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Orc3 (NM_015824) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210227
Orc3 (Myc-DDK-tagged) - Mouse origin recognition complex, subunit 3 (Orc3), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $655.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Orc3
Synonyms Orc3l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210227 representing NM_015824
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACACGGGGCCGCGGACTATGGCCACCTCCTCCGTGTCTAAGGGCTGCTTTGTGTTTAAACCAGACT
TCAAAAAAAGAAAGGTCTTTGTGCCAATAGAGGATTATTTTAATAATGAGGAACTTGACTCTGAGGACAG
TAAACTTCGATTTGAAACATATAGCTTGCTATGGCAACGGATGAAATCTGAAACAGAGCAATTACAGGAG
GAATTAAATGAAAATCTGTTTGATAACCTAGTTGACTTTCTGCAGAAGTCCCATCCTGAATTCCAGAAAA
ATTCAAGAGACTGGGGCTCTCAAATGAAATTCAGAGAAATTCCAACAGCTGCTCTTATTCTTGGTGTGAA
TGTCACGGATCATGATGTGATACTCAGAAGTCTAACAGAGACCCTTCAGAATAATGTCACACCATATGTA
GTCTCACTGCAAGCTAAGGATTGTCCCGATGTGAAACATTTTTTACAAAAGTTTACCTCACAGTTGATGG
ACTGCTGTGTAGACAGGCATTCCAAGGAGGTGACAAGTGGTAAGGCCCTGAAGAAGACAAATTATTCAAT
GGATTCCCTTTGTAGTTGGTATTCGGCAGTCACACAGAAAGCAGACCACAAAGTGACAATCAAAAAGAGG
ACAGCATCTGGCCATTGGCGGTCCCCTCCTGTGGTGCTTATCTTAAAGAGCATGGAGAGTTTCACCAGCA
AAGTCCTCCAAGACTTCATAACTATCAGCAGTCAGCATCTGCATGAGTTTCCGCTAATTCTGATTTTTGG
GATCGCCACATCTCCTGTTATTATCCACCGACTGCTTCCTCATTCAGTGTCATCGCTGTTGTGTGTAGAA
CTGTTCCAGTCTCTGTCTTGCGAGCAACACCTGACAGTAGTTCTTGATAAGCTACTTCTTACACCTCAGT
TTCCCTTTAAACTAAGTAAAAAGGCATTACAGGTTCTGACCAACATCTTTTTGTATCATGATTTCTCAAT
TCAAAGCTTCATCAAAGGAATTAAGCTTTCTCTACTAGAACATTTTTATTCCCAGCCCCTAAGTGTTCTG
TGCTGCGATCTCTCAGAAGCCAAGAAAAGAGTAAATGTTTTCTCAGTTAGTCAATGTGAAAACATCCGCC
GCCTTCCTTCATTTAGGAGGTACGTGGAAAACCAGCCTTTGGGAAAGCAAGTGGCACTGCTGACAAATGA
GACCTTCTTAAAGGAGAAAACTCAATCATTGCTGGAAGACCTGCATGTTTACCATATCAATTACTTCCTT
GTCTTGAGATGTCTTCATAATTTTACCTCTTCTCTTCCCAAGTACCCACTGGGTCGACAGATTAGGGAGC
TGTATTGCACATGTTTAGAAAAGAAGATATGGGATTCGGAGGAGTACAAGTCAGCCTTGCAGCTGCTGAG
AATGCTGGCAAAGGATGAACTGGTGAGCATACTTCAGCGCTGTATTGAGGTTTTAGATTCCTCTACTGAA
AAGCAGCTTGGCAACACAACACAGAAAATAAAGGATTTCCTGACCCAGTTTCAGAACCTCGATGCAGATA
GCAAAGAGGAAGAGGATGCCTGTGGATCACAGCCAAAGGGGCTGCAGAAGACAGACCTGTATCACCTTCA
GAAGTCCTTACTAGAAATGAAAGAGCTAAGGAGAACTAAGAAGCCAACCAAGTTTGAAATGCTCAGAGAA
AATGTTATGAACTTCATCGACAACTTAGTGAGAGACTACCTTCTGCCTCCTGAGAGCCAGCCTCTGCATG
AGGTAGTGTACTTCAGTGCTGCCAACACACTCCGTGAGCACTTAAATGCTGCTCCAAGAATCGCTCTTCA
CACTGCACTCAACAACCCATACTATTACCTGAAGAATGAAGAACTGGAAGGATGTATTCCAAATACTGCC
CCTGACATCTGCATAGCATACAAACTGCACCTGGAGTGTAGCCTGCTGATCAACCTCGTCGACTGGGCAG
AGGCTTTTGCAACAGTTGTGACAGCTGCTGAGAAAATGGATGCAAATTCTACAGTCTCAGAAGAAATGAG
TGAAGTTATTCATGCTCGATTTATTCGAGCTGTTTCTGAATTAGAACTTTTAGGCTTTATAAAACCTACG
AAACAGAAGACTGACCATGTTGCAAGGCTTACATGGGGAGGCTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210227 representing NM_015824
Red=Cloning site Green=Tags(s)

