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Bbs2 (NM_026116) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210279
Bbs2 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $660.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bbs2
Synonyms 2410125H22Rik; AI447581
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210279 representing NM_026116
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGCCTGTGTTCACTCTGAAACTGCGCCACAAAATCAGCCCCCGCATGGTGGCCATAGGGCGCT
ACGACGGGACTCACCCGTGCTTGGCTGCCGCCACCCAAGCGGGCAAGGTTTTCATTCATAACCCTCACAC
GCGGAGCCAGCATTTCAGTGCTTCCAGAGTGTTCCAGAGCCCTCTGGAGTCTGATGTCTCTCTGCTCAAC
ATTAACCAGACGGTCAGCTGCCTGGGTTCGGGAGTCTTGAACCCTGAGCTTGGCTATGATACTCTTTTAG
TGGGGACACAGACCAGTCTTTTGGCTTACGACATCTACAATAATTCAGATTTGTTCTACAGAGAGGTCTC
AGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGA
AACTGTGCTCTGCAGGGTTTCGATCACGAAGGAAATGATCTCTTTTGGACGGTTACTGGAGACAATGTGC
ATTCCTTGGCTCTGTGTGACTTTGACGGTGATGGGAAGACAGAGCTTCTTGTTGGATCTGAAGATTTTGA
CATTCGAGTTTTTAAAGAAGATGAGATTGTGGCAGAAATGACAGAGACAGAGATAGTCACCTCTCTGTGT
CCTATGTATGGCAGTCGGTTTGGTTACGCCCTTTCTAATGGCACAGTTGGAGTTTATGACAAAACAGCCC
GGTACTGGAGAATTAAATCCAAAAACCATGCCATGAGCATCCATGCTTTTGACATTAATTCTGATGGAGT
GTGTGAACTGATAACCGGGTGGTCTAATGGGAAGGTTGATGCTCGCAGTGACCGGACTGGAGAGGTCATA
TTTAAAGACAACTTCTCTTCTGCTGTTGCTGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACGTAC
AGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAA
CCTCCTGGACACAAGTGTGGAGCAGGACCTGATCCGAGAGCTGAGTCAGAAAAAGCAGAACCTGTTGCTA
GAACTTCGCAACTATGAGGAGAGCACCAAGGCAGAACTGAGCAGTCCCCTGAACGAGGCCGATGGGCAGA
AAGGCATAATCCCAGCCAATACCAGGCTCCACACAGCGCTCTCAGTCAATATGGGGAATGACTTACAAGA
TGCGCATGCGGAATTAGGCATTTCCACTTCCAATGATACTATCATCCGAGCAGTACTAATTTTTGCAGAG
GGAATTTTTGTGGGTGAAAGCCATGTGGTCCACCCCAGCATTCACAACCTTTCCAGCTCCCTCCGGGTAC
CAATTACACCTCCCAAAGATGTCCCTGTGGATCTGCACCTGAAAACCTTCGTGGGTTACAGAAGCAGCAC
CCAGTTCCATGTGTTTGAACTGACAAGACAGCTGCCCCGATTCACCATGTATGCATTGACAAGCCCAGAT
GCAGCTAGCGAGCCTGTCAGCTACGTGAACTTCAGTGTTGCCGAGCGCACACAGAGGATGGTCACGTGGC
TCAACCAGAACTTTCTGTTGCCAGAAGACAGTAACGTTCAGAATTCCCCGTTTCACGTCTGTTTCACATC
GTTACGCAATGGTGGCCAGCTCTACATAAAAATGAAACAAAGTGGTGAGATCACTGTGAATACTGACGAT
ATTGATCTAGCTGGTGACATCATCCAGTCGATCGCGTCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAG
CAGATTTCCCTGTGTACTTCGAGGAACTACGGAAAGTGCTGGTGAAGGTGGATGAGTATCATTCAGTGCA
TCAAAAGCTCAGCGCCGACATGGCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCGGAGGAT
GCTCGGCTGATGAGGGACATGAAAACAATGAAGAGTCGTTACATGGAGCTCTATGACCTTAATAAAGATT
TGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCA
AGCAATCCAAAGAGCAGGCCGTCTGCGCGTTGGAAAGCCAAAGAACCAGGTGATCAGTGCTTGTCGGGAT
GCGATTCGAAGCAACAACATCAACACCCTCTTCAGAATCATGCGAGTGGGCACTGCTCCTTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210279 representing NM_026116
Red=Cloning site Green=Tags(s)

MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQAGKVFIHNPHTRSQHFSASRVFQSPLESDVSLLN
INQTVSCLGSGVLNPELGYDTLLVGTQTSLLAYDIYNNSDLFYREVSDGANAIVLGTLGDIAPPLAIIGG
NCALQGFDHEGNDLFWTVTGDNVHSLALCDFDGDGKTELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLC
PMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVI
FKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLL
ELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAE
GIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPD
AASEPVSYVNFSVAERTQRMVTWLNQNFLLPEDSNVQNSPFHVCFTSLRNGGQLYIKMKQSGEITVNTDD
IDLAGDIIQSIASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADNSNLIRSLLVRAED
ARLMRDMKTMKSRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVISACRD
AIRSNNINTLFRIMRVGTAPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026116
ORF Size 2163 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026116.3
RefSeq Size 2972 bp
RefSeq ORF 2166 bp
Locus ID 67378
UniProt ID Q9CWF6
Cytogenetics 8 C5
MW 80.4 kDa
Summary The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026116" in other vectors (4)
SKU Description Size Price
MC204609 Bbs2 (untagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2), (10ug) 10 ug
$661.00
MG210279 Bbs2 (tGFP-tagged) - Mouse Bardet-Biedl syndrome 2 homolog (human) (Bbs2) 10 ug
$860.00
MR210279L3 Lenti ORF clone of Bbs2 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2) 10 ug
$960.00
MR210279L4 Lenti ORF clone of Bbs2 (mGFP-tagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2) 10 ug
$960.00

Plasmid Map for MR210279

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210279
Circular map for MR210279

file-alt Citations

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