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Dhx33 (NM_178367) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210113
Dhx33 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 33 (Dhx33)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $639.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dhx33
Synonyms 3110057P17Rik; 9430096J02Rik; Ddx33
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210113 representing NM_178367
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGAGGAGGCGAGCCTCCCGCCCGCTAAGAGATTCCGGCCGGGCTCCTGCCCTCCCGGGAGACGAG
TAGTGATGCTGTTGACGGCTGGCGGCGGCGGCGGAGCTGGAGGAGGCCGGAGGCAGACGCCGCCGCTGGC
CCAGCCTTCGGCCAGTCCCTACCGCGAGGCCCTGGAGCTGCAGCGCCGGAGTCTGCCCATCTTCCGGGCG
CGGGGGCAGCTATTGGCTCAGCTCCGAAACCTGGACAACGCGGTCCTCATCGGGGAAACTGGCTCTGGGA
AAACAACTCAGATCCCACAGTACCTCTATGAAGGGGGAATTAGCCGCCAGGGCATCATCGCTGTGACCCA
GCCCCGTCGAGTGGCTGCCATCTCTCTTGCCACCAGAGTCTCAGATGAGAAGAGGACAGAACTCGGGAAG
CTGGTTGGCTATACAGTGCGCTTTGAGGATGTCACCTCAGAAGACACCAGGATCAAGTTCCTGACAGATG
GCATGCTTCTGCGTGAAGCAATTTCAGACTCCTTGCTTCGGAAGTACAGCTGTGTCATTTTGGACGAGGC
CCATGAGCGGACTATTCACACAGATGTGCTCTTTGGAGTGGTGAAGACTGCACAGAAGAGGCGAAAGGAG
CTAGGGAAACTGCCTCTCAAAGTGATTGTGATGTCAGCTACCATGGATGTCGACCTGTTTTCTCAGTACT
TCAATAGAGCCCCTGTCCTTTACCTTGAGGGTCGGCAGCATCCAATCCAGATCTTCTATACCAAACAGCC
TCAGCAGGATTACCTGCATGCAGCCCTTGTCTCAGTCTTCCAGATCCACCAGGAAGCTCCTGCTTCTCAA
GACATCCTGGTGTTCCTCACTGGTCAGGAGGAGATCGAAGCAATGAGCAAGACCTGCAGAGACATCGCAA
GGCACCTCCCAGATGGCTGCCCTTCAATGCTCGTCCTTCCTCTGTACGCCTCCCTGCCCTATTCGCAGCA
GCTTCGAGTCTTTCAAGGGGCCCCAAAGGGCTATCGCAAAGTGATCATTTCAACCAACATCGCTGAAACC
TCCATAACCATTACAGGAATAAAATATGTAGTTGACACGGGCATGGTTAAAGCAAAGAAGTATAACCCTG
ACAGTGGTCTTGAGGTACTAGCTGTACAGAGAGTATCAAAGACCCAGGCCTGGCAGCGCACAGGCAGGGC
TGGGCGAGAGGACAGTGGCATCTGCTACCGACTCTACACTGAGGACGAGTTTGAGAAGTTCGAGAAGATG
ACGGTGCCAGAGATCCAGAGGTGTAACCTGGCCAGTGTAATACTTCAGCTCCTAGCAATGAAAGTCCCAA
ATGTGCTCACCTTTGACTTCATGTCCAAACCTTCTCCAGATCACATTGAGGCGGCCATTGCCCAGCTGGA
CCTGTTAGGTGCTCTTGAACATAAGGATGACCAGCTTACCCTGACTCCAATTGGAAGAAAGATGGCAGCA
TTCCCTTTAGAGCCCAGATTTGCCAAAACTATCCTCCTGTCCTCCAAATTCCACTGTACCGAAGAGATTC
TGACCATAGTCTCCCTGCTATCTGTGGACAGTGTCCTTTACAACCCTCCTGCCCGGAGAGATGAGGTGCA
GAGTGTCCGGAAGAAATTCATATCCAGTGAGGGGGATCACATCACCCTGCTCAATATCTATCGGACCTTC
AAAAACATCGGTGGGAATAAGGACTGGTGCAAAGAGAATTTTGTCAACAGCAAGAATATGCTGCTAGTAG
CTGAAGTCAGAGCACAGCTGAGAGAAATCTGCTTAAAGATGTCAATGCCAATCATGTCATCTCGAGGGGA
CATGGAGAGTGTCCGCCGTTGCATGGCTCACAGCCTCTTTATGAATACTGCTGAACTCCAGACAGATGGC
ACCTATGCCACCACGGACACACATCAGCCAGTGGCCATCCACCCATCATCTGTCCTCTTCCACTGCAAGC
CTGCCTGCGTGGTCTACACTTCACTGCTCTACACCAACAAATGCTACATGCGTGACCTCTGTGTGGTGGA
TGCCGAGTGGCTCTACGAGGCTGCCCCTGACTACTTCAGGAGGAAGCTGAGAACGGCCAGAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210113 representing NM_178367
Red=Cloning site Green=Tags(s)

