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Hook3 (NM_207659) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210257
Hook3 (Myc-DDK-tagged) - Mouse hook homolog 3 (Drosophila) (Hook3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $660.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hook3
Synonyms 5830454D03Rik; AI317159; E330005F07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210257 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTAACGTGGAGTCTGTGGAGCGGGTGGAGCTGTGCGAGAGCCTGCTCACTTGGATCCAGACCTTTA
ATGTGGATGCACCGTGCCAGACCGCGGAGGACTTAACGAACGGCGTTGTCATGTCCCAGGTTCTTCAGAA
GATAGATCCTGTATATTTCGACGATAATTGGCTAAACAGAATCAAAACTGAAGTAGGAGACAATTGGAGG
TTAAAGATCAGCAATTTAAAGAAAATTTTAAAAGGCATCTTGGATTACAATCATGAGATTTTAGGACAGC
AGATTAATGATTTCACCCTTCCTGACGTGAACCTCATTGGGGAGCATTCGGATGCAGCAGAGCTCGGAAG
GATGCTGCAGCTCATTCTAGGCTGTGCTGTGAACTGTGAGCAGAAGCAAGAGTACATTCAAGCCATTATG
ATGATGGAGGAATCTGTTCAGCATGTCGTCATGACAGCCATTCAAGAGCTAATGAGTAAAGAGTCTCCTG
TCTCTGCTGGACATGATGCCTATGTTGATCTTGATCGCCAGTTGAAGAAAACTACCGAAGAACTAAATGA
AGCTTTGTCAGAAAAGGAGGAAATTGCTCAAAGATGCCATGAATTGGATATGCAGGTTGCAGCACTGCAA
GAGGAGAAAAGTAGTTTATTGGCAGAAAATCAGATACTAATGGAACGACTCAATCAATCTGATTCTATAG
AAGATCCCAATAGCCCAGCAGGAAGAAGACATTTGCAACTTCAGACTCAGTTAGAACAGCTGCAAGAAGA
AACATTCAGACTAGAAGCAGCCAAAGACGATTATCGAATCCGCTGTGAGGAGTTAGAAAAGGAAATCTCT
GAGCTTCGGCAGCAGAACGATGAGCTGACCACTTTGGCAGACGAAGCTCAGTCTCTAAAGGATGAAATAG
ACGTTCTCAGACACTCTTCTGATAAAGTATCTAAACTAGAAGGCCAAGTCGAATCTTATAAGAAGAAGCT
GGAAGACCTTGGAGATTTGAGGCGGCAGGTTAAGCTCTTGGAAGAAAAGAATACGATGTACATGCAGAAC
ACTGTCAGTCTGGAGGAAGAGTTAAGAAAAGCTAATGCCGCCCGGGGACAGCTTGAGACATACAAGAGAC
AAGTTGTAGAGCTGCAAAATAGATTATCTGACGAATCAAAGAAAGCAGATAAGTTAGATTTTGAATATAA
GCGGCTAAAAGAAAAAGTTGATGGTCTTCAGAAAGAAAAAGATAGGTTAAGAACGGAAAGAGATTCTCTG
AAGGAAACCATTGAAGAGTTGCGTTGTGTGCAGGCTCAGGAGGGGCAGCTCACCACTCAAGGGTTAATGC
CTCTGGGAAGCCAGGAATCTTCAGACAGCCTTGCAGCAGAGATTGTTACACCCGAAATCAGGGAGAAACT
TATTCGTCTTCAGCATGAGAATAAAATGTTAAAACTCAATCAAGAGGATTCAGACAATGAGAAAATTGCC
TTATTGCAGAGCCTTCTAGATGATGCAAATCTACGCAAGAATGAGCTGGAGACGGAGAACAGGCTTGTGA
ATCAGAGACTTCTAGAAGTACAGTCACAAGTAGAAGAACTGCAAAAATCTTTGCAGGATCAAGGTTCAAA
AGCAGAAGATGCTATTTCAGTTCTTCTAAAAAAGAAGCTTGAAGAACATCTAGAGAAGCTGCATGAAGCC
AACAATGAATTGCAGAAAAAGAGAGCCATTATTGAAGATCTCGAGCCAAGATTTAACAACAGCTCCTTAA
GAATTGAAGAATTGCAAGAGGCTTTACGGAAGAAAGAAGAAGAAATGAAGCAAATGGAAGAACGCTATAA
AAAATACTTAGAGAAGGCCAAGAGTGTTATCCGTACATTAGACCCTAAACAGAATCAAGGAGCAGCACCA
GAAATTCAAGCTCTTAAAAATCAACTGCAGGAACGGGACCGACTCTTCCACTCATTAGAGAAAGAATATG
AGAAAACGAAGAGTCAAAGAGACATGGAGGAGAAATACATTGTTAGTGCCTGGTACAATATGGGAATGAC
TCTACATAAAAAGGCGGCTGAAGACAGACTAGCTAGCACAGGTTCAGGGCAGTCATTTCTGGCTAGGCAA
AGACAAGCAACCAGCACAAGAAGATCCTACCCAGGCCATGTCCAGCCAGCCACAGCAAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210257 protein sequence
Red=Cloning site Green=Tags(s)

MFNVESVERVELCESLLTWIQTFNVDAPCQTAEDLTNGVVMSQVLQKIDPVYFDDNWLNRIKTEVGDNWR
LKISNLKKILKGILDYNHEILGQQINDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIM
MMEESVQHVVMTAIQELMSKESPVSAGHDAYVDLDRQLKKTTEELNEALSEKEEIAQRCHELDMQVAALQ
EEKSSLLAENQILMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAAKDDYRIRCEELEKEIS
ELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQN
TVSLEEELRKANAARGQLETYKRQVVELQNRLSDESKKADKLDFEYKRLKEKVDGLQKEKDRLRTERDSL
KETIEELRCVQAQEGQLTTQGLMPLGSQESSDSLAAEIVTPEIREKLIRLQHENKMLKLNQEDSDNEKIA
LLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDAISVLLKKKLEEHLEKLHEA
NNELQKKRAIIEDLEPRFNNSSLRIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAAP
EIQALKNQLQERDRLFHSLEKEYEKTKSQRDMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQ
RQATSTRRSYPGHVQPATAR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207659
ORF Size 2160 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207659.1, NM_207659.2, NM_207659.3, NP_997542.1
RefSeq Size 12836 bp
RefSeq ORF 2157 bp
Locus ID 320191
UniProt ID Q8BUK6
Cytogenetics 8 A2
MW 83.5 kDa
Summary Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex (By similarity). Serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (PubMed:12950921). Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_207659" in other vectors (4)
SKU Description Size Price
MC221058 Hook3 (untagged) - Mouse hook homolog 3 (Drosophila) (Hook3), (10ug) 10 ug
$724.00
MG210257 Hook3 (tGFP-tagged) - Mouse hook homolog 3 (Drosophila) (Hook3) 10 ug
$860.00
MR210257L3 Lenti ORF clone of Hook3 (Myc-DDK-tagged) - Mouse hook homolog 3 (Drosophila) (Hook3) 10 ug
$960.00
MR210257L4 Lenti ORF clone of Hook3 (mGFP-tagged) - Mouse hook homolog 3 (Drosophila) (Hook3) 10 ug
$960.00

Plasmid Map for MR210257

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210257
Circular map for MR210257

file-alt Citations

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