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Mpo (NM_010824) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210262
Mpo (Myc-DDK-tagged) - Mouse myeloperoxidase (Mpo), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $658.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mpo
Synonyms mKIAA4033
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210262 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCTACTCTTGGCCTTGGCAGGCCTCCTGGCCCCTCTGGCCATGCTTCAGACCTCCAATGGTGCCA
CCCCAGCTCTCCTGGGGGAAGTGGAGAATTCAGTTGTGCTGAGCTGTATGGAAGAGGCCAAGCAGCTAGT
GGACAGAGCCTACAAGGAGCGGAGAGAAAGCATCAAGCGGAGCCTCCAAAGCGGCTCTGCCAGCCCCACG
GAGCTCCTGTTTTACTTCAAGCAGCCGGTGGCGGGCACCAGAACAGCTGTGAGGGCCGCTGATTATCTAC
ATGTGGCCCTAGACCTGCTGAAGAGGAAGCTGCAGCCCCTGTGGCCAAGGCCTTTCAATGTTACAGATGT
GTTGACACCTGCTCAGCTGAATCTGTTGTCCGTGTCAAGTGGCTGTGCCTATCAGGACGTGAGGGTGACA
TGCCCACCGAATGACAAGTATCGCACCATCACTGGACACTGCAACAACAGACGAAGCCCCACTCTGGGGG
CCTCCAACCGTGCCTTTGTACGCTGGTTGCCTGCAGAGTATGAAGATGGCGTCTCCATGCCCTTTGGCTG
GACGCCTGGAGTCAATCGCAATGGCTTCAAGGTGCCCCTGGCTCGCCAGGTCTCCAATGCCGTCGTGCGC
TTCCCCAACGATCAGCTGACCAAGGACCAGGAGCGTGCACTCATGTTCATGCAGTGGGGACAGTTTCTGG
ATCATGATATCACCTTGACTCCAGAGCCAGCTACCCGGTTCTCCTTCTTCACTGGCCTCAACTGCGAGAC
CAGCTGCCTGCAGCAGCCACCCTGCTTCCCCCTCAAGATCCCACCCAATGACCCTCGAATCAAGAACCAA
AAGGACTGCATCCCCTTCTTCCGCTCCTGCCCGGCATGCACCAGGAACAACATCACCATTCGCAACCAGA
TCAACGCGCTCACTTCCTTCGTGGACGCCAGCGGGGTGTACGGCAGCGAGGACCCCCTAGCCAGAAAGCT
GCGCAACCTCACCAACCAGCTGGGGCTGCTGGCTATCAATACACGCTTCCAAGACAATGGCAGGGCCCTG
ATGCCCTTTGACAGCCTGCACGATGACCCCTGCCTCCTCACCAACCGCTCCGCCCGCATTCCTTGTTTTC
TGGCAGGGGACATGCGCTCCAGCGAGATGCCGGAGCTCACCTCCATGCACACCCTCTTTGTTCGAGAGCA
TAACCGGCTGGCCACACAGCTCAAGCGCCTGAATCCTCGATGGAATGGGGAGAAGCTCTACCAGGAGGCC
CGGAAGATTGTAGGGGCCATGGTCCAGATCATCACATACCGGGACTACCTGCCCTTGGTGTTGGGGCCAG
CAGCCATGAAGAAGTACCTACCCCAGTACCGATCTTACAACGACTCAGTAGACCCTCGAATCGCCAATGT
CTTCACCAACGCTTTCCGTTATGGCCACACCCTCATCCAACCCTTCATGTTCCGCCTGAACAATCAGTAC
CGGCCCACAGGGCCCAACCCCCGAGTCCCACTCAGCAAGGTCTTTTTTGCCAGCTGGAGAGTCGTGTTGG
AAGGTGGCATTGACCCCATCCTCCGAGGCCTCATGGCCACTCCAGCCAAACTGAATCGCCAGAATCAAAT
TGTGGTGGATGAGATCCGGGAGCGACTATTTGAGCAAGTCATGAGGATAGGACTGGATTTGCCTGCTCTT
AACATGCAGCGCAGCCGGGATCACGGCCTCCCAGGATACAATGCCTGGAGACGCTTTTGTGGGCTTCCAC
AGCCCAGCACAGTGGGTGAGCTCGGCACGGTGCTGAAGAACCTGGAGTTGGCACGGAAGCTGATGGCACA
ATATGGCACGCCCAACAACATTGACATCTGGATGGGCGGTGTGTCCGAGCCCCTGGAGCCCAATGGCCGT
GTAGGCCAGCTCCTTGCCTGCCTCATTGGCACTCAGTTTAGGAAGCTACGTGATGGTGATCGGTTTTGGT
GGGAGAACCCAGGCGTGTTCAGTAAACAGCAGAGACAGGCCTTGGCCAGCATCTCCTTGCCCCGTATCAT
CTGTGACAACACTGGCATCACCACTGTGTCGAAGAACAACATCTTCATGTCCAACACATACCCCCGAGAC
TTTGTCAGCTGTAACACTCTTCCTAAACTGAACCTGACTTCCTGGAAGGAGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210262 protein sequence
Red=Cloning site Green=Tags(s)

