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Rnf214 (NM_178709) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209899
Rnf214 (Myc-DDK-tagged) - Mouse ring finger protein 214 (Rnf214)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $613.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf214
Synonyms D130054N24Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209899 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCTCTGAGGTAGCTGGCCTTGGGGCCGGTACCCCGAGTCCTTCGGAATCTTCTGCTTTGTGTG
CATCCAAGTCCGATGAATCTCTCCCAGATGGCCTAAGCCCCAAAGACTCTGCCCAGAAACAGAAGAACTT
GTCTCCGCCGAGTGTAAGTAGCCAAATGATAACCAAGGAGAGTAACAGAAATGCCCATTTGGAACATCCA
GAACAGAATCCTGGTTCATCAGTGGGTGATACTTCAGCAGCGCACGAGGAAGTTGTAGGAGAAAACTTGG
TAGCCACAGCCCTTTGTCTTTCTGGCAATGGGTCTCAGTCTGATTTGAAGGACTTGACCAACCCAGCAGG
AGAGGAGGGGGACACGAGCCTCCGGGAGAGCCTCCATCCAGTCACTCGGTCTCTTAAGGCAGGGTGCCAC
TCAAAACAACTTGCCTCCGGGAATTGCTCTGAAGAGAAATGCCCAGCAGCCTCCGTCCTAAAGGAAGGTA
GCAGGGATGCAGGCTTGGACTTGCTACCTGTAGTACCGCCTGCAAATGGGGTTGAAGGAGTGAGAGTGGA
TCAGGATGATGATCAGGATAGCTCTTCCTTGAAGCTTTCTCAGAACATTGCTGTGCAGACTGACTTTAAA
ACAGCAGATTCGGAAGTAAACACAGACCAAGATATTGAAAAGAATCTGGACAAAATGATGACAGAGAGAA
CTCTGTTGAAGGAGCGTTACCAAGAAGTCCTGGACAAGCAGAGGCAGGTGGAGAGTCAGCTCCAGGTGCA
GTTAAAGCAGCTTCAGCAAAGGCGAGAAGAAGAAATGAAGAATCACCAGGAGATACTAAAGGCCATTCAG
GATGTGACCATAAAGCGAGAAGAAACCAAGAAGAAGATAGAGAAAGAAAAGAAGGAGTTCTTGCAGAAGG
AGCAGGACCTGAAAGCTGAAATCGAGAAGCTCTGTGAGAAGGGCAGAAGAGAAGTGTGGGAAATGGAACT
GGATAGACTCAAGAACCAGGATGGTGAAATCAACCGGAATATTATGGAAGAGACAGAGCGGGCCTGGAAA
GCAGAGATACTGTCACTAGAGAGCCGGAAGGAGTTGCTGGTGTTGAAACTGGAAGAAGCAGAAAAAGAAG
CAGAACTTCATCTGACTTACCTCAAGTCAACTCCTCCAACACTAGAGACAGTTCGTTCCAAACAAGAGTG
GGAGACAAGACTGAATGGAGTTCGGATAATGAAAAAGAATGTTCGGGACCAGTTTAATAGTCATATCCAG
CTAGTGAGGAATGGAGCCAAGCTGAGCAGCCTTCCTCAGATCCCTACCCCAACTTTGCCTCCACCACCCT
CAGAGGCAGATTTCATGCTTCAAGTATTTCAGCCCAGTCCCTCTCTGACCCCTCGGATGCCCTTCTCTAT
CGGGCAGGTCACTATGCCTATGGTTATGCCCAGTGCGGATCCTCGCTCTTTGTCTTTTCCGATCTTGAAC
CCTGCCCTTTCCCAGTCCAGCCAACCTTCCCCACCTCTTCCTGGCTCACATGGGAGAAATAGCCCTGGCT
TGGGTTCCCTGGTCAGCCCTCATGGTCCACACATGCCTCCTGCTGCCTCCATCCCGCCTCCCCCAGGCTT
GGGCGGTATTAAGGCTTCTTCTGAAACTCCCCGGCCCCAACCAGTAGACAAACTGGAGAAGATCCTGGAG
AAGCTGCTGACTCGGTTCCCACAGTGCAATAAGGCCCAGATGACCAACATTCTTCAGCAAATCAAGACAG
CACGTACCACCATGGCGGGCCTGACCATGGAGGAACTAATCCAACTGGTAGCCGCACGACTGGCAGAGCA
TGAACGGGTGGCATCAAGCACTCAGGCTCCACCCACTTGTAAACTGTGTCTGATGTGTCAGAAACTTGTC
CAGCCCAGTGAGCTGCACCCGATGGCCTGCACCCACGCACTGCACAAGGAGTGTATCAAATTCTGGGCCC
AGACCAACACAAATGACACTTGTCCCTTTTGCCCAACTCTTAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209899 protein sequence
Red=Cloning site Green=Tags(s)

MAASEVAGLGAGTPSPSESSALCASKSDESLPDGLSPKDSAQKQKNLSPPSVSSQMITKESNRNAHLEHP
EQNPGSSVGDTSAAHEEVVGENLVATALCLSGNGSQSDLKDLTNPAGEEGDTSLRESLHPVTRSLKAGCH
SKQLASGNCSEEKCPAASVLKEGSRDAGLDLLPVVPPANGVEGVRVDQDDDQDSSSLKLSQNIAVQTDFK
TADSEVNTDQDIEKNLDKMMTERTLLKERYQEVLDKQRQVESQLQVQLKQLQQRREEEMKNHQEILKAIQ
DVTIKREETKKKIEKEKKEFLQKEQDLKAEIEKLCEKGRREVWEMELDRLKNQDGEINRNIMEETERAWK
AEILSLESRKELLVLKLEEAEKEAELHLTYLKSTPPTLETVRSKQEWETRLNGVRIMKKNVRDQFNSHIQ
LVRNGAKLSSLPQIPTPTLPPPPSEADFMLQVFQPSPSLTPRMPFSIGQVTMPMVMPSADPRSLSFPILN
PALSQSSQPSPPLPGSHGRNSPGLGSLVSPHGPHMPPAASIPPPPGLGGIKASSETPRPQPVDKLEKILE
KLLTRFPQCNKAQMTNILQQIKTARTTMAGLTMEELIQLVAARLAEHERVASSTQAPPTCKLCLMCQKLV
QPSELHPMACTHALHKECIKFWAQTNTNDTCPFCPTLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178709
ORF Size 2004 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178709.5
RefSeq Size 3782 bp
RefSeq ORF 2007 bp
Locus ID 235315
UniProt ID Q8BFU3
Cytogenetics 9 A5.2
MW 73.6 kDa
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"NM_178709" in other vectors (4)
SKU Description Size Price
MC220226 Rnf214 (untagged) - Mouse ring finger protein 214 (Rnf214), (10ug) 10 ug
$674.00
MG209899 Rnf214 (tGFP-tagged) - Mouse ring finger protein 214 (Rnf214) 10 ug
$813.00
MR209899L3 Lenti ORF clone of Rnf214 (Myc-DDK-tagged) - Mouse ring finger protein 214 (Rnf214) 10 ug
$913.00
MR209899L4 Lenti ORF clone of Rnf214 (mGFP-tagged) - Mouse ring finger protein 214 (Rnf214) 10 ug
$913.00

Plasmid Map for MR209899

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209899
Circular map for MR209899

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