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Rnf112 (NM_009548) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209790
Rnf112 (Myc-DDK-tagged) - Mouse ring finger protein 112 (Rnf112)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $610.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf112
Synonyms bfp; neurolastin; Zfp179; ZNF179
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209790 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAGGCCCGTCCTGTCAGTCACTGCTTTTTGTCATCGGCTTGGCAAACGGGAGAGCAAACGAAGCT
TCATGGGAAACAGCAGCAACAGTTGGGTTCTCCCCAGGGAGGAAGCACAAGGCTGGATGGGCCAGGCTGT
GCAGGGAGGGACCAGGACAAGCAGGTCCCATGCATCATTCCCCAAGCTGGAGCTGGGCCTGGGACACCGT
CCCTCCCCAACCCGGGAGCCGCCCACCTGCTCCATCTGTCTGGAAAGGCTTCGAGAGCCCATCTCGCTGG
ACTGTGGCCATGACTTCTGCATCCGATGCTTCAGCACACACCGCATCCCAGGCTGTGAGCTACCATGCTG
TCCTGAATGCCGGAAGATCTGTAAGCAAAGGAAGGGCCTCCGCAGTCTAGGGGAAAGGATGAAACTCCTA
CCACAGCGGCCACTGCCCCCTGCACTGCAGGAGACCTGTGCTGTGAGGGCAGAACGTCTGCTGTTGGTAC
GAATCAATGCCTCCGGAGGCCTCATCCTCAGGATGGGCGCCATAAACCGTTGCCTGAAGCATCCACTGGC
CAGGGACACACCCGTCTGCTTATTGGCTGTCCTGGGAGAGCAGCACTCAGGAAAGTCCTTCCTTTTGGAC
CACTTGCTCAGTGGCTTACCAAGCCTGGAATCTGGTGACAGCGGCAGGCCCAGAGCAGAGGGGTCTCTGC
CTGGGATCAGATGGGGCGCTAACGGCCTCACGAGGGGTATCTGGATGTGGAGTCACCCCTTCCTGCTGGG
AAAAGAGGGGAAGAAGGTGGCTGTGTTCTTAGTGGACACGGGGGATGTCATGAGCCCAGAACTGAGCAAG
GAGACAAGGGTCAAGCTCTGTGCTCTCACCATGATGCTCAGTTCCTACCAGATCCTGAATACCTCCCAAG
AGCTGAAGGATACCGATCTGGGCTATCTAGAGATGTTCGTTCACGTGGCTGAGGTGATGGGCAAACATTA
TGGGATGGTACCAATCCAGCATCTGGATCTCTTAGTCCGAGACTCTTCCCATCATAATAAGTCAGGGCAA
GGGCACGTGGGTGACATACTCCAGAAGCTGTCCGGCAAATACCCCAAGGTCCAGGAGCTGCTCCTCGGGA
AACGGGCCCGCTGTTACCTCCTCCCTGCTCCTGAGAGGCAGTGGGTAAACAAAGACCAAGCCAGCCCCAG
AGGCAACACAGAAGATGACTTCTCCCACCATTTCCGGGCCTACATCTTGGACGTGCTGAGCACAGCCCCT
CAGCACGCTAAGAGCCGCTGTCAAGGGTACTGGAGTGAGGGGCGTGCCGTGGCCAGGGGAGACAGACGCC
TGCTCACAGGGCAGCAGCTAGCACAGGAGATCAAGAATCTCTCTGGCTGGATGGGGAAGACTGGGCCCAG
TTTCAACTCTCCTGATGAGATGGCTGCTCAGCTCCATGATCTGAGGAAAGTGGAAGCCGCCAAGAAGGAG
TTCGAGGAGTACGTGAGACAGCAGGACATAGCCACCAAGCGCATCTTCTCTGCACTACGAGTCCTGCCTG
ACACGATGAGGAACCTCCTCTCTACCCAGAAGGATGCCATCTTGGCCCGCCATGGTGTGGCCCTGTTGTG
CAAGGAGAGAGAGCAGACCTTGGAGGCCCTGGAAGCCGAGCTGCAGGCAGAAGCCAAGGCCTTCATGGAC
TCCTACACAATGCGCTTCTGTGGCCACCTGGCCGCGGTAGGGGGCGCTGTAGGTGCTGGGCTCATGGGCC
TGGCAGGGGGTGTGGTGGGCGCAGGCATGGCAGCAGCGGCGTTGGCTGCTGAGGCTGGGATGGTAGCAGC
TGGGGCCGCGGTGGGGGCCACCGGGGCTGCTGTGGTTGGGGGTGGTGTGGGTGCTGGTCTGGCCGCCACT
GTGGGCTGCATGGAGAAAGAGGAAGATGAGAGAGTTCAAGGAGGGGACCGAGAGCCCCTACTCCAGGAGG
AA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209790 protein sequence
Red=Cloning site Green=Tags(s)

