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Nadsyn1 (NM_030221) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210307
Nadsyn1 (Myc-DDK-tagged) - Mouse NAD synthetase 1 (Nadsyn1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $665.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nadsyn1
Synonyms 9130012B15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210307 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCGGAAAGTGACCGTGGCCACCTGTGCACTCAATCAGTGGGCCCTGGACTTTGAGGGCAATTTCC
AGAGGATTTTAAAGAGTATTCAAATTGCCAAAGGCAAAGGTGCAAGATACAGGCTTGGACCAGAACTAGA
AATATGCGGCTATGGATGTTGGGATCATTATCACGAATCAGACACTCTCCTGCATTCGCTCCAAGTTCTG
GCTGCTCTTCTGGACTCTCCGGTCACTCAGGACATCATCTGTGATGTGGGGATGCCTATAATGCACCGGA
ATGTTCGCTACAACTGCAGAGTCATCTTTCTCAACAGGAAGATCCTGCTCATCAGACCCAAGATGGCCTT
GGCCAACGAAGGCAACTACCGGGAACTGCGCTGGTTTACCCCCTGGACCAGGAGCCGACAAACTGAGGAA
TATGTCCTCCCTCGGATGTTACAGGACCTGACAAAGCAGAAAACTGTGCCCTTTGGAGATGTGGTACTGG
CCACCCAGGACACCTGTGTCGGGAGTGAGATCTGTGAGGAGCTCTGGACACCACGCAGCCCCCACGTCGA
CATGGGCCTGGATGGTGTGGAGATCATTACCAATGCCTCGGGCAGCCACCACGTGCTCCGCAAAGCCCAT
ACCAGAGTGGATCTGGTAACCATGGCCACCTCCAAGAATGGTGGCATCTACCTGCTGGCCAACCAGAAGG
GCTGCGACGGTGACCGTCTCTACTACGATGGCTGTGCCATGATTGCTATGAACGGGAGCATCTTTGCCCA
GGGCACTCAGTTCTCATTGGATGATGTGGAGGTTCTCACTGCCACCCTGGACCTGGAGGACGTTAGGAGC
TACAGGGCAGAGATCTCATCTCGGAACCTGGAGGCAACCAGGGTGAGCCCATATCCCCGGGTGACGGTGG
ACTTTGCCCTTTCGGTCAGTGAAGACTTGTTGGAGCCAGTGTCAGAGCCTATAGAATGGACATACCACAG
GCCTGAGGAGGAGATAAGCCTCGGCCCTGCCTGCTGGCTCTGGGACTTCCTTCGACGGAGCAAACAGGCT
GGGTTTTTCCTGCCCCTGAGTGGTGGTGTGGACAGTGCAGCCAGCGCCTGTATCGTCTACTCCATGTGCT
GCCTGGTCTGCGATGCTGTGAAGAGCGGAAATCAGCAAGTGCTGACAGACGTCCAGAACCTGGTGGATGA
GAGCAGCTACACCCCTCAGGATCCCCGGGAGCTCTGCGGACGGCTGCTCACTACTTGTTACATGGCCAGC
GAAAACTCCTCCCAGGAGACCCACAGCAGAGCCACAAAACTGGCTCAGCTGATTGGAAGCTACCACATCA
ATCTGAGCATCGACACTGCTGTGAAGGCTGTCTTGGGCATCTTCAGCCTGGTGACCGGGAAGTTGCCTCG
GTTCTCAGCCCACGGAGGGAGCAGCAGGGAGAACCTGGCCTTGCAGAATGTGCAGGCTCGGATTAGGATG
GTCCTTGCCTACCTGTTTGCTCAGCTGAGCCTCTGGTCTCGGGGTGCTCGAGGAAGCCTTCTTGTGCTTG
GATCTGCCAATGTGGATGAGAGTCTCCTCGGCTACCTGACCAAGTACGACTGCTCCAGTGCGGACATCAA
CCCCATAGGCGGCATCAGCAAGACGGACCTGAGAGCCTTTGTCCAGTTTTGCGCAGAGCGCTTCCAGCTT
CCTGTTCTGCAGACCATCCTGTCAGCACCGGCCACTGCAGAGCTGGAGCCCTTAGCTGATGGGCAGGTGT
CTCAGATGGATGAGGAAGACATGGGGATGACGTACGCAGAACTCTCCATCTTCGGCAGGCTCCGGAAGGT
CGCCAAGGCAGGCCCCTACAGCATGTTCTGCAAACTGCTCAACATGTGGAGAGACAGCTATACACCAACA
CAGGTAGCTGAGAAGGTGAAGCTATTTTTCTCAAAGTACTCCATGAACAGGCACAAGATGACAACCCTCA
CACCAGCATATCATGCTGAAAACTACAGTCCTGACGACAACAGGTTTGACCTGAGACCCTTTCTGTACAA
CACGAGATGGCCCTGGCAGTTCCTCTGCATTGACAACCAGGTTCTACAGCTTGAGAGGAAAGCATCGCAG
ACCCGGGAAGAACAGGTCCTGGAGCACCTCAAAGAGCCATCACCTATATGGAAACAGCTCCTCCCCAAGG
ACCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210307 protein sequence
Red=Cloning site Green=Tags(s)

