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Nup88 (BC032929) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210411
Nup88 (Myc-DDK-tagged) - Mouse nucleoporin 88 (cDNA clone MGC:41381 IMAGE:1379673)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $679.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nup88
Synonyms Nup84, 88kDa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210411 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCCGTGGGGCCCTTGGGCGATGGGGAGCTGTGGCAGAGCTGGCTTCCTAACCACGTCGTGT
TCTTGCGGCTCCGCGAGGGCGTAAGAAACCAGAGTCCAGCCGAAGCGGAGAAGCCAGCGGCTTCGACTTC
ACCCTCGTGCCCGTCTCTGCCGCCGCATTTGCCGACGAGAAACCTGGTCTTCGGCCTCGGAGGGGAACTG
TTTCTGTGGGACGCAGAAGGCAGCGCCTTCTTGGTGGTTCGCCTTCGAGGCCCCAGCGGTGGTGGCGTGG
AGCCTCCTCTCTCCCAGTATCAGAGATTACTCTGCATTAATCCACCCTTGTTTGAAATCCATCAAGTCTT
GTTGAGTCCAACCCAACATCATGTAGCACTTATCGGAAGTAAAGGACTTATGGCATTAGAATTACCTCAG
AGGTGGGGGAAGGACTCTGAATTTGAAGGTGGAAAAGCAACTGTGAATTGTAGCACCATCCCGATTGCTG
AGAGATTTTTCACCAGCTCTACCTCTCTGACTCTGAAGCATGCTGCATGGTATCCAAGTGAGATGCTGGA
TCCCCACATAGTGCTGTTGACATCAGACAACGTGATAAGAATTTATTCTCTCCGTGAGCCCCAGACACCC
ACTAAGGTGATTGTACTTTCAGAAGCAGAAGAGGAAAGTTTAATACTCAATAAAGGAAGAGCATATACAG
CGTCTCTAGGAGAGACTGCAGTGGCGTTTGACTTCGGGCCCCTGGTAACCGTCTCAAAGAATATATTTGA
ACAGAAAGACAGAGATGTGGTGGCGTATCCACTGTACATCCTGTATGAGAATGGGGAGACCTTCCTTACC
TACGTGAGCCTGTTACACAGCCCAGGGAATATTGGGAAGCTGTTGGGCCCACTGCCTATGCATCCTGCAG
CTGAAGATAACTACGGTTACGATGCCTGTGCTATACTCTGTTTGCCCTGTGTTCCAAATATCTTAGTAAT
TGCGACTGAGTCAGGAATGCTGTACCACTGTGTTGTACTAGAGGGAGAAGAAGAAGATGACCAAACGTTA
GAAAAGTCCTGGGATCCCAGGGCTGACTTCATCCCTTCTCTGTACGTGTTTGAGTGTGTTGAGTTAGAGC
TTGCCCTGAAGCTGGCATCTGGAGAGGACGATCCCTTTGCCTCTGACTTTTCCTGCCCAATTAAACTGCA
CAGAGATCCCAAGTGTCCTTCGAGATACCACTGCAGCCATGAAGCTGGCGTGCACAGTGTGGGGCTGACT
TGGATTCACAAACTGCACAAATTTCTTGGATCGGATGAAGAAGATAAGGATAGTTTACAAGAACTCACTG
CTGAGCAGAAATGCTTTGTGGAGCACATTCTTTGTACAAAGCCATTGCCGTGCAGGCAGCCAGCTCCAAT
TCGAGGATTCTGGATCGTCCCAGACATCCTGGGGCCCACAATGATCTGCATCACCAGTACCTATGAATGT
CTCATACGGCCTTTATTAAGTACAGTCCACCCAGCATCTCCTCCCCTGCTCTGTACCCAAGAAGATGCTG
AAGTTGCAGAGTCTCCACTGCGCATTCTGGCTGAAACTCCAGACTCCTTTGAGAAGCATATTAAAAGAAT
CTTGCAGCGTAGTGCTGCCAACCCAGCATTTCTCAAATCATCTGAAAAGGATTTGGCTCCCCCTCCCGAG
GAGTGTCTTCAGCTTATCAGCAGAGCCACCCAGGTGTTCCGAGAACAGTACATTCTCAAGCAGGACCTGG
CCAAGGAGGAGATTCAGCGGAGGGTCAAATTATTATGTGACCAAAAGAGGAAACAACTCGAAGATCTCAA
TTACTGTCGAGAGGAAAGGAAAAGTCTCCGGGAAATGGCTGAGCGCTTAGCTGACAAATATGAGGAAGCC
AAAGAAAAACAAGAAGATATCATGAACAGGATGAAAAAAGTGCTTCACAGTTTTCATGCTCAGCTCCCAG
TTCTCTCTGACAGTGAGAGAGACATGAAGAAAGAGTTACAGCTGATACCTGATCAACTGCGACATCTAGG
CAACGCCATCAAACAGGTTACTATGAAAAAAGATTATCAACAGCGGAAGATGGAAAAAGTGCTGAGTCCT
CAGAAACCCACCATTACTCTCAGTGCCTACCAGCGAAAGTGCATTCAGTCCATCCTGAAAGAAGAGGGTG
AACACATAAGGGAAATGGTGAAGCAAATCAATGACATCCGAAATCATGTCACCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210411 protein sequence
Red=Cloning site Green=Tags(s)