MHTGPRTMATSSVSKGCFVFKPDFKKRKVFVPIEDYFNNEELDSEDSKLRFETYSLLWQRMKSETEQLQE
ELNENLFDNLVDFLQKSHPEFQKNSRDWGSQMKFREIPTAALILGVNVTDHDVILRSLTETLQNNVTPYV
VSLQAKDCPDVKHFLQKFTSQLMDCCVDRHSKEVTSGKALKKTNYSMDSLCSWYSAVTQKADHKVTIKKR
TASGHWRSPPVVLILKSMESFTSKVLQDFITISSQHLHEFPLILIFGIATSPVIIHRLLPHSVSSLLCVE
LFQSLSCEQHLTVVLDKLLLTPQFPFKLSKKALQVLTNIFLYHDFSIQSFIKGIKLSLLEHFYSQPLSVL
CCDLSEAKKRVNVFSVSQCENIRRLPSFRRYVENQPLGKQVALLTNETFLKEKTQSLLEDLHVYHINYFL
VLRCLHNFTSSLPKYPLGRQIRELYCTCLEKKIWDSEEYKSALQLLRMLAKDELVSILQRCIEVLDSSTE
KQLGNTTQKIKDFLTQFQNLDADSKEEEDACGSQPKGLQKTDLYHLQKSLLEMKELRRTKKPTKFEMLRE
NVMNFIDNLVRDYLLPPESQPLHEVVYFSAANTLREHLNAAPRIALHTALNNPYYYLKNEELEGCIPNTA
PDICIAYKLHLECSLLINLVDWAEAFATVVTAAEKMDANSTVSEEMSEVIHARFIRAVSELELLGFIKPT
KQKTDHVARLTWGGC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015824
ORF Size 2145 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015824.4, NP_056639.3
RefSeq Size 6313 bp
RefSeq ORF 2148 bp
Locus ID 50793
UniProt ID Q9JK30
Cytogenetics 4 A5
MW 82.3 kDa
Summary Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity).UniProtKB/Swiss-Prot Function
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"NM_015824" in other vectors (4)
SKU Description Size Price
MC205022 Orc3 (untagged) - Mouse origin recognition complex, subunit 3 (Orc3), transcript variant 1, (10ug) 10 ug
$655.00
MG210227 Orc3 (tGFP-tagged) - Mouse origin recognition complex, subunit 3-like (S 10 ug
$855.00
MR210227L3 Lenti ORF clone of Orc3 (Myc-DDK-tagged) - Mouse origin recognition complex, subunit 3 (Orc3), transcript variant 1 10 ug
$955.00
MR210227L4 Lenti ORF clone of Orc3 (mGFP-tagged) - Mouse origin recognition complex, subunit 3 (Orc3), transcript variant 1 10 ug
$955.00

Plasmid Map for MR210227

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210227
Circular map for MR210227

file-alt Citations

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