MKLLLALAGLLAPLAMLQTSNGATPALLGEVENSVVLSCMEEAKQLVDRAYKERRESIKRSLQSGSASPT
ELLFYFKQPVAGTRTAVRAADYLHVALDLLKRKLQPLWPRPFNVTDVLTPAQLNLLSVSSGCAYQDVRVT
CPPNDKYRTITGHCNNRRSPTLGASNRAFVRWLPAEYEDGVSMPFGWTPGVNRNGFKVPLARQVSNAVVR
FPNDQLTKDQERALMFMQWGQFLDHDITLTPEPATRFSFFTGLNCETSCLQQPPCFPLKIPPNDPRIKNQ
KDCIPFFRSCPACTRNNITIRNQINALTSFVDASGVYGSEDPLARKLRNLTNQLGLLAINTRFQDNGRAL
MPFDSLHDDPCLLTNRSARIPCFLAGDMRSSEMPELTSMHTLFVREHNRLATQLKRLNPRWNGEKLYQEA
RKIVGAMVQIITYRDYLPLVLGPAAMKKYLPQYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLNNQY
RPTGPNPRVPLSKVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIVVDEIRERLFEQVMRIGLDLPAL
NMQRSRDHGLPGYNAWRRFCGLPQPSTVGELGTVLKNLELARKLMAQYGTPNNIDIWMGGVSEPLEPNGR
VGQLLACLIGTQFRKLRDGDRFWWENPGVFSKQQRQALASISLPRIICDNTGITTVSKNNIFMSNTYPRD
FVSCNTLPKLNLTSWKET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010824
ORF Size 2154 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010824.2
RefSeq Size 2570 bp
RefSeq ORF 2157 bp
Locus ID 17523
UniProt ID P11247
Cytogenetics 11 C
MW 81.2 kDa
Summary Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.UniProtKB/Swiss-Prot Function
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Documents
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"NM_010824" in other vectors (4)
SKU Description Size Price
MC201863 Mpo (untagged) - Mouse myeloperoxidase (Mpo), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$658.00
MG210262 Mpo (tGFP-tagged) - Mouse myeloperoxidase (Mpo) 10 ug
$789.00 $858.00
MR210262L3 Lenti ORF clone of Mpo (Myc-DDK-tagged) - Mouse myeloperoxidase (Mpo), nuclear gene encoding mitochondrial protein 10 ug
$958.00
MR210262L4 Lenti ORF clone of Mpo (mGFP-tagged) - Mouse myeloperoxidase (Mpo), nuclear gene encoding mitochondrial protein 10 ug
$958.00

Plasmid Map for MR210262

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210262
Circular map for MR210262

file-alt Citations

Myeloperoxidase controls bone turnover by suppressing osteoclast differentiation through modulating reactive oxygen species level
Zhao, Lin, Li et al
J Bone Miner Res (2020)
Cited by 44
AI Summary: OriGene's pCMV6-Mpo-FLAG and negative control cloning vector were used to modulate MPO expression in vitro, enabling the authors to investigate how changes in MPO levels affect osteoclast differentiation and bone turnover.
Snippet: siRNA were generated by RIBOBio (Guangzhou, China). pCMV6-Mpo-FLAG and negative control cloning vector were purchased from OriGene (Rockville, MD, USA). RAW264.7 cells and BMMs were seeded into 96-well plates with 3000 cells/well or 5000
Showing 1-1 of 1 papers.
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