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Sdha (NM_023281) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209872
Sdha (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (Sdha), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $619.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sdha
Synonyms 1500032O14Rik; 2310034D06Rik; 4921513A11; C81073; FP; SDH2; SDHF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209872 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGGTTGGCGCAGTTTCGAGGCTTCTTCGCGGGCGGCGCTTGGCGTTAACTGGGGCGTGGCCAG
GAACACTCCAAAAACAGACCTGCGGCTTTCACTTCTCTGTTGGTGAGAACAAGAAGGCATCAGCTAAAGT
TTCAGATGCAATTTCTACTCAATACCCAGTGGTGGATCATGAATTTGATGCTGTGGTTGTAGGCGCTGGA
GGGGCAGGCTTGCGAGCTGCATTTGGCCTTTCTGAGGCAGGGTTTAATACTGCATGCCTTACAAAGCTCT
TTCCTACCCGATCACATACTGTTGCAGCACAGGGAGGTATCAATGCTGCTCTGGGGAACATGGAAGAGGA
CAACTGGAGATGGCATTTCTATGACACTGTGAAAGGCTCCGACTGGCTGGGGGATCAGGATGCCATCCAT
TACATGACAGAGCAAGCTCCTGCCTCTGTGGTTGAGCTAGAAAATTATGGTATGCCATTTAGCAGAACTG
AAGATGGGAAGATTTATCAGCGTGCATTTGGTGGACAGAGCCTCAAGTTTGGGAAAGGCGGGCAGGCTCA
TCGGTGTTGCTGTGTGGCTGATCGGACAGGCCACTCACTCTTACACACGTTGTATGGAAGATCTCTGCGA
TATGACACCAGTTATTTTGTGGAATATTTTGCACTGGATCTTCTGATGGAAAATGGGGAGTGCCGTGGTG
TCATTGCACTGTGCATAGAAGATGGGTCCATACACCGAATAAGAGCAAAAAACACTGTTATTGCTACTGG
GGGCTACGGGCGAACCTACTTCAGCTGTACATCTGCCCATACCAGCACAGGGGACGGCACAGCCATGGTC
ACTAGGGCTGGTTTGCCTTGCCAGGACTTAGAATTTGTTCAGTTCCACCCCACAGGTATATACGGTGCTG
GCTGCCTCATCACAGAAGGGTGTCGTGGAGAGGGGGGGATTCTCATCAACAGTCAAGGTGAAAGGTTCAT
GGAGAGATACGCACCTGTTGCCAAGGACCTGGCATCAAGAGATGTTGTGTCTCGATCCATGACTCTTGAG
ATCCGTGAAGGAAGAGGCTGTGGCCCTGAGAAAGATCACGTCTACCTGCAGTTGCATCATCTGCCTCCTG
AGCAACTGGCCACACGTCTGCCCGGAATTTCAGAAACGGCCATGATCTTCGCTGGTGTGGATGTCACTAA
GGAGCCCATCCCAGTCCTCCCCACTGTGCATTACAACATGGGTGGGATTCCCACTAACTACAAGGGACAG
GTGCTGAAGCATGTGAATGGCCAGGATCAGATTGTGCCTGGTCTGTATGCCTGTGGGGAGGCTGCCTGCG
CCTCAGTGCATGGTGCCAACCGGCTTGGAGCAAATTCTCTCTTGGACCTTGTAGTCTTTGGCAGAGCCTG
TGCCCTGAGCATTGCAGAATCTTGCAGGCCTGGAGATAAAGTTCCTTCAATTAAGGCAAATGCTGGAGAA
GAATCGGTTATGAATCTTGACAAGTTGAGATTTGCCGATGGAAGTATAAGAACATCAGAACTGCGCCTAA
ACATGCAGAAGTCGATGCAGAACCATGCTGCAGTGTTCCGTGTGGGAAGTGTATTGCAAGAAGGCTGTGA
AAAAATCAGTCAGCTCTATGGAGACCTAAAGCATCTAAAGACATTTGACAGGGGAATGGTTTGGAACACA
GACCTGGTGGAGACCCTGGAGCTGCAGAATCTGATGCTGTGCGCACTGCAGACCATATATGGTGCAGAAG
CTCGGAAGGAGTCCCGGGGAGCCCATGCCAGGGAAGATTACAAAGTGCGGGTCGATGAGTATGATTACTC
CAAGCCCATCCAGGGACAGCAGAAGAAGCCGTTTGGGGAACACTGGAGGAAGCACACGCTCTCATATGTG
GACATCAAGACTGGCAAGGTTACTTTGGAGTACAGACCTGTTATCGACAAGACCTTGAATGAGGCTGACT
GTGCCACCGTACCTCCTGCTATCCGTTCCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209872 protein sequence
Red=Cloning site Green=Tags(s)

MAGVGAVSRLLRGRRLALTGAWPGTLQKQTCGFHFSVGENKKASAKVSDAISTQYPVVDHEFDAVVVGAG
GAGLRAAFGLSEAGFNTACLTKLFPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIH
YMTEQAPASVVELENYGMPFSRTEDGKIYQRAFGGQSLKFGKGGQAHRCCCVADRTGHSLLHTLYGRSLR
YDTSYFVEYFALDLLMENGECRGVIALCIEDGSIHRIRAKNTVIATGGYGRTYFSCTSAHTSTGDGTAMV
TRAGLPCQDLEFVQFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLE
IREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQ
VLKHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIAESCRPGDKVPSIKANAGE
ESVMNLDKLRFADGSIRTSELRLNMQKSMQNHAAVFRVGSVLQEGCEKISQLYGDLKHLKTFDRGMVWNT
DLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRVDEYDYSKPIQGQQKKPFGEHWRKHTLSYV
DIKTGKVTLEYRPVIDKTLNEADCATVPPAIRSY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023281
ORF Size 1992 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023281.1, NP_075770.1
RefSeq Size 2859 bp
RefSeq ORF 1995 bp
Locus ID 66945
UniProt ID Q8K2B3
Cytogenetics 13 C1
MW 72.6 kDa
Summary Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.UniProtKB/Swiss-Prot Function
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"NM_023281" in other vectors (4)
SKU Description Size Price
MC220228 Sdha (untagged) - Mouse succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (Sdha), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$681.00
MG209872 Sdha (tGFP-tagged) - Mouse succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (Sdha) 10 ug
$819.00
MR209872L3 Lenti ORF clone of Sdha (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (Sdha), nuclear gene encoding mitochondrial protein 10 ug
$919.00
MR209872L4 Lenti ORF clone of Sdha (mGFP-tagged) - Mouse succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (Sdha), nuclear gene encoding mitochondrial protein 10 ug
$919.00

Plasmid Map for MR209872

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209872
Circular map for MR209872

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