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Arhgap24 (BC023344) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209791
Arhgap24 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $609.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgap24
Synonyms MGC38749
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209791 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGCCAATCATGAGAGCTATCTCCTCATGGCGAGCACCCAGAATGACATGGAAGACTGGGTGAAGT
CCATCCGCCGAGTCATATGGGGACCTTTTGGAGGAGGTATTTTTGGACAGAAACTGGAAGACACAGTCCG
ATATGAAAAGAGGTACGGGAACCGGCTGGCACCCATGCTGGTGGAGCAGTGTGTGGACTTCATCCGACAG
CGGGGGCTGAAGGAAGAAGGCCTCTTCCGGCTGCCGGGCCAGGCGAACCTGGTCAAGGAGCTCCAGGATG
CGTTCGACTGTGGAGAGAAGCCCTCCTTCGACAGCAACACCGACGTCCACACTGTGGCATCCCTGCTGAA
ATTGTATCTCCGAGAACTTCCAGAGCCCGTCGTTCCTTATGCAAAGTATGAAGACTTTTTGTCCTGCGCC
ACGCTGCTCAGTAAGGAGGAAGAAGCAGGTGTTAAGGAGCTAATGAAACAAGTGAAGAGTTTGCCTGTGG
TTAACTACAACCTTCTGAAGTACATTTGCAGGTTCCTGGATGAAGTGCAGTCCTATTCAGGAGTTAACAA
GATGAGCGCACAGAACTTGGCAACTGTCTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGATCCCTTG
ACCATCATGGAGGGCACTGTGGTGGTCCAGCAGTTAATGTCAGTGATGATTAGTAAGCATGATCGACTCT
TCCCCAAAGACACAGAACCACAAAGCAAGCCCCAAGACGGCCCGAACAGCAATAATAACGATGGTCATAA
GAAAGCCACCATGGGTCAGTTGCAGAACAAAGAAAACAATAATACCAAGGAGAGCCCCGTCAGAAGGTGC
TCTTGGGACAAGCCTGAATCCCCCCAGAGAAGCAGCGTGGACAATGGATCCCCCACAGCCCTATCAGGCA
GCAAAACCAACAGCCCAAGGAACAGTATCCACAAGCTGGATATTTCCAGGAGCCCCCCTCTCATGGTCAA
AAAGAACCCAGCCTTCAACAAGGGCAGTGGGATTGTCACCAACGGGTCATTCAGCAGCAGCAATGCAGAA
GGTGTCGAGAAGCCTCAAACTACTCCTAATGGGAGTTTACAAGCCAGAAGGACCTCTTCACTGAAGAGTT
CTGGTACCAAAATGGGCACTCACAGTGTACAGAATGGCACTGTCCGCATGGGTATTCTGAACACTGACAC
ACTAGGGAATTCCTTGAACGGCCGGAGCATGAGTTGGCTGCCCAATGGCTATGTGACCCTGAGAGATAAC
AAACAGAAGGAGCCAGCAGGAGAGTCAGGCCAGCACAACAGACTCTCCACCTACGACAATGTCCACCAGC
AGTTCTCCTCGATGAGCCTCGACGACAAGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTCCTGCGA
AATCTCCCTCCCTGAGAACTCCAATTCCTGCCGCTCCTCTACCACCACGTGCCCAGAGCAAGACTTCTAC
GTTGGGAACTTTGAGGACCCTGTTTTAGACGGCCCACCTCAGGATGATCTTTCCCATCCCGGGGACTATG
AGAACAAAAGTGACCGCAGGAGCGTAGGGGGCCGAAGCAGCCGCGCCACGAGCAGCAGCGATAACAGCGA
GACATTCGTGGGCAACACCAGCAGCAACCACAGTGCACTTCACAGTTTAGTTTCCAGCCTGAAGCAGGAG
ATGACCAAACAGAAGATCGAGTATGAGTCCAGGATAAAAAGCTTAGAACAGCGGAATTTGACTTTGGAAA
CGGAAATGCTGAGCCTCCATGACGAACTGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAATCAAGAT
GAGAAACGCTGAGCGCGCAAAGGAGGATGCGGAGAAAAGGAACGACATGCTCCAGAAGGAGATGGAGCAG
TTCTTCTCCACATTTGGAGACCTGACAGTGGAACCCAGGAGAAGCGAGAGAGGAAACACAATCTGGATCC
AG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209791 protein sequence
Red=Cloning site Green=Tags(s)

MTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQ
RGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVVPYAKYEDFLSCA
TLLSKEEEAGVKELMKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSAQNLATVFGPNILRPKVEDPL
TIMEGTVVVQQLMSVMISKHDRLFPKDTEPQSKPQDGPNSNNNDGHKKATMGQLQNKENNNTKESPVRRC
SWDKPESPQRSSVDNGSPTALSGSKTNSPRNSIHKLDISRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAE
GVEKPQTTPNGSLQARRTSSLKSSGTKMGTHSVQNGTVRMGILNTDTLGNSLNGRSMSWLPNGYVTLRDN
KQKEPAGESGQHNRLSTYDNVHQQFSSMSLDDKHSVDSATWSTSSCEISLPENSNSCRSSTTTCPEQDFY
VGNFEDPVLDGPPQDDLSHPGDYENKSDRRSVGGRSSRATSSSDNSETFVGNTSSNHSALHSLVSSLKQE
MTKQKIEYESRIKSLEQRNLTLETEMLSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQ
FFSTFGDLTVEPRRSERGNTIWIQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC023344
ORF Size 1962 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023344, AAH23344
RefSeq Size 2732 bp
RefSeq ORF 1964 bp
Locus ID 231532
Cytogenetics 5 E5
MW 73.3 kDa
Summary Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis (By similarity).UniProtKB/Swiss-Prot Function
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"BC023344" in other vectors (3)
SKU Description Size Price
MG209791 Arhgap24 (tGFP-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885) 10 ug
$809.00
MR209791L3 Lenti ORF clone of Arhgap24 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885) 10 ug
$909.00
MR209791L4 Lenti ORF clone of Arhgap24 (mGFP-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885) 10 ug
$909.00

Plasmid Map for MR209791

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209791
Circular map for MR209791

file-alt Citations

Glucose Transporter 4 (GLUT4) is Not Necessary for Overload-Induced Glucose Uptake or Hypertrophic Growth in Mouse Skeletal Muscle
McMillin, Schmidt, Kahn et al
Diabetes (2017)
AI Summary: OriGene products, including plasmids for mouse GLUT1 (MR207871), GLUT3 (MR2097915), GLUT6 (MR219710), GLUT10 (MR227535), and a HEK293 cell GLUT10 overexpression lysate (LY410718), were used to study the expression and potential roles of alternative glucose transporters in mouse skeletal muscle during overload-induced glucose uptake.
Snippet: Plasmids containing mouse GLUT1 (catalog #MR207871), GLUT3 (catalog #MR2097915), GLUT6 (catalog #MR219710), and GLUT10 (catalog #MR227535) and an HEK293 cell GLUT10 overexpression lysate (catalog #LY410718) were purchased from OriGene;;... … Plasmids containing mouse GLUT1 (cat#MR207871), GLUT3 (cat#MR2097915), GLUT6 (cat#MR219710) and GLUT10 (cat#MR227535), and a HEK293 cell GLUT10 overexpression lysate (cat#LY410718) were purchased from Origene Technologies. ... …
Showing 1-1 of 1 papers.
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