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Clpb (NM_009191) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209968
Clpb (Myc-DDK-tagged) - Mouse ClpB caseinolytic peptidase B homolog (E. coli) (Clpb)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $621.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Clpb
Synonyms AL118244; Skd3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209968 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTTGTCCGCGGTGTTGAGGAGAACCACGCCAGCGCCTCGGCTCTTCCTAGGGTTAATCAAATCTC
CATCTCTCCAAAGCCGGGGAGGTGCTTACGGCCGTGGCGTTGTCACCGGAGACCGCGGGGAGCCGCAGCG
GTTGAGAGCTGCCGCTTGGGTGCGCCCTGGAGCATCTTCTACCTTCGTCCCCGGACGGGGGGCAGCCACC
TGGGGGCGCCGTGGAGAACGCACCGAGATCCCATACCTGACTGCCGCCTCGTCGGAACGCGGTCCTAGCC
CCGAAGAAACACTCCCGGGACAAGACAGCTGGAATGGGGTCCCCAACAAGACGGGTCTGGGCATGTGGGC
CTTGGCCATGGCGCTGGTGGTTCAGTGCTACAGCAAGAATCCATCCAACAAGGATGCAGCTCTAATGGAA
GCTGCCCGGGCCAATAACGTGCAGGAAGTCAGAAGGCTGTTATCAGAAGGTGCAGATGTCAATGCAAGAC
ACAAACTTGGCTGGACAGCACTGATGGTGGCATCCATCAGCCACAATGAGAGTGTGGTGCAGGTCCTGCT
TGCTGCTGGAGCTGACCCAAACCTTGGGGATGAGTTCAGCAGTGTCTACAAGACTGCCAATGAACAGGGA
GTCCATTCTCTGGAAGTCCTAGTCACTCGAGAGGATGACTTCAACAACCGGCTAAATCACCGTGCTAGCT
TCAAAGGCTGCACAGCCCTGCACTATGCTGTCCTGGCCGATGACTACAGCATCGTCAAGGAGCTGCTGGA
CAGAGGAGCCAACCCCCTTCAGAGGAATGAGATGGGACACACACCCCTGGATTATGCCCGGGAAGGGGAG
GTTATGAAGCTTCTGAAAACTTCTGAGACCAAGTACATGGAGAAGCAGAGGAAGCGGGAGGCAGAGGAGC
GGCGCCGCTTCCCACTGGAGCAGCGACTGAAGGAGCACATCATTGGCCAGGAGAGTGCCATCGCCACCGT
CGGTGCTGCGATCCGGAGGAAGGAGAACGGCTGGTACGATGAGGAGCACCCTCTAGTCTTCCTCTTCTTG
GGATCGTCTGGAATAGGGAAAACCGAGCTGGCCAAGCAGACAGCAAAGTATATGCACAAAGATGCCAAAA
AGGGCTTCATCCGGCTCGACATGTCTGAGTTCCAGGAGCGACATGAGGTAGCCAAGTTTATCGGTTCTCC
ACCAGGCTACATCGGCCATGAAGAGGGTGGCCAGCTGACCAAGAAACTGAAACAGTGCCCAAATGCAGTA
GTCCTCTTTGATGAGGTGGACAAGGCCCATCCAGATGTGCTCACCATCATGCTGCAGCTGTTTGATGAGG
GCCGCCTGACAGATGGCAAAGGGAAGACCATTGACTGCAAGGATGCCATCTTCATCATGACTTCCAATGT
AGCCAGTGATGAGATCGCCCAGCATGCATTGCAGCTCAGACAGGAAGCTTTGGAGATGAGCCGAAATCGA
ATCGCTGAAAACCTTGGGGATGTCCAGATGAGTGACAAGATCACCATCTCCAAGAACTTTAAAGAGAATG
TGATCCGACCCATCTTGAAAGCTCACTTCCGGAGAGACGAGTTTCTGGGACGGATCAATGAGATCGTCTA
CTTCCTCCCTTTCTGCCACTCAGAGCTCATCCAGCTGGTCAACAAGGAACTGAACTTCTGGGCCAAGAGA
GCCAAGCAGAGGCACAACATCACACTACTGTGGGACCGCGAGGTGGCAGATGTGTTGGTTGATGGCTACA
ACGTCCACTATGGTGCCCGCTCCATTAAGCATGAGGTAGAGCGGCGTGTGGTAAACCAACTGGCGGCAGC
CTATGAGCAGGACCTGCTACCAGGGGGTTGCACCCTGCGTATCACTGTGGAGGATTCGGACAAGCACCTC
CTCAAGAGCCCTGAGCTGCCCTCACCCCAGGCTGAGAAGCGTCCACCTACATTGCGACTGGAGATCATTG
ATAAGGACAGCAAGACCCGCAAACTGGACATCCAGGCACCACTCCACCCTGAGAAGGTGTGCTACACCAT
C

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209968 protein sequence
Red=Cloning site Green=Tags(s)

MMLSAVLRRTTPAPRLFLGLIKSPSLQSRGGAYGRGVVTGDRGEPQRLRAAAWVRPGASSTFVPGRGAAT
WGRRGERTEIPYLTAASSERGPSPEETLPGQDSWNGVPNKTGLGMWALAMALVVQCYSKNPSNKDAALME
AARANNVQEVRRLLSEGADVNARHKLGWTALMVASISHNESVVQVLLAAGADPNLGDEFSSVYKTANEQG
VHSLEVLVTREDDFNNRLNHRASFKGCTALHYAVLADDYSIVKELLDRGANPLQRNEMGHTPLDYAREGE
VMKLLKTSETKYMEKQRKREAEERRRFPLEQRLKEHIIGQESAIATVGAAIRRKENGWYDEEHPLVFLFL
GSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEVAKFIGSPPGYIGHEEGGQLTKKLKQCPNAV
VLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTIDCKDAIFIMTSNVASDEIAQHALQLRQEALEMSRNR
IAENLGDVQMSDKITISKNFKENVIRPILKAHFRRDEFLGRINEIVYFLPFCHSELIQLVNKELNFWAKR
AKQRHNITLLWDREVADVLVDGYNVHYGARSIKHEVERRVVNQLAAAYEQDLLPGGCTLRITVEDSDKHL
LKSPELPSPQAEKRPPTLRLEIIDKDSKTRKLDIQAPLHPEKVCYTI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009191
ORF Size 2031 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009191.4
RefSeq Size 4492 bp
RefSeq ORF 2034 bp
Locus ID 20480
UniProt ID Q60649
Cytogenetics 7 E2
MW 76 kDa
Summary May function as a regulatory ATPase and be related to secretion/protein trafficking process.UniProtKB/Swiss-Prot Function
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Documents
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"NM_009191" in other vectors (4)
SKU Description Size Price
MC220326 Clpb (untagged) - Mouse ClpB caseinolytic peptidase B homolog (E. coli) (Clpb), (10ug) 10 ug
$683.00
MG209968 Clpb (tGFP-tagged) - Mouse ClpB caseinolytic peptidase B homolog (E. coli) (Clpb) 10 ug
$821.00
MR209968L3 Lenti ORF clone of Clpb (Myc-DDK-tagged) - Mouse ClpB caseinolytic peptidase B homolog (E. coli) (Clpb) 10 ug
$921.00
MR209968L4 Lenti ORF clone of Clpb (mGFP-tagged) - Mouse ClpB caseinolytic peptidase B homolog (E. coli) (Clpb) 10 ug
$921.00

Plasmid Map for MR209968

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR209968
Circular map for MR209968

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