OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Plod3 (NM_011962) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR210405
Plod3 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $679.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Plod3 or NM_011962"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Plod3
Synonyms AI414586; LH3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210405 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGCAGGCCCGGAACCCCGGCTTTTGCTCCTGCTCCTGCTGCTGCTGCCGCCGCTGCCCCCCG
TAACTTCTGCCTCCGATCGACCCCGGGGCGCCAATGCTGTCAACCCAGACAAATTGCTGGTGATCACTGT
GGCTACGGCAGAGACAGAGGGGTACCGGCGTTTTCTGCAGTCTGCGGAGTTCTTTAACTACACTGTACGG
ACCCTGGGCCTGGGACAGGAGTGGCGAGGGGGTGATGTGGCTCGAACAGTTGGTGGAGGCCAGAAGGTCA
GGTGGCTCAAGAAGGAAATGGAGAAATATGCAGATCAGAAAGACATGATCATCATGTTTGTGGACAGCTA
CGACGTGATTCTGGCAAGCAGCCCGACAGAGCTGCTGAAGAAGTTTGTTCAGAGTGGCAGTCATCTGCTG
TTCTCTGCTGAGAGCTTCTGCTGGCCTGAGTGGGGGCTGGCAGAGCAGTACCCTGAGGTGGGCATGGGGA
AGCGCTTCCTCAACTCTGGTGGATTCATCGGCTTTGCTCCCACCATCCATCAGATTGTCCGCCAGTGGAA
CTACAAAGACGACGATGATGATCAATTGTTCTACACTCAACTGTACCTGGACCCAGGGCTGCGGGAGAAA
CTCAAACTGAGTCTGGACCATAAATCCCGGATCTTTCAGAACCTCAATGGAGCCTTAGATGAAGTGATCT
TAAAGTTTGACCAGAATCGTGTGCGCATCCGGAATGTGGCCTACGACACACTTCCTGTTGTGGTCCATGG
AAATGGTCCCACTAAGCTCCAGCTCAACTACCTGGGGAACTATGTCCCCAATGGCTGGACTCCCCAGGGA
GGCTGTGGATTCTGCAACCAGACCCTGAGGACACTCCCGGGGGGGCAGCCTCCCCCCCGGGTGCTTCTGG
CCGTGTTTGTGGAGCAGCCTACTCCCTTCCTGCCTCGGTTCCTGCAGCGCCTACTGCTCCTGGATTACCC
CCCGGACAGGATCTCTCTCTTCCTTCACAACAGCGAGGTGTACCACGAGCCTCATATTGCAGATGCCTGG
CCACAGCTCCAGGACCATTTTTCAGCTGTAAAGCTGGTGGGGCCAGAGGAGGCCCTGAGCGCAGGGGAGG
CCAGGGACATGGCCATGGACAGCTGTCGGCAGAACCCTGAGTGTGAGTTCTACTTTAGCCTGGATGCTGA
TGCCGTCCTTACGAACCCGGAGACCTTGCGTGTCTTGATTGAACAAAACAGGAAGGTAATAGCTCCCATG
CTTTCCCGCCATGGCAAGCTCTGGTCCAACTTCTGGGGTGCCCTGAGCCCCAATGAGTACTACGCCCGCT
CCGAAGACTACGTGGAACTGGTGCAGCGGAAGCGAGTGGGCGTGTGGAATGTACCCTACATATCTCAGGC
ATATGTGATCCGCGGGGAGACCCTGAGGACTGAACTGCCCCAAAAGGAGGTATTCTCCAGCAGCGACACA
GACCCAGATATGGCTTTCTGCAAGAGCGTCCGGGACAAGGGCATCTTCCTCCACCTCAGCAACCAGCACG
AGTTTGGCCGGCTGCTGGCTACTTCTCGCTATGACACAGACCACCTACACCCAGACCTCTGGCAGATCTT
TGACAACCCTGTGGACTGGAGAGAACAGTACATTCACGAGAATTACAGCCGGGCCCTGGATGGGGAAGGG
CTAGTGGAACAGCCATGCCCAGATGTGTACTGGTTCCCACTGCTGACCGAGCAGATGTGTGATGAGCTGG
TGGAGGAAATGGAACACTATGGCCAGTGGTCTGGAGGCCGGCACGAGGATTCCAGGTTGGCTGGAGGATA
CGAGAATGTTCCCACCGTTGATATCCACATGAAACAGGTGGGATACGAGGACCAGTGGCTTCAGCTGCTG
CGGACATATGTGGGGCCCATGACGGAGTACCTGTTCCCTGGCTACCACACCAAGACACGGGCAGTGATGA
ACTTCGTGGTCCGCTACCGGCCAGATGAGCAGCCCTCGCTTCGGCCACACCATGACTCGTCCACCTTCAC
TCTCAATGTCGCCCTCAACCACAAGGGTGTAGATTATGAGGGAGGCGGCTGCCGCTTCCTGCGTTACGAC
TGCAGAATCTCCTCTCCGAGGAAAGGCTGGGCCCTCCTGCACCCTGGCCGCCTCACACACTACCATGAGG
GGCTGCCCACCACCCGGGGTACTCGATACATCATGGTGTCCTTTGTTGACCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210405 protein sequence
Red=Cloning site Green=Tags(s)

