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Ndc1 (NM_028355) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209939
Tmem48 (Myc-DDK-tagged) - Mouse transmembrane protein 48 (Tmem48)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $617.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndc1
Synonyms 2810475A17Rik; AI450313; sks; Tmem48
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209939 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACGGCTGCGAGCGGGCCCTGCGCCGGCGGGTCGCCGCGGGACATCCTTTGGCGCGTTTTGGGTT
GGAGGATAGTTACCAGCATCGTTTGGTCAGTAGTGCTTCTACCCGTCTGCATCACAGCCTTTATAGTCTT
AAGCAGCATCAATTTATTCCACCCTATACAGTGGCTGTCAGATTCTTGCAATGACTTCTATAGTTCCCAA
GTCATCTTTCACCTCCTGCTGCTGGCCGTGGTCATTATAATAATAAGTATTTTCAATGTGGAATTCTATA
CAGTTGTGCCCTCCATATCCGGCTCCAGACTGGCCCTGATAGCGCGGATCCTGCATCCCCAGCAGCTCAC
GCACTCCTTCATCCACGCCGCGATGGGAATGGCTGTGGCTTGGTGTGCGGCCATCATGACCAAGGGCCAG
TACAGCTCTCTCGTGGTTCCCTGCACTGGAACTGAGAGCTTAGATAGCCCTGCTGCTCAGACCTGCTTGA
ATGAATATCATCTGTTTTTCCTACTGTCCGGAGCATTCATGGGCTATAGCTATAGTCTTCTGTATTTCAT
TAACAACATGAATTATCTCCCGTTTCCTATTATACAGCAGTACAAATTTTTGCGTTTCCGGAGATCTCTC
CTCTTACTAGTTAAACATAGCTGTGTGGAATCCCTGTTCATGGTCAGAAATTTCTGCATTGTGTATTATT
TTTTTGGCCATATTCCCAAAGCATGGATTAGCACTGCTCTGGACCTTCACACAGATGAGCAGGCTCACAG
GCCTCTTGACACGATAGGCGGTCTCCTGAACGTCTCATTACTCTACCACGTCTGGCTGTGTGGCGTCTTC
CTCTTGGTGACTTGGTATAGCTCATGGATACTCTTCAAAATCTATGCCACCGAGGCGCATGTGTTCCCTG
TTCAGCCGCCTTTTGCAGAAGCCTCAGATGAATGCCTTCCTAAAGTCTTAAACAGCAACCCCCCACGCAT
CGTAAAGTATTTGGCCTTACAGGACCTGATGCTGCTTTCTCAATATTCTCCTTCTCGGAGGCAAGAAGTT
TTCAGTCTCAGCCAACCAGGTGGACATCCCCACAATTGGACTGCTATTTCGAGGGAGTGTTTGAATCTTT
TAAATGATATGACTCAGAAACTGGTTCTCTACCAAGAAGCTGCTGCTACAAATGGGAGGATGTATTCATC
ATACTCAGTGGAACCCAAGAAACTGAGCTCTGCAGAAGAAACTGCTTTTCAGACTCCAAAACCTAGTCAG
ACGCCTTCCGTGCCGCCGTTAGTTAAAACTTCACTGTTTTCTCCAAAATTATCTACACCCAATGTTTCAA
GTCCTTTTGGGACCCCGTTTGGCTCCAGCGTAGTGAACCGAATGGCTGGAATTCTTGATGTAAACCCCTT
CTCTGGGTCTCCCCAAAGTCCTCAGCTCATAAGAAGAGGACCGAGGCTGTGGACTCACACTTCTGATCAA
CAAGTGTCTGCAATTTCTAACCCATCTCCTTGCGCCTCTGTTACTGCTGAGGGTAAGACAGTGAGACAGC
CCAGTGTGATCTACTCGTGGATTCAGAATAAACGAGAACAGATTAAGAATTTCTTGTCAAAACGGGTGCT
GATAATGTACTTTTTCAGTAAGCACCCAGAGGCCTCCATTCAGGCTGTTTTTTCAGATGCCCAAATGCAT
ATTTGGGCTTTAGAAGGTCTGTCTCACTTAGTAGCAGCGTCATTTACAGAAGATAGATTTGGAGTTGTCC
AGACCACACTCCCAGCTATCCTTCATACGCTGTTGACTCTGCAGGAGGCTGTCGACAAGTACTTCAAGCT
GCCTCATGCTTCTAGTAAACCACCCCGAGCTTCAGGAAGCCTTGTGGACACGTCCTACAAAACATTGAGA
TTTGCTTTCAGGGCCTCCCTGAAAACCGCTATCTACCGTATAACCACCACGTTTGGTGAACATCTGAACG
CTGTGCAGGCATCTGCAGAACATCAGAAAAGACTTCAGCAATTCTTGGAGTTCAAAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209939 protein sequence
Red=Cloning site Green=Tags(s)

MATAASGPCAGGSPRDILWRVLGWRIVTSIVWSVVLLPVCITAFIVLSSINLFHPIQWLSDSCNDFYSSQ
VIFHLLLLAVVIIIISIFNVEFYTVVPSISGSRLALIARILHPQQLTHSFIHAAMGMAVAWCAAIMTKGQ
YSSLVVPCTGTESLDSPAAQTCLNEYHLFFLLSGAFMGYSYSLLYFINNMNYLPFPIIQQYKFLRFRRSL
LLLVKHSCVESLFMVRNFCIVYYFFGHIPKAWISTALDLHTDEQAHRPLDTIGGLLNVSLLYHVWLCGVF
LLVTWYSSWILFKIYATEAHVFPVQPPFAEASDECLPKVLNSNPPRIVKYLALQDLMLLSQYSPSRRQEV
FSLSQPGGHPHNWTAISRECLNLLNDMTQKLVLYQEAAATNGRMYSSYSVEPKKLSSAEETAFQTPKPSQ
TPSVPPLVKTSLFSPKLSTPNVSSPFGTPFGSSVVNRMAGILDVNPFSGSPQSPQLIRRGPRLWTHTSDQ
QVSAISNPSPCASVTAEGKTVRQPSVIYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQMH
IWALEGLSHLVAASFTEDRFGVVQTTLPAILHTLLTLQEAVDKYFKLPHASSKPPRASGSLVDTSYKTLR
FAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028355
ORF Size 2019 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028355.4
RefSeq Size 2576 bp
RefSeq ORF 2022 bp
Locus ID 72787
UniProt ID Q8VCB1
Cytogenetics 4 C7
MW 75.4 kDa
Summary Component of the nuclear pore complex (NPC), which plays a key role in de novo assembly and insertion of NPC in the nuclear envelope. Required for NPC and nuclear envelope assembly, possibly by forming a link between the nuclear envelope membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane (By similarity).UniProtKB/Swiss-Prot Function
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"NM_028355" in other vectors (4)
SKU Description Size Price
MC202949 Ndc1 (untagged) - Mouse transmembrane protein 48 (Tmem48), (10ug) 10 ug
$617.00
MG209939 Tmem48 (tGFP-tagged) - Mouse transmembrane protein 48 (Tmem48) 10 ug
$817.00
MR209939L3 Lenti ORF clone of Tmem48 (Myc-DDK-tagged) - Mouse transmembrane protein 48 (Tmem48) 10 ug
$917.00
MR209939L4 Lenti ORF clone of Tmem48 (mGFP-tagged) - Mouse transmembrane protein 48 (Tmem48) 10 ug
$917.00

Plasmid Map for MR209939

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209939
Circular map for MR209939

file-alt Citations

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