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Syn1 (NM_013680) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209977
Syn1 (Myc-DDK-tagged) - Mouse synapsin I (Syn1), transcript variant a
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ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $646.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Syn1
Synonyms Syn-1; Syn1-S
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209977 representing NM_013680
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTACCTGCGGCGCCGCCTGTCGGACAGCAACTTCATGGCCAATCTGCCGAATGGGTACATGACAG
ACCTGCAGCGCCCGCAACCGCCCCCGCCGCCTCCCTCGGCCGCCAGCCCTGGGGCCACGCCCGGCTCCGC
GACAGCCTCTGCCGAGAGGGCCTCCACAGCTGCTCCAGTGGCTTCTCCAGCAGCCCCTAGTCCTGGGTCC
TCGGGGGGCGGCGGCTTCTTCTCGTCGCTGTCTAACGCGGTCAAGCAAACCACAGCAGCCGCAGCCGCCA
CCTTCAGCGAGCAGGTGGGCGGTGGCTCTGGGGGCGCAGGCCGCGGGGGCGCCGCCGCCAGGGTGCTGCT
GGTCATCGACGAACCGCACACCGACTGGGCAAAATACTTCAAAGGGAAGAAGATCCATGGAGAAATTGAC
ATTAAAGTAGAGCAAGCTGAATTCTCTGATCTCAATCTTGTGGCTCATGCCAATGGTGGATTCTCTGTGG
ACATGGAAGTTCTTCGGAATGGAGTCAAAGTTGTGAGGTCTCTGAAGCCAGACTTTGTGCTGATCCGCCA
GCATGCCTTCAGCATGGCACGTAATGGAGACTACCGAAGTTTGGTCATTGGGCTGCAGTATGCTGGAATC
CCCAGTGTAAACTCTTTGCATTCTGTCTACAACTTCTGTGACAAACCCTGGGTGTTTGCCCAGATGGTTC
GACTACACAAGAAGCTTGGAACAGAGGAATTCCCTCTGATTGATCAGACTTTCTATCCTAATCACAAAGA
GATGCTCAGCAGCACAACATACCCTGTGGTTGTGAAGATGGGCCACGCACATTCTGGGATGGGCAAGGTC
AAGGTAGACAACCAACATGACTTCCAGGATATTGCAAGTGTTGTGGCACTGACTAAGACATATGCCACTG
CTGAGCCCTTCATTGATGCTAAATATGATGTGCGTGTCCAGAAGATTGGGCAGAACTACAAGGCCTACAT
GAGGACATCAGTGTCGGGTAACTGGAAGACCAATACAGGTTCTGCTATGCTTGAGCAGATTGCCATGTCT
GACAGGTACAAGTTGTGGGTAGACACGTGCTCAGAGATTTTTGGGGGACTTGACATCTGCGCAGTGGAAG
CGCTGCATGGCAAGGACGGAAGGGATCACATTATTGAGGTGGTGGGCTCCTCCATGCCACTCATTGGTGA
TCACCAGGATGAAGACAAGCAGCTCATCGTGGAACTTGTGGTCAACAAGATGACTCAGGCTCTGCCTCGG
CAGCCGCAGCGGGATGCTTCCCCTGGCAGGGGCTCCCACAGCCAGTCTTCATCCCCAGGAGCCCTGACCT
TGGGCCGCCAGACCTCCCAGCAGCCTGCAGGTCCTCCTGCTCAACAACGACCCCCACCCCAGGGAGGCCC
TCCACAGCCAGGCCCAGGACCTCAGCGCCAGGGACCCCCGCTGCAGCAGCGCCCACCCCCACAAGGCCAG
CAACATCTTTCTGGCCTTGGACCGCCAGCTGGCAGCCCTCTGCCTCAGCGCCTACCAAGTCCCACCGCAG
CACCTCAGCAGTCTGCCTCTCAGGCCACACCAGTGACCCAGGGTCAAGGCCGCCAGTCGCGGCCAGTGGC
AGGAGGCCCTGGAGCACCTCCAGCAGCGCGCCCACCAGCCTCCCCATCTCCACAGCGTCAGGCGGGGGCC
CCGCAGGCTACCCGTCAGGCATCTATCTCTGGTCCAGCTCCAACGAAGGCCTCAGGAGCCCCACCCGGAG
GGCAGCAGCGCCAGGGCCCTCCCCAAAAACCCCCAGGCCCTGCTGGTCCCACTCGTCAGGCCAGTCAGGC
AGGTCCCGGACCTCGCACTGGGCCTCCCACCACACAGCAGCCCCGGCCCAGCGGCCCAGGTCCTGCTGGA
CGTCCCGCCAAACCACAGCTGGCCCAGAAACCCAGCCAGGATGTGCCACCACCCATCACCGCCGCTGCCG
GGGGACCCCCGCACCCCCAGCTCAACAAATCCCAGTCTCTGACCAATGCCTTCAACCTTCCAGAGCCAGC
CCCTCCCAGGCCCAGCCTTAGCCAGGACGAGGTGAAAGCTGAGACCATCCGCAGCCTGAGGAAGTCTTTC
GCCAGCCTCTTCTCCGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209977 representing NM_013680
Red=Cloning site Green=Tags(s)

MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPSAASPGATPGSATASAERASTAAPVASPAAPSPGS
SGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAAARVLLVIDEPHTDWAKYFKGKKIHGEID
IKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGI
PSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKV
KVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMS
DRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMTQALPR
QPQRDASPGRGSHSQSSSPGALTLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQ
QHLSGLGPPAGSPLPQRLPSPTAAPQQSASQATPVTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGA
PQATRQASISGPAPTKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPGPRTGPPTTQQPRPSGPGPAG
RPAKPQLAQKPSQDVPPPITAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSF
ASLFSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013680
ORF Size 2118 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013680.4
RefSeq Size 3143 bp
RefSeq ORF 2121 bp
Locus ID 20964
UniProt ID O88935
Cytogenetics X 16.37 cM
MW 74.5 kDa
Summary Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.UniProtKB/Swiss-Prot Function
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"NM_013680" in other vectors (4)
SKU Description Size Price
MC201687 Syn1 (untagged) - Mouse synapsin I (Syn1), transcript variant a, (10ug) 10 ug
$622.00
MG209977 Syn1 (tGFP-tagged) - Mouse synapsin I (Syn1) 10 ug
$846.00
MR209977L3 Lenti ORF clone of Syn1 (Myc-DDK-tagged) - Mouse synapsin I (Syn1), transcript variant a 10 ug
$946.00
MR209977L4 Lenti ORF clone of Syn1 (mGFP-tagged) - Mouse synapsin I (Syn1), transcript variant a 10 ug
$946.00

Plasmid Map for MR209977

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file-download TXT (FASTA format)

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Cloning scheme for MR209977
Circular map for MR209977

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