MAAAGPEPRLLLLLLLLLPPLPPVTSASDRPRGANAVNPDKLLVITVATAETEGYRRFLQSAEFFNYTVR
TLGLGQEWRGGDVARTVGGGQKVRWLKKEMEKYADQKDMIIMFVDSYDVILASSPTELLKKFVQSGSHLL
FSAESFCWPEWGLAEQYPEVGMGKRFLNSGGFIGFAPTIHQIVRQWNYKDDDDDQLFYTQLYLDPGLREK
LKLSLDHKSRIFQNLNGALDEVILKFDQNRVRIRNVAYDTLPVVVHGNGPTKLQLNYLGNYVPNGWTPQG
GCGFCNQTLRTLPGGQPPPRVLLAVFVEQPTPFLPRFLQRLLLLDYPPDRISLFLHNSEVYHEPHIADAW
PQLQDHFSAVKLVGPEEALSAGEARDMAMDSCRQNPECEFYFSLDADAVLTNPETLRVLIEQNRKVIAPM
LSRHGKLWSNFWGALSPNEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGETLRTELPQKEVFSSSDT
DPDMAFCKSVRDKGIFLHLSNQHEFGRLLATSRYDTDHLHPDLWQIFDNPVDWREQYIHENYSRALDGEG
LVEQPCPDVYWFPLLTEQMCDELVEEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLL
RTYVGPMTEYLFPGYHTKTRAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGVDYEGGGCRFLRYD
CRISSPRKGWALLHPGRLTHYHEGLPTTRGTRYIMVSFVDP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011962
ORF Size 2223 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011962.3
RefSeq Size 3278 bp
RefSeq ORF 2226 bp
Locus ID 26433
UniProt ID Q9R0E1
Cytogenetics 5 76.09 cM
MW 84.9 kDa
Summary Multifunctional enzyme that catalyzes a series of post-translational modifications on Lys residues in procollagen (PubMed:16447251). Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:16447251). Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine (By similarity). Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:16447251, PubMed:16467571, PubMed:21220425). Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:25419660). Catalyzes hydroxylation and glycosylation of Lys residues in the ADIPOQ collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:23209641). Essential for normal biosynthesis and secretion of type IV collagens (PubMed:15377789, PubMed:16467571, PubMed:17873278). Essential for normal formation of basement membranes (PubMed:15377789, PubMed:16467571).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_011962" in other vectors (3)
SKU Description Size Price
MG210405 Plod3 (tGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) 10 ug
$879.00
MR210405L3 Lenti ORF clone of Plod3 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) 10 ug
$979.00
MR210405L4 Lenti ORF clone of Plod3 (mGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) 10 ug
$979.00

Plasmid Map for MR210405

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210405
Circular map for MR210405

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products