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Acap1 (NM_153788) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210394
Acap1 (Myc-DDK-tagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $679.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Acap1
Synonyms Centb1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210394 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGTCAAGCTAGATTTTGAAGAATGTCTCAAGGACTCACCCCGCTTCCGAGCCTCGATTGAACTGG
TGGAAACTGAAGTGTCAGAATTGGAGACCCGCCTGGAAAAGCTGCTAAAGCTGGGTTCCTGCCTCCTGGA
GAGCGGACAGCATTACCTTGCTGCCGGTCGTGCCTTCGTTGTTGGCATCTGTGACCTGGCTCGCCTGGGT
CCACCAGAGCCCATGATGGCGGAGTGTCTGGAAAAATTCACCGTGAGTCTGAACCACAAACTGGACAGCC
ATGCTGAGCTTCTTGATGCTACTCAACATACACTGCAGCAGCAAATCCAGACTCTGGTCAAGGAAGGTCT
CCGGGGCTTCCGAGAGGCTCGCAGGGATTTCTGGCGAGGGGCTGAGAACCTGGAGGCTGCTCTTACCCAC
AATGCAGAGGTCCCCAGGCGCCGTGTCCAAGAAGCAGAGGAGGCTGGAACTGCTTTGAGGACCGCTCGAG
CTGGGTACCGGAGTCGGGCATTAGATTATGCCCTGCAGGTCAATGTGATTGAGGATAAGAGGAAGTTCGA
CATCATGGAATTCGTACTGCGCTTGGTTGAGGCCCAGGCCACTTATTTCCAGCAGGGCCATGAAGAGCTA
AACAGGCTGGCTCAGTATCGGAAGGAATTGGGTACCCAGTTGCACAACCTGGTCTTGAACTCAGCTCGAC
AGAAGAGAGACATGGAACAGAGGCATGTGTTACTGAAGCAGAAGGAGCTGGGTGGCGAGGAGCCAGAGCC
AAGCCTAAAGGAGGGGCCTAGTGGCTTGGTAATGGAAGGACATCTTTTCAAACGTGCCAGCAATGCATTT
AAGACCTGGAGCAGACGCTGGTTCACCATTCAGAACAACCAGCTGGTTTACCAGAAGAAATATAAGGACC
CTGTAACCGTGGTGGTAGACGACCTTCGTCTCTGTACAGTGAAGCTCTGTCCAGACTCAGAAAGGCGTTT
CTGTTTCGAAGTAGTGTCCACCAGCAAGTCCTGTTTCCTCCAGGCTGACTCTGAGCGTCTCCTGCAGCTG
TGGGTCAGTGCTGTCCAGAGCAGCATTGCCTCAGCCTTCAGCCAGGCTCACCTGGAGAACAGCCCTCGGG
GGCCAGGCCAGGTCTCAGGATACCATGCCCCGGGCTCTGCTGCAACTCTGGCCTGTGGCGGGGCAGCCAG
AGGAAGGGAGTCTGGAGGAGTTGGTCAGGTAGCGGCCCAGGTACAAAGCGTGGATGGAAATGCGCAATGC
TGCGACTGCAGGGAGCCAGCCCCAGAGTGGGCCAGCATCAACCTTGGCGTCACTCTCTGCATTCAGTGTT
CTGGCATCCACAGGAGCCTCGGAGTTCATTTCTCCAAAGTCCGCTCTCTGACCCTTGACTCCTGGGAACC
AGAACTGGTGAAGCTTATGTGCGAGCTGGGGAATGTCATCATCAACCAGATCTATGAGGCCCGGGTGGAG
GCCATGGCGGTGAAGAAGCCAGGGCCCAGCTGCTCCCGGCAGGAAAAGGAAGCCTGGATTCATGCCAAGT
ATGTGGAGAAAAAGTTCCTGACCAAACTTCCTGAAATCCGTGGGCGAAGAGGTGGCAGGGGACCCCCAAG
AGGACATCCTCCTGTGCCCCCAAAGCCTCCCATCAGGCCACACTCGGGCATTGTCAGATCCAAATCAGAG
TGCCCGTCTGATGACATGGGAAGCCTGCACCCCGGGGCCCTGCTGTTTCAAGCTGCTGGACATCCCCCAT
CTCTTCCTACCATGGCTGATGCCCTGGCCCATGGAGCTGATGTCAACTGGGTCAATGTGGGCCAGGGGAA
TGCCACACCTCTGATCCGGGCCACAGCTGCTAACTCTCTTCTGGCCTGCGAGTTTCTCCTCCAAAATGGG
GCGAATGTGAACCAAGCAGACAGTGCCGGCCGGGGTCCTCTCCACCATGCCACCATTCTTGGTCACACAG
GGCTCGCTTGCCTGTTCCTGAAACGGGGTGCTGATCTGGGGGCTCGAGACACAGAAGGCAGGGACCCTCT
GACTATCGCAATGGAAACCACTAATGCTGACATCGTAACCCTGCTACGATTGGCAAAGATGAGGGAGGCT
GAAGCGGCCCAGGGCCAGGCAGGAGACGAGACGTATCTCGACATATTCCGAGACTTCTCCCTCATGGCGT
CGGACGACCCAGAGAAACTGAGCCGTCGCAGTCACGACCTCCATACTCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210394 protein sequence
Red=Cloning site Green=Tags(s)

MTVKLDFEECLKDSPRFRASIELVETEVSELETRLEKLLKLGSCLLESGQHYLAAGRAFVVGICDLARLG
PPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAENLEAALTH
NAEVPRRRVQEAEEAGTALRTARAGYRSRALDYALQVNVIEDKRKFDIMEFVLRLVEAQATYFQQGHEEL
NRLAQYRKELGTQLHNLVLNSARQKRDMEQRHVLLKQKELGGEEPEPSLKEGPSGLVMEGHLFKRASNAF
KTWSRRWFTIQNNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCFLQADSERLLQL
WVSAVQSSIASAFSQAHLENSPRGPGQVSGYHAPGSAATLACGGAARGRESGGVGQVAAQVQSVDGNAQC
CDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVE
AMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGPPRGHPPVPPKPPIRPHSGIVRSKSE
CPSDDMGSLHPGALLFQAAGHPPSLPTMADALAHGADVNWVNVGQGNATPLIRATAANSLLACEFLLQNG
ANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDTEGRDPLTIAMETTNADIVTLLRLAKMREA
EAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153788
ORF Size 2220 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153788.2
RefSeq Size 2482 bp
RefSeq ORF 2223 bp
Locus ID 216859
UniProt ID Q8K2H4
Cytogenetics 11 B3
MW 81.7 kDa
Summary GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration (By similarity).UniProtKB/Swiss-Prot Function
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"NM_153788" in other vectors (4)
SKU Description Size Price
MC202886 Acap1 (untagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1), (10ug) 10 ug
$679.00
MG210394 Acap1 (tGFP-tagged) - Mouse centaurin, beta 1 (Centb1) 10 ug
$879.00
MR210394L3 Lenti ORF clone of Acap1 (Myc-DDK-tagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1) 10 ug
$979.00
MR210394L4 Lenti ORF clone of Acap1 (mGFP-tagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1) 10 ug
$979.00

Plasmid Map for MR210394

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210394
Circular map for MR210394

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