MPEEASLPPAKRFRPGSCPPGRRVVMLLTAGGGGGAGGGRRQTPPLAQPSASPYREALELQRRSLPIFRA
RGQLLAQLRNLDNAVLIGETGSGKTTQIPQYLYEGGISRQGIIAVTQPRRVAAISLATRVSDEKRTELGK
LVGYTVRFEDVTSEDTRIKFLTDGMLLREAISDSLLRKYSCVILDEAHERTIHTDVLFGVVKTAQKRRKE
LGKLPLKVIVMSATMDVDLFSQYFNRAPVLYLEGRQHPIQIFYTKQPQQDYLHAALVSVFQIHQEAPASQ
DILVFLTGQEEIEAMSKTCRDIARHLPDGCPSMLVLPLYASLPYSQQLRVFQGAPKGYRKVIISTNIAET
SITITGIKYVVDTGMVKAKKYNPDSGLEVLAVQRVSKTQAWQRTGRAGREDSGICYRLYTEDEFEKFEKM
TVPEIQRCNLASVILQLLAMKVPNVLTFDFMSKPSPDHIEAAIAQLDLLGALEHKDDQLTLTPIGRKMAA
FPLEPRFAKTILLSSKFHCTEEILTIVSLLSVDSVLYNPPARRDEVQSVRKKFISSEGDHITLLNIYRTF
KNIGGNKDWCKENFVNSKNMLLVAEVRAQLREICLKMSMPIMSSRGDMESVRRCMAHSLFMNTAELQTDG
TYATTDTHQPVAIHPSSVLFHCKPACVVYTSLLYTNKCYMRDLCVVDAEWLYEAAPDYFRRKLRTARN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178367
ORF Size 2094 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178367.4, NP_848144.3
RefSeq Size 5179 bp
RefSeq ORF 2097 bp
Locus ID 216877
UniProt ID Q80VY9
Cytogenetics 11 B4
MW 78.3 kDa
Summary Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing (By similarity) (PubMed:21930779). Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment (PubMed:21930779). In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation (PubMed:26100019). Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells (By similarity). Required for NLRP3 activation induced by viral dsRNA and bacterial RNA (By similarity). In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways (PubMed:24037184).UniProtKB/Swiss-Prot Function
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"NM_178367" in other vectors (3)
SKU Description Size Price
MG210113 Dhx33 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 33 (Dhx33) 10 ug
$839.00
MR210113L3 Lenti ORF clone of Dhx33 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 33 (Dhx33) 10 ug
$939.00
MR210113L4 Lenti ORF clone of Dhx33 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 33 (Dhx33) 10 ug
$939.00

Plasmid Map for MR210113

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210113
Circular map for MR210113

file-alt Citations

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