MPRPVLSVTAFCHRLGKRESKRSFMGNSSNSWVLPREEAQGWMGQAVQGGTRTSRSHASFPKLELGLGHR
PSPTREPPTCSICLERLREPISLDCGHDFCIRCFSTHRIPGCELPCCPECRKICKQRKGLRSLGERMKLL
PQRPLPPALQETCAVRAERLLLVRINASGGLILRMGAINRCLKHPLARDTPVCLLAVLGEQHSGKSFLLD
HLLSGLPSLESGDSGRPRAEGSLPGIRWGANGLTRGIWMWSHPFLLGKEGKKVAVFLVDTGDVMSPELSK
ETRVKLCALTMMLSSYQILNTSQELKDTDLGYLEMFVHVAEVMGKHYGMVPIQHLDLLVRDSSHHNKSGQ
GHVGDILQKLSGKYPKVQELLLGKRARCYLLPAPERQWVNKDQASPRGNTEDDFSHHFRAYILDVLSTAP
QHAKSRCQGYWSEGRAVARGDRRLLTGQQLAQEIKNLSGWMGKTGPSFNSPDEMAAQLHDLRKVEAAKKE
FEEYVRQQDIATKRIFSALRVLPDTMRNLLSTQKDAILARHGVALLCKEREQTLEALEAELQAEAKAFMD
SYTMRFCGHLAAVGGAVGAGLMGLAGGVVGAGMAAAALAAEAGMVAAGAAVGATGAAVVGGGVGAGLAAT
VGCMEKEEDERVQGGDREPLLQEE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009548
ORF Size 1962 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009548.3
RefSeq Size 3098 bp
RefSeq ORF 1965 bp
Locus ID 22671
UniProt ID Q96DY5
Cytogenetics 11 37.96 cM
MW 71.3 kDa
Summary E3 ubiquitin-protein ligase that plays an important role in neuronal differentiation, including neurogenesis and gliogenesis, during brain development. During embryonic development initiates neuronal differentiation by inducing cell cycle arrest at the G0/G1 phase through up-regulation of cell-cycle regulatory proteins (PubMed:21566658, PubMed:28684796). Plays a role not only in the fetal period during the development of the nervous system, but also in the adult brain, where it is involved in the maintenance of neural functions and protection of the nervous tissue cells from oxidative stress-induced damage (PubMed:27918959, PubMed:26792191, PubMed:26951452). Exhibits GTPase and E3 ubiquitin-protein ligase activities. Regulates dendritic spine density and synaptic neurotransmission; its ability to hydrolyze GTP is involved in the maintenance of dendritic spine density (PubMed:26212327).UniProtKB/Swiss-Prot Function
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"NM_009548" in other vectors (4)
SKU Description Size Price
MC201184 Rnf112 (untagged) - Mouse ring finger protein 112 (Rnf112), (10ug) 10 ug
$610.00
MG209790 Rnf112 (tGFP-tagged) - Mouse zinc finger protein 179 (Zfp179) 10 ug
$810.00
MR209790L3 Lenti ORF clone of Rnf112 (Myc-DDK-tagged) - Mouse ring finger protein 112 (Rnf112) 10 ug
$910.00
MR209790L4 Lenti ORF clone of Rnf112 (mGFP-tagged) - Mouse ring finger protein 112 (Rnf112) 10 ug
$910.00

Plasmid Map for MR209790

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209790
Circular map for MR209790

file-alt Citations

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