Hspa5 (NM_001163434) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR209794
Hspa5 (Myc-DDK-tagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 1
$916.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hspa5 |
| Synonyms | AL022860; AU019543; baffled; Bip; D2Wsu17e; D2Wsu141e; Grp78; Hsce70; mBiP; SEZ-7; Sez7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001163434 |
| ORF Size | 1965 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001163434.1, NP_001156906.1 |
| RefSeq Size | 2534 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 14828 |
| UniProt ID | P20029 |
| Cytogenetics | 2 22.94 cM |
| MW | 72.4 kDa |
| Summary | Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:12411443, PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (PubMed:12411443). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001163434" in other vectors (2)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR209794L3 | Lenti ORF clone of Hspa5 (Myc-DDK-tagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 1 | 10 ug |
$1,216.00
|
|
| MR209794L4 | Lenti ORF clone of Hspa5 (mGFP-tagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 1 | 10 ug |
$1,216.00
|
|
Citations
Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity
Sun, Liao, Qu et al
Cell Rep (2020) 30 (6), 1835-1847.e9 DOI: 10.1016/j.celrep.2020.01.043
Sun, Liao, Qu et al
Cell Rep (2020) 30 (6), 1835-1847.e9 DOI: 10.1016/j.celrep.2020.01.043
Cited by 20
Endoplasmic Reticulum Stress Protein GRP78 Modulates Lipid Metabolism to Control Drug Sensitivity and Antitumor Immunity in Breast Cancer
Cook, Soto-Pantoja, Clarke et al
Cancer Res. (2016) 76 (19), 5657-5670 DOI: 10.1158/0008-5472.CAN-15-2616
Cook, Soto-Pantoja, Clarke et al
Cancer Res. (2016) 76 (19), 5657-5670 DOI: 10.1158/0008-5472.CAN-15-2616
Showing 1-2 of 2 papers.
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