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Cop1 (NM_011931) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210351
Rfwd2 (Myc-DDK-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $671.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cop1
Synonyms AI316802; C80879; Cop1; Rfwd2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210351 representing NM_011931
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGGTAGCCGCCAGGCCGGCTCGGGTTCCGCTGGGACGAGCCCCGGCTCCTCGGCCGCCTCCTCGG
TGACTTCCGCCTCCTCGTCCTTATCCTCGTCTCCGTCGCCGCCGTCCGTGGCGGCCTCGGCGGCGACGCT
GGTGTCCGGCGGCGTGGCTCCGGCCGCGGGCTCCGGCGGCCTCGGCGGCCCGGGGCGGCCTGTGCTGGTG
GCGGCCGCGGTGTCTGGCAGCGCGAGCGCCGGCGGGGCGGTGTCCGCGGGCCAGTCCCGGCTCAGCTGCG
CGGCCAGGCCCAGCGCCGGCGTTGGAGGGAGCAGCTCCAGCCTCGGCAGTAGCAGTAGGAAGCGACCTCT
GCTCGTCCCGCTTTGTAACGGGCTCCTCAACTCCTACGAGGACAAAAGCAACGACTTCGTCTGTCCCATC
TGCTTTGATATGATTGAGGAGGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATTCATC
AGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAATTATGTTGTGGACAATATTGACCATCTCTATCC
TAATTTCTTGGTGAATGAACTCATTCTCAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGAC
CACTCAGTGAGTAGCACCAATGGGCATAGGTGGCAAATATTTCAAGATCTACTAGGAACTGATCAAGATA
ACCTTGATTTGGCCAATGTCAACCTCATGTTGGAATTATTAGTGCAGAAGAAGAAACAACTGGAAGCAGA
ATCACATGCAGCTCAGCTACAGATCCTTATGGAATTCCTCAAGGTTGCAAGGAGAAATAAGAGAGAGCAA
TTGGAGCAGATCCAGAAGGAACTAAGTGTTTTGGAAGAGGATATTAAAAGAGTGGAAGAAATGAGTGGCC
TCTACTCTCCTGTCAGTGAAGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGCAT
TATTGATTCTACAGAATACAGCCAACCTCCAGGTTTCAGTGGGACCTCTCAGACAAAGAAACAGCCTTGG
TATAACAGCACATTAGCATCAAGACGAAAGCGACTCACTGCTCATTTTGAAGACTTAGAGCAGTGTTATT
TTTCTACAAGGATGTCTCGTATCTCAGATGACAGTAGAACTGCAAGCCAGTTAGATGAATTTCAAGAATG
CTTGTCCAAGTTTACTCGATACAACTCAGTAAGACCGTTGGCCACATTGTCTTATGCTAGTGATCTCTAT
AATGGTTCCAGCATAGTTTCTAGTATTGAGTTTGACCGGGATTGTGACTATTTTGCAATTGCTGGAGTTA
CAAAGAAGATTAAAGTCTATGAGTATGGCACAGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAA
TGAAATGACCTGCAATTCCAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGCTAGCCAGC
AGTGATTATGAAGGCACTGTTATACTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGC
ATGAAAAAAGGTGTTGGAGTGTTGACTTTAATCTGATGGATCCTAAACTTCTGGCTTCAGGTTCTGATGA
TGCAAAAGTGAAGCTGTGGTCTACCAATTTAGACAATTCTGTGGCGAGCATTGAGGCAAAGGCTAACGTG
TGCTGTGTGAAGTTCAGCCCCTCCTCCAGGTACCATCTGGCTTTCGGCTGTGCAGATCACTGTGTCCACT
ACTACGACCTTCGTAACACTAAACAGCCAATAATGGTATTCAAGGGACACCGAAAAGCAGTGTCTTATGC
CAAGTTTGTAAGTGGTGAAGAAATTGTCTCAGCCTCAACAGACAGCCAACTAAAGCTGTGGAATGTGGGA
AAACCATACTGTCTACGTTCCTTCAAGGGTCATATTAATGAAAAAAACTTTGTAGGCCTTGCTTCCAATG
GAGATTATATAGCGTGTGGAAGTGAGAACAACTCCCTCTACCTGTATTATAAAGGACTTTCTAAAACTTT
GCTAACTTTTAAGTTTGATACCGTTAAGAGTGTATTAGACAAAGATCGGAAAGAAGATGACACAAATGAA
TTTGTCAGTGCTGTGTGTTGGCGGGCACTATCAGACGGGGAGTCCAATGTGCTGATTGCTGCTAACAGCC
AGGGTACAATTAAGGTGCTAGAATTGGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210351 representing NM_011931
Red=Cloning site Green=Tags(s)

MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAASAATLVSGGVAPAAGSGGLGGPGRPVLV
AAAVSGSASAGGAVSAGQSRLSCAARPSAGVGGSSSSLGSSSRKRPLLVPLCNGLLNSYEDKSNDFVCPI
CFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLD
HSVSSTNGHRWQIFQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQ
LEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGTSQTKKQPW
YNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLY
NGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLAS
SDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANV
CCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVG
KPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNE
FVSAVCWRALSDGESNVLIAANSQGTIKVLELV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011931
ORF Size 2199 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011931.4
RefSeq Size 5056 bp
RefSeq ORF 2202 bp
Locus ID 26374
UniProt ID Q9R1A8
Cytogenetics 1 H1
MW 80.9 kDa
Summary E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif.UniProtKB/Swiss-Prot Function
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"NM_011931" in other vectors (4)
SKU Description Size Price
MC221164 Rfwd2 (untagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2), (10ug) 10 ug
$739.00
MG210351 Rfwd2 (tGFP-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2) 10 ug
$871.00
MR210351L3 Lenti ORF clone of Rfwd2 (Myc-DDK-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2) 10 ug
$971.00
MR210351L4 Lenti ORF clone of Rfwd2 (mGFP-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2) 10 ug
$971.00

Plasmid Map for MR210351

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210351
Circular map for MR210351

file-alt Citations

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