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Dars2 (NM_172644) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209779
Dars2 (Myc-DDK-tagged) - Mouse aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $609.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Dars2 or NM_172644"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dars2
Synonyms 5830468K18Rik; aspRS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209779 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATCTTGGTTTTTGGTTAAGTCGTCTATGCAGGGGTTTATCCAGACCCATCGGGAAGACCATGCGGC
CAATCTGGGGTTCTCTTTCCAGAAATCTGGCGTTGAGTTCACAAAGAATTCCAGAATTCAGTAGCTTTGT
TGCTCGGACCAACACATGTGGAGAGTTGCGTTCGTCCCACTTAGGCCAAGAAGTCACTCTGTGTGGATGG
GTTCAGTACCGCAGGCAGAACACCTTCTTAGTCCTAAGAGATTGCCACGGCCTTGTTCAGATTCTCATTC
CCCAGGATGAGTCAGCCGCATCTGTGAGGGGGATTTTGTGTGAAGCCCCTGTGGAGTCTGTGGTGAGAGT
ATCTGGGACAGTAATCTCCCGTCCTCCAGGACAAGAGAATCCGAAAATGCCGACGGGTGAGATTGAAATC
AAAGTTAAAACAGCTGAGCTCCTGAATGCCTGCAAAAAGCTGCCCTTTGAGATTAAAGACTTTGTGAAGA
AAACAGAGGCGCTCCGTTTGCAGTATCGCTACTTAGATCTGCGCAGCTTCCAGATGCAGTACAACTTGCG
GCTGAGGTCGCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCACGGGTTTGTGGATATAGAA
ACCCCAACCTTGTTTAAGAGGACCCCTGGAGGTGCCAAGGAGTTTTTAGTACCATCCAGGGAACCTGGAA
AGTTTTATTCCCTCCCTCAAAGTCCTCAGCAATTTAAGCAGCTTCTGATGGTTGGAGGTTTAGACAGGTA
TTTTCAGGTCGCTCGGTGTTACCGAGATGAAGGCTCAAGACCGGATAGACAGCCAGAGTTCACTCAGATT
GACATAGAGATGTCCTTTGTAGAGCAGACTGGCATTCAGCGTTTAGTTGAGGGTTTGCTGCAGTATTCCT
GGCCTGGTGATAAAGACCCTCTAGTGACTCCTTTCCCTTCCATGACTTTTGCTGAGGCACTGGCCACCTA
TGGAACTGATAAGCCAGATACTCGCTTTGGGATGAAGATTGTGGATGTCAGTGATGTGTTCAGAAACACA
GAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACG
GAGCGAAATACTTAAGGAAGGAAGACATTGAGTTCATTAGGAAGTTTGCAGTTCACCATTTCAGTCAGGA
AGTCCTACCTATATTCCTGAATGCCAAGAAGAACTGGAGTTCGCCATTTGCTAAGTTCATAATGGAGGAG
GAAAGACTGGAATTGGCCAGATCGATGGAGATACAAGAAGAAGACATAGTGTTACTGACTGCTGGGGAAC
ATGAGAAAGCATGCTCTCTGTTGGGAAAGTTACGGCTAGAGTGTGCAGACCTTTTGGAGATGAGAGGAGC
GGTGCTCCGAGACCCAGCAGTGTTCTCTTTTCTTTGGGTGGTTGACTTTCCACTCTTCCTTGCCAAGGAA
GAGAGTCCCACAGAGCTGGAGTCAGCCCATCACCCATTTACTGCTCCTAACTCCAGTGACATTCACCTCC
TCTATACTGAGCCGGAAAAGGTTCGTGGCCAACACTATGACTTGGTTTTAAACGGCAATGAGATAGGAGG
TGGTTCTGCCCGAATCCATGATGCACAGCTACAGAGGTACATCCTGGAAACACTGCTAAAGGAAGATGTG
AAATTGCTTTCCCATCTGCTTCAGGCTTTAGACTATGGGGCACCCCCACACGGAGGAATTGCCTTAGGGT
TAGACAGACTGGTATGTCTTGTCACGGGAGCACCCAGCATCAGAGATGTCATAGCCTTCCCTAAATCCTA
CCGAGGACAGGACCTTATGAGCAATGCTCCAGACTCCGTGTCTCCAGAAGAGCTGAAGCCTTACCATATC
CACGTCTTGTGGCCAGCAGACTCAGAAGAGGAGAGTGCATCCGCCACACCTTCCAAACACTTGAGCTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209779 protein sequence
Red=Cloning site Green=Tags(s)

MYLGFWLSRLCRGLSRPIGKTMRPIWGSLSRNLALSSQRIPEFSSFVARTNTCGELRSSHLGQEVTLCGW
VQYRRQNTFLVLRDCHGLVQILIPQDESAASVRGILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEI
KVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIE
TPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQI
DIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNT
ELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEE
ERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKE
ESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSARIHDAQLQRYILETLLKEDV
KLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHI
HVLWPADSEEESASATPSKHLSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172644
ORF Size 1959 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172644.1, NM_172644.2, NM_172644.3, NM_172644.4, NP_766232.1
RefSeq Size 3581 bp
RefSeq ORF 1962 bp
Locus ID 226539
UniProt ID Q8BIP0
Cytogenetics 1 H2.1
MW 74 kDa
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Documents
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"NM_172644" in other vectors (4)
SKU Description Size Price
MC203432 Dars2 (untagged) - Mouse aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$609.00
MG209779 Dars2 (tGFP-tagged) - Mouse aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2) 10 ug
$809.00
MR209779L3 Lenti ORF clone of Dars2 (Myc-DDK-tagged) - Mouse aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), nuclear gene encoding mitochondrial protein 10 ug
$909.00
MR209779L4 Lenti ORF clone of Dