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C2cd2l (NM_027909) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210165
C2cd2l (Myc-DDK-tagged) - Mouse C2 calcium-dependent domain containing 2-like (C2cd2l)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $647.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol C2cd2l
Synonyms 1300006O23Rik; Tmem24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210165 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCGGACTGGGGGCAGCGGGATGTGGGCTGGGCGGCCCTGCTGGTTCTCTTCGCCGCCTCGCTGA
TCACGGTATTGGGCTGGATGCTGCAGTATGCCCGGGGTTTGTGGCTGTCGCGAGCCGATGGGGGCCGAGA
CTCCCGACCTGCCTCAGCTGCTGAGCCCGGGGGTTCACTGCGCGAGCTGGGTGTGTGGCGTTCGCTGCTG
CGTTTGCGGGCGACCCGGACCAGCACCCCCGAGGAAGCCGGCGTACGGGGCCTCCTGGCTTCGCTCTTTG
CCTTCAAGTCTTTCCGGGAGAACTGGCAACGGGCTTGGGTGCGAGCCTTGAATGAGCAGGCCTGCAGGGA
CGGGAGCTCCATCCAAATCGCCTTTGAAGAGATACCCCAACTCCCACCAAGAGCCAGCATCAGTCATGTG
ACCTGCGTTGACCAATCAGAGCGCACCATGGTGCTGCATTGCCAGCTCTCTGCTGAGGAGGTGCGCTTCC
CCATCTCTGTGACCCAGCAGTCCCCCGCTGCCGTCTCCATGGAGACCTACCACGTCACTCTGACACTGCC
ACCAACACAGTTGGAAGTCAGCCTGGAGGAAATCCCTGATGAGGGGCTCCTGGTGTCCTGGGCCTTCACT
GACCGCCCAGAACTCAGCCTAAAGGTGCTTCCCAAGTTGCAGACTAGGGAGAGAGATGAGGAACAACCAG
AGCTCTCAACAGTTGAGGAACTGATCAAGGACGCTATAGTCAGCACTCAGCCCGCCATGATGGTCAACCT
CAGGGCCTGCTCTGCCCCAGGAGGCCTGGTACCCAGTGAGAAGCCACCCACGATGTCCCAGGCCCAGCCA
TCCATCCCCAGACCTACCCGATTATTCTTACGGCAGCTTCGAGCATCTCACCTGGGAAGTGAGCTAGGAG
GTACTGAGGAACTGTGCTGTGCCGCTGAGCTTGACAACCCCATGCAACAAAAGTGGACCAAACCCATGAG
GGCGGGCCCCGAGGTGGAATGGACCGAGGACCTAGCTCTGGATCTGGGTCCCCAGAGCCGGGAGCTGACC
CTCAAAGTGCTCCGGAGCAGCAGCTGTGGAGATGCTGAACTCCTTGGCCAAGCCACACTGCCTGTGGGCT
CACCCTCTAGACCGATGTCACGAAGACAGGTGTGCCCACTGACTCCAGGGCCCGGGAAATCCCTGAGCCC
GGCAGCCACCGTGACAGCGGAGCTACATTATGAGCAGGGTTCCCCTCGGAATCTGGGCACGCCCACCTCC
TCCACCCCTCGCCCCAGCATCACACCCACCAAGAAGATTGAGTTGGACCGGACCATCATGCCCGACGGCA
CAGTCGTCACCACTGTCACTACCGTCCAGTCCCGCCCCCGTGTAGATGGCAAACTAGACTCCCCCTCCCG
CTCCCCGTCCAAGGTGGAGGTGACTGAGAAGATGACAACCGTGCTGAGTGAGAGCAGCGGCCCCAGCAAT
GCCTCCCACAGCAGCAGCCGGGAGAGCCACCTTTCCAATGGCTTGGATCCAGTAGCAGAGACAGCCATTC
GCCAGCTGACTGAGCCCAGTGGGCGGGCAGCCAAGAAGACACCCACCAAGAGGAGCACGCTCATCATCTC
TGGTGTTTCCAAGGTGCCCATCGCCCAGGACGAGTTGGTTCTCTCCTTGGGTTACGCGGCATCTCTGGAA
GCCTCGATGCAAGATGATGCAGGAACCAGTGGTGGTCCTTCGTCACCTCCCTCAGACCCCTCAGCCACAT
CCCCAGGACCTGTTGATGCCCTCTCCAGTCCCACAAGTGTCCAGGAGGCAGATGAGACAACACGTTCAGA
CATCTCTGAGAGGCCGTCTGTGGATGATGTTGAGTCAGAAACAGGGTCCACTGGTGCCCTGGAGACCAGA
AGCCTCAAGGATCACAAAGTGAGTTTCCTGCGCAGTGGCACAAAGCTCATCTTCCGCCGGAGGCCCCGAC
AGAAGGAAGCTGGTCTGAGCCAGTCACACGATGACCTGTCCAACACGACGGCCACACCTAGCGTCCGGAA
GAAGGCTGGCAGCTTTTCCCGTCGCCTTATCAAGCGTTTTTCCTTCAAATCCAAACCCAAGGCCAATGGC
AACCCTAGCCCCCAGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210165 protein sequence
Red=Cloning site Green=Tags(s)