MGRKVTVATCALNQWALDFEGNFQRILKSIQIAKGKGARYRLGPELEICGYGCWDHYHESDTLLHSLQVL
AALLDSPVTQDIICDVGMPIMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWTRSRQTEE
YVLPRMLQDLTKQKTVPFGDVVLATQDTCVGSEICEELWTPRSPHVDMGLDGVEIITNASGSHHVLRKAH
TRVDLVTMATSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSIFAQGTQFSLDDVEVLTATLDLEDVRS
YRAEISSRNLEATRVSPYPRVTVDFALSVSEDLLEPVSEPIEWTYHRPEEEISLGPACWLWDFLRRSKQA
GFFLPLSGGVDSAASACIVYSMCCLVCDAVKSGNQQVLTDVQNLVDESSYTPQDPRELCGRLLTTCYMAS
ENSSQETHSRATKLAQLIGSYHINLSIDTAVKAVLGIFSLVTGKLPRFSAHGGSSRENLALQNVQARIRM
VLAYLFAQLSLWSRGARGSLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCAERFQL
PVLQTILSAPATAELEPLADGQVSQMDEEDMGMTYAELSIFGRLRKVAKAGPYSMFCKLLNMWRDSYTPT
QVAEKVKLFFSKYSMNRHKMTTLTPAYHAENYSPDDNRFDLRPFLYNTRWPWQFLCIDNQVLQLERKASQ
TREEQVLEHLKEPSPIWKQLLPKDP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030221
ORF Size 2175 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030221.1, NM_030221.2, NP_084497.1
RefSeq Size 2632 bp
RefSeq ORF 2178 bp
Locus ID 78914
UniProt ID Q711T7
Cytogenetics 7 F5
MW 81.6 kDa
Summary Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.UniProtKB/Swiss-Prot Function
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"NM_030221" in other vectors (4)
SKU Description Size Price
MC203062 Nadsyn1 (untagged) - Mouse NAD synthetase 1 (Nadsyn1), (10ug) 10 ug
$665.00
MG210307 Nadsyn1 (tGFP-tagged) - Mouse NAD synthetase 1 (Nadsyn1) 10 ug
$865.00
MR210307L3 Lenti ORF clone of Nadsyn1 (Myc-DDK-tagged) - Mouse NAD synthetase 1 (Nadsyn1) 10 ug
$965.00
MR210307L4 Lenti ORF clone of Nadsyn1 (mGFP-tagged) - Mouse NAD synthetase 1 (Nadsyn1) 10 ug
$965.00

Plasmid Map for MR210307

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210307
Circular map for MR210307

file-alt Citations

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