCGTCAGTGGTTCCAGGATGCATCGCGTTCAGCTTGCCTCAGCGAAAATGGGCT
GGACTTTCTATTGGACAGGACATAGAAGTTGCCTTGTATTCGTTTGACAAAGCCAAGCAGTGCATCGGCA
CAATGACCATCGAGATCGACTTCCTGCAGAAGAAGAACATCGACTCCAACCCTTACGACACCGACAAGAT
GGCCGCCGAGTTCATCCAGCAGTTCAACAACCAGGCCTTCTCAGTGGGGCAGCAGCTTGTCTTTAGCTTC
AATGATAAGCTCTTTGGATTACTGGTGAAGGACATTGAAGCCATGGATCCCAGCATCCTGAAGGGAGAGC
CTGCATCAGGCAAAAGGCAGAAGATTGAGGTAGGCCTGGTTGTTGGAAACAGTCAAGTTGCATTTGAAAA
AGCAGAAAATTCGTCGCTCAATCTTATCGGCAAAGCTAAAACCAAGGAAAATCGCCAGTCCATCATCAAT
CCCGACTGGAACTTTGAGAAAATGGGGATCGGCGGTCTGGATAAGGAGTTCTCGGACATTTTCCGAAGAG
CATTTGCTTCTCGGGTGTTCCCCCCGGAGATCGTGGAACAGATGGGTTGCAAACATGTTAAAGGCATCCT
GTTATACGGACCCCCTGGTTGTGGTAAAACTCTCTTGGCTCGACAGATTGGCAAGATGCTGAATGCGAGA
GAGCCCAAGGTGGTCAATGGGCCAGAAATCCTTAACAAGTATGTGGGAGAATCAGAGGCTAACATTCGTA
AACTCTTTGCTGATGCCGAAGAGGAGCAAAGGAGGCTTGGTGCTAACAGTGGCTTGCACATCATCATCTT
TGATGAAATCGATGCCATCTGCAAGCAGAGAGGGAGCATGGCCGGCAGCACTGGAGTGCATGACACCGTG
GTCAACCAGCTGCTGTCCAAGATCGATGGCGTTGAGCAGCTGAACAACATCCTCGTTATCGGAATGACCA
ATAGACCAGATTTGATAGACGAAGCTCTCCTTCGACCCGGAAGACTGGAAGTTAAAATGGAGATAGGCTT
GCCAGATGAGAAGGGTCGACTCCAGATCCTTCATATCCACACAGCAAGGATGAGAGGGCACCAGTTACTG
TCTGCAGATGTGGACATCAAGGAGCTGGCTGTGGAGACTAAGAATTTCAGTGGCGCTGAGCTGGAGGGTC
TGGTGCGAGCAGCGCAGTCCACAGCCATGAACAGACATATCAAGGCCAGCACCAAAGTAGAAGTAGACAT
GGAGAAGGCAGAGAGCCTGCAGGTGACAAGAGGGGACTTCCTTGCTTCCCTGGAGAACGATATCAAACCA
GCATTTGGCACCAACCAAGAGGATTATGCAAGTTACATTATGAATGGGATCATCAAGTGGGGTGACCCCG
TGACCCGAGTCCTAGATGACGGAGAGCTGCTGGTCCAGCAAACGAAAAACAGTGACCGGACACCACTCGT
TAGTGTCCTCCTAGAAGGGCCTCCTCACAGCGGGAAGACTGCCCTGGCTGCAAAGATTGCCGAGGAATCC
AACTTCCCCTTCATCAAGATCTGCTCACCTGATAAGATGATTGGCTTCTCCGAGACAGCCAAGTGTCAGG
CCATGAAGAAGATCTTTGATGATGCTTACAAATCTCAGCTCAGCTGTGTGGTCGTGGATGACATTGAGAG
GCTGCTCGATTATGTCCCCATTGGTCCCCGGTTCTCCAACCTGGTATTGCAGGCTCTTCTTGTGTTACTG
AAAAAGGCGCCTCCTCAGGGCCGCAAGCTTCTTATCATCGGGACCACCAGCCGCAAAGATGTCCTGCAGG
AAATGGAGATGCTGAACGCCTTCAGCACCACCATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGCT
GGAAGCTCTGGAGCTTTTGGGCAACTTCAAAGATAAGGAACGCACCACAATTGCTCAGCAAGTCAAAGGG
AAGAAAGTCTGGATAGGAATCAAGAAGTTACTAATGTTGATTGAGATGTCCCTGCAGATGGATCCTGAGT
ACCGTGTGAGGAAATTCTTGGCCCTCATGAGAGAAGAAGGCGCTAGTCCCCTGGACTTTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210421 protein sequence
Red=Cloning site Green=Tags(s)

MAGRTMQAARCPTDELSLSNCAVVNEKDFQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWA
GLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSF
NDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIIN
PDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAR
EPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTV
VNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLL
SADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKP
AFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEES
NFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLL
KKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKG
KKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASPLDFD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008740
ORF Size 2232 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008740.4
RefSeq Size 3766 bp
RefSeq ORF 2235 bp
Locus ID 18195
UniProt ID P46460
Cytogenetics 11 67.54 cM
MW 82.6 kDa
Summary Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity).UniProtKB/Swiss-Prot Function
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"NM_008740" in other vectors (3)
SKU Description Size Price
MG210421 Nsf (tGFP-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf) 10 ug
$882.00
MR210421L3 Lenti ORF clone of Nsf (Myc-DDK-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf) 10 ug
$982.00
MR210421L4 Lenti ORF clone of Nsf (mGFP-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf) 10 ug
$982.00

Plasmid Map for MR210421

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210421
Circular map for MR210421

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