MDPDWGQRDVGWAALLVLFAASLITVLGWMLQYARGLWLSRADGGRDSRPASAAEPGGSLRELGVWRSLL
RLRATRTSTPEEAGVRGLLASLFAFKSFRENWQRAWVRALNEQACRDGSSIQIAFEEIPQLPPRASISHV
TCVDQSERTMVLHCQLSAEEVRFPISVTQQSPAAVSMETYHVTLTLPPTQLEVSLEEIPDEGLLVSWAFT
DRPELSLKVLPKLQTRERDEEQPELSTVEELIKDAIVSTQPAMMVNLRACSAPGGLVPSEKPPTMSQAQP
SIPRPTRLFLRQLRASHLGSELGGTEELCCAAELDNPMQQKWTKPMRAGPEVEWTEDLALDLGPQSRELT
LKVLRSSSCGDAELLGQATLPVGSPSRPMSRRQVCPLTPGPGKSLSPAATVTAELHYEQGSPRNLGTPTS
STPRPSITPTKKIELDRTIMPDGTVVTTVTTVQSRPRVDGKLDSPSRSPSKVEVTEKMTTVLSESSGPSN
ASHSSSRESHLSNGLDPVAETAIRQLTEPSGRAAKKTPTKRSTLIISGVSKVPIAQDELVLSLGYAASLE
ASMQDDAGTSGGPSSPPSDPSATSPGPVDALSSPTSVQEADETTRSDISERPSVDDVESETGSTGALETR
SLKDHKVSFLRSGTKLIFRRRPRQKEAGLSQSHDDLSNTTATPSVRKKAGSFSRRLIKRFSFKSKPKANG
NPSPQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027909
ORF Size 2118 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027909.1
RefSeq Size 4334 bp
RefSeq ORF 2121 bp
Locus ID 71764
UniProt ID Q80X80
Cytogenetics 9 A5.2
MW 76.4 kDa
Summary Lipid-binding protein that transports phosphatidylinositol, the precursor of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), from its site of synthesis in the endoplasmic reticulum to the cell membrane (By similarity). It thereby maintains the pool of cell membrane phosphoinositides, which are degraded during phospholipase C (PLC) signaling (By similarity). Plays a key role in the coordination of Ca(2+) and phosphoinositide signaling: localizes to sites of contact between the endoplasmic reticulum and the cell membrane, where it tethers the two bilayers (By similarity). In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane, abolishing phosphatidylinositol transport to the cell membrane (By similarity). Positively regulates insulin secretion in response to glucose (PubMed:24012759). Phosphatidylinositol transfer to the cell membrane allows replenishment of PI(4,5)P2 pools and calcium channel opening, priming a new population of insulin granules (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_027909" in other vectors (4)
SKU Description Size Price
MC202622 C2cd2l (untagged) - Mouse C2 calcium-dependent domain containing 2-like (C2cd2l), (10ug) 10 ug
$647.00
MG210165 C2cd2l (tGFP-tagged) - Mouse transmembrane protein 24 (Tmem24) 10 ug
$847.00
MR210165L3 Lenti ORF clone of C2cd2l (Myc-DDK-tagged) - Mouse C2 calcium-dependent domain containing 2-like (C2cd2l) 10 ug
$947.00
MR210165L4 Lenti ORF clone of C2cd2l (mGFP-tagged) - Mouse C2 calcium-dependent domain containing 2-like (C2cd2l) 10 ug
$947.00

Plasmid Map for MR210165

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210165
Circular map for MR210165

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