OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Fbln1 (BC007140) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR210029
Fbln1 (Myc-DDK-tagged) - Mouse fibulin 1 (cDNA clone MGC:6128 IMAGE:3495754)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $627.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Fbln1 or BC007140"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbln1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210029 representing BC007140
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGCCCCGTGCCGTCGCGCCTCGTCCCGCTGCCGCTGCTGCTGCTCAGCAGCCTCTCGCTGCTGG
CAGCCCGAGCGAATGCAGACATCTCCATGGAGGCTTGCTGCACGGATGGAAATCAGATGGCTAACCAGCA
CAGGGACTGCTCGCTGCCGTACACCTCAGAATCCAAGGAGTGCAGGATGGTCCAGGAGCAATGTTGTCAC
AACCAACTGGAAGAGCTGCACTGTGCCACGGGCATCAACCTGGCCAGCGAGCCAGAAGGCTGCGCCTCGC
TCCACAGCTACAACAGTAGCCTTGAGACCATCTTCATAAAGAGGTGCTGCCACTGTTGCATGCTGGGAAA
GGCATCCCTGGCCCGAGACCAGACCTGTGAACCCATTGTCATGATAAGCTACCAGTGTGGGCTGGTGTTC
CGTGCCTGCTGTGTGAAGGCCCGGGAGAATTCAGACTTTGTCCAAGGCAACGGTGCAGACCTTCAGGACC
CAGCTAAGATTCCTGACGAGGAGGACCAAGAAGACCCGTACCTGAATGACCGCTGTCGAGGTGGCGGGCC
CTGTAAGCAGCAGTGCCGTGACACTGGGGACGAGGTGATCTGCTCTTGCTTTGTGGGCTACCAGCTGCAG
TCGGATGGTGTCTCCTGCGAAGATATTAATGAATGCATCACAGGCAGCCATAACTGCCGGCTGGGAGAAT
CCTGCATCAATACAGTGGGCTCTTTCCGCTGCCAGCGGGACAGCAGCTGTGGGACTGGCTATGAGCTCAC
AGAGGATAATAACTGCAAAGATATTGACGAATGTGAGACTGGTATTCATAACTGCCCCCCCGATTTTATC
TGTCAGAATACTCTGGGATCCTTCCGTTGCAGACCGAAGCTGCAGTGCAAGAGCGGCTTCATACAGGATG
CTCTAGGCAACTGCATTGATATCAATGAGTGTTTAAGTATCAGTGCTCCATGCCCTGTGGGGCAGACATG
CATCAATACAGAGGGCTCCTACACATGCCAGAAGAATGTGCCCAACTGTGGCCGTGGTTATCATCTCAAT
GAAGAGGGGACCCGCTGTGTTGATGTGGATGAGTGCTCCCCACCAGCGGAGCCCTGTGGGAAGGGACACC
ACTGCCTGAACTCCCCCGGCAGCTTCCGCTGCGAGTGCAAGGCTGGCTTCTATTTTGATGGCATCAGCAG
GACCTGCGTGGATATCAACGAGTGCCAGCGCTATCCCGGGCGCCTGTGTGGCCACAAGTGTGAGAACACG
CCAGGCTCCTTCCACTGCAGCTGCTCCGCCGGTTTCCGGCTGTCTGTGGACGGCCGGTCTTGTGAAGATG
TGAACGAGTGTCTCAACAGCCCTTGCAGCCAGGAGTGCGCTAATGTCTATGGCTCCTACCAGTGCTATTG
CCGACGAGGTTACCAGCTCAGTGACGTGGATGGGGTCACCTGCGAAGATATTGATGAGTGTGCCCTGCCC
ACTGGAGGTCACATCTGCTCCTACCGCTGCATCAACATCCCCGGAAGCTTCCAGTGCAGCTGCCCCTCAT
CTGGCTACAGGCTAGCTCCCAATGGCCGCAACTGCCAAGACATTGATGAGTGTGTGACCGGCATCCACAA
CTGTTCCATCAACGAGACTTGCTTCAACATCCAGGGCAGCTTCCGCTGTCTGTCCTTTGAATGCCCCGAG
AACTATCGCCGCTCCGCAGACACCCGCTGTGAGCGCTTGCCTTGCCATGAGAACCAGGAGTGCCCCAGGC
TGCCTCTGAGAATAACCTACTACCACCTCTCTTTTCCCACCAACATCCAAGTGCCAGCGGTGGTTTTCCG
CATGGGCCCTTCCAGTGCTGTCCCTGGGGACAGCATGCAGCTGGCCATCACCGCTGGCAATGAGGAGGGC
TTTTTCACCACTCGAAAGGTGAGCCACCACAGTGGGGTGGTGGCCCTCACCAAACCCATCCCTGAGCCCA
GGGACTTGCTCCTGACCGTCAAGATGGATCTTTATCGCCACGGCACAGTCAGCTCCTTTGTGGCCAAGCT
TTTCATCTTTGTGTCTGCAGAGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210029 representing BC007140
Red=Cloning site Green=Tags(s)

MERPVPSRLVPLPLLLLSSLSLLAARANADISMEACCTDGNQMANQHRDCSLPYTSESKECRMVQEQCCH
NQLEELHCATGINLASEPEGCASLHSYNSSLETIFIKRCCHCCMLGKASLARDQTCEPIVMISYQCGLVF
RACCVKARENSDFVQGNGADLQDPAKIPDEEDQEDPYLNDRCRGGGPCKQQCRDTGDEVICSCFVGYQLQ
SDGVSCEDINECITGSHNCRLGESCINTVGSFRCQRDSSCGTGYELTEDNNCKDIDECETGIHNCPPDFI
CQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPVGQTCINTEGSYTCQKNVPNCGRGYHLN
EEGTRCVDVDECSPPAEPCGKGHHCLNSPGSFRCECKAGFYFDGISRTCVDINECQRYPGRLCGHKCENT
PGSFHCSCSAGFRLSVDGRSCEDVNECLNSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALP
TGGHICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGSFRCLSFECPE
NYRRSADTRCERLPCHENQECPRLPLRITYYHLSFPTNIQVPAVVFRMGPSSAVPGDSMQLAITAGNEEG
FFTTRKVSHHSGVVALTKPIPEPRDLLLTVKMDLYRHGTVSSFVAKLFIFVSAEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC007140
ORF Size 2055 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC007140.1
RefSeq Size 2222 bp
RefSeq ORF 2057 bp
Locus ID 14114
Cytogenetics 15 E2
MW 81.4 kDa
Summary Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"BC007140" in other vectors (4)
SKU Description Size Price
MC200624 Fbln1 (untagged) - Mouse fibulin 1 (cDNA clone MGC:6128 IMAGE:3495754), (10ug) 10 ug
$628.00
MG210029 Fbln1 (tGFP-tagged) - Mouse fibulin 1 (cDNA clone MGC:6128 IMAGE:3495754) 10 ug
$827.00
MR210029L3 Lenti ORF clone of Fbln1 (Myc-DDK-tagged) - Mouse fibulin 1 (cDNA clone MGC:6128 IMAGE:3495754) 10 ug
$927.00
MR210029L4 Lenti ORF clone of Fbln1 (mGFP-tagged) - Mouse fibulin 1 (cDNA clone MGC:6128 IMAGE:3495754) 10 ug
$927.00

Plasmid Map for MR210029

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210029
Circular map for MR210029

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products