OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Wwc1 (BC037006) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR209954
Wwc1 (Myc-DDK-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $618.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Wwc1 or BC037006"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wwc1
Synonyms MGC47054, mKIAA0869, RP23-196B5.5, Kibra
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209954 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTCCCTGTCTTCAGGCAGTAGCCCTGGGTCACTCACCTCCAGCCGGGGCTCCCTGGCCGCCTCTA
GCCTGGACTCCTCCACCTCAGCCAGCTTCACTGACCTCTATTATGACCCGTTCGAGCAGTTGGACTCGGA
GCTCCAGAGCAAGGTGGAGCTGCTGTTCCTGGAGGGTGCCACGGGCTTCCGGCCCTCAGGCTGCATCACC
ACCATCCACGAGGATGAGGTGGCCAAGACCCAGAAGGCAGAGGGAGGCAGCCGCCTGCAGGCCCTGCGCT
CCCTATCTGGCACTCCAAGGTCCATGACCTCCCTGTCTCCACGCTCATCTCTCTCCTCCCCATCCCCTCC
CTGTTCCCCTCTCATCACTGATCCCCTCCTGACTGGAGATGCCTTCCTGGCCCCGCTGGAGTTTGAAGAC
ACAGAGCTGAGTACCACTCTCTGTGAGCTGAACCTCGGCGGTAGCGGCACCCAGGAAAGATACCGGCTGG
AGGAACCAGGACCTGAGGGCAAGCCACTGGGCCAAGCTGCAAGTGTGGCCCCAGGATGTGGCCTCAAAGT
AGCCTGTGTGTCCGCTGCAGTGTCCGACGAGTCAGTAGCCGGGGACAGTGGCGTGTATGAGGCTTCAGCA
CAGAGACCAGGTACTTCTGAAGCTGCCGCATTCGACAGTGATGAATCGGAAGCTGTGGGTGCCACTCGGG
TTCAGATTGCCCTGAAATATGATGAGAAGAATAAGCAATTTGCAATATTAATCATTCAGCTGAGTCACCT
CTCTGCTCTGTCACTACAACAAGACCAGAAAGTGAACATCCGTGTAGCCATCCTGCCTTGCTCTGAAAGC
AGCACCTGCTTGTTCAGAACCCGGCCTCTGGACTCGGCAAATACCCTCGTGTTCAATGAGGCATTCTGGG
TGTCCATCTCCTACCCAGCCCTTCACCAAAAGACCTTACGAGTCGATGTCTGCACCACTGACAGGAGCCA
TACAGAGGAGTGTCTGGGAGGTGCCCAGATCAGCCTGGCCGAGGTCTGCCGGTCTGGGGAGAGGTCAACG
CGCTGGTACAATCTCCTGAGCTACAAATACTTGAAGAAGCAGTGCAGGGAGCCCCAGCCAACAGAAGCTC
CAGGGCCAGACCATGTGGACGCTGTGTCTGCCTTGCTAGAACAGACAGCCGTGGAGCTAGAGAAGAGGCA
GGAGGGAAGAAGCAGCTCCCAGACGCTGGAGGGCAGCTGGACGTACGAGGAAGAGGCCAGCGAGAATGAG
GCAGTCGCTGAGGAGGAAGAGGAGGGAGAGGAAGATGTCTTCACTGAAAAGGTCTCTCCCGAGGCAGAGG
AGTGCCCAGCATTAAAGGTGGACAGAGAGACCAATACTGACAGCGTGGCCCCATCCCCCACAGTGGTGCG
CCCTAAGGACCGGAGGGTGGGTGCCCCGTCGACAGGGCCATTTCTTCGAGGGAATACCATCATCCGCTCC
AAGACCTTCTCGCCTGGACCCCAGAGCCAGTATGTGTGTCGGCTAAACCGCAGTGACAGTGATAGTTCTA
CGCTGTCCAAGAAGCCACCTTTTGTGCGAAACTCCCTGGAGCGGCGCAGCGTCAGGATGAAGCGGCCTTC
CTCCGTCAAGTCCCTGCGCACAGAGCGTCTGATCCGCACCTCGCTGGACCTAGAACTCGACCTGCAGGCC
ACGAGGACCTGGCACAGCCAGCTGACTCAGGAGATCTCAGTGCTGAAGGAGCTGAAGGAGCATTTGGAGC
AAGCCAAGAACCACGGGGAGAAGGAGCTGCCACAGTGGCTGCGTGAAGACGAGCGCTTCCGCTTGCTACT
GAGGATGCTGGAGAAGAAGGTGGATCGTGGGGAGCACAAGAGTGAGTTGCAAGCCGACAAGATGATGAGA
GCTGCTGCCAAGGATGTGCACAGGCTTCGAGGCCAGAGCTGTAAGGAGCCCCCAGAAGTGCAGTCTTTCA
GGGAGAAAATGGCATTTTTCACCCGGCCTCGGATGAACATCCCAGCTCTCTCTGCAGATGACGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209954 protein sequence
Red=Cloning site Green=Tags(s)

MQSLSSGSSPGSLTSSRGSLAASSLDSSTSASFTDLYYDPFEQLDSELQSKVELLFLEGATGFRPSGCIT
TIHEDEVAKTQKAEGGSRLQALRSLSGTPRSMTSLSPRSSLSSPSPPCSPLITDPLLTGDAFLAPLEFED
TELSTTLCELNLGGSGTQERYRLEEPGPEGKPLGQAASVAPGCGLKVACVSAAVSDESVAGDSGVYEASA
QRPGTSEAAAFDSDESEAVGATRVQIALKYDEKNKQFAILIIQLSHLSALSLQQDQKVNIRVAILPCSES
STCLFRTRPLDSANTLVFNEAFWVSISYPALHQKTLRVDVCTTDRSHTEECLGGAQISLAEVCRSGERST
RWYNLLSYKYLKKQCREPQPTEAPGPDHVDAVSALLEQTAVELEKRQEGRSSSQTLEGSWTYEEEASENE
AVAEEEEEGEEDVFTEKVSPEAEECPALKVDRETNTDSVAPSPTVVRPKDRRVGAPSTGPFLRGNTIIRS
KTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRTERLIRTSLDLELDLQA
TRTWHSQLTQEISVLKELKEHLEQAKNHGEKELPQWLREDERFRLLLRMLEKKVDRGEHKSELQADKMMR
AAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPALSADDV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC037006
ORF Size 2025 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC037006, AAH37006
RefSeq Size 2583 bp
RefSeq ORF 2027 bp
Locus ID 211652
Cytogenetics 11 A4
MW 74.3 kDa
Summary Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ and may be associated with memory performance (By similarity). Regulates collagen-stimulated activation of the ERK/MAPK cascade.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"BC037006" in other vectors (4)
SKU Description Size Price
MC220895 Wwc1 (untagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674), (10ug) 10 ug
$681.00
MG209954 Wwc1 (tGFP-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674) 10 ug
$818.00
MR209954L3 Lenti ORF clone of Wwc1 (Myc-DDK-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674) 10 ug
$918.00
MR209954L4 Lenti ORF clone of Wwc1 (mGFP-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674) 10 ug
$918.00

Plasmid Map for MR209954

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209954
Circular map for MR209954

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products