MAAAVGPLGDGELWQSWLPNHVVFLRLREGVRNQSPAEAEKPAASTSPSCPSLPPHLPTRNLVFGLGGEL
FLWDAEGSAFLVVRLRGPSGGGVEPPLSQYQRLLCINPPLFEIHQVLLSPTQHHVALIGSKGLMALELPQ
RWGKDSEFEGGKATVNCSTIPIAERFFTSSTSLTLKHAAWYPSEMLDPHIVLLTSDNVIRIYSLREPQTP
TKVIVLSEAEEESLILNKGRAYTASLGETAVAFDFGPLVTVSKNIFEQKDRDVVAYPLYILYENGETFLT
YVSLLHSPGNIGKLLGPLPMHPAAEDNYGYDACAILCLPCVPNILVIATESGMLYHCVVLEGEEEDDQTL
EKSWDPRADFIPSLYVFECVELELALKLASGEDDPFASDFSCPIKLHRDPKCPSRYHCSHEAGVHSVGLT
WIHKLHKFLGSDEEDKDSLQELTAEQKCFVEHILCTKPLPCRQPAPIRGFWIVPDILGPTMICITSTYEC
LIRPLLSTVHPASPPLLCTQEDAEVAESPLRILAETPDSFEKHIKRILQRSAANPAFLKSSEKDLAPPPE
ECLQLISRATQVFREQYILKQDLAKEEIQRRVKLLCDQKRKQLEDLNYCREERKSLREMAERLADKYEEA
KEKQEDIMNRMKKVLHSFHAQLPVLSDSERDMKKELQLIPDQLRHLGNAIKQVTMKKDYQQRKMEKVLSP
QKPTITLSAYQRKCIQSILKEEGEHIREMVKQINDIRNHVTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC032929
ORF Size 2226 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC032929, AAH32929
RefSeq Size 2418 bp
RefSeq ORF 2228 bp
Locus ID 19069
Cytogenetics 11 43.21 cM
MW 83.7 kDa
Summary Essential component of nuclear pore complex.UniProtKB/Swiss-Prot Function
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"BC032929" in other vectors (4)
SKU Description Size Price
MC206557 Nup88 (untagged) - Mouse nucleoporin 88 (cDNA clone MGC:41381 IMAGE:1379673), (10ug) 10 ug
$680.00
MG210411 Nup88 (tGFP-tagged) - Mouse nucleoporin 88 (cDNA clone MGC:41381 IMAGE:1379673) 10 ug
$879.00
MR210411L3 Lenti ORF clone of Nup88 (Myc-DDK-tagged) - Mouse nucleoporin 88 (cDNA clone MGC:41381 IMAGE:1379673) 10 ug
$979.00
MR210411L4 Lenti ORF clone of Nup88 (mGFP-tagged) - Mouse nucleoporin 88 (cDNA clone MGC:41381 IMAGE:1379673) 10 ug
$979.00

Plasmid Map for MR210411

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR210411
Circular map for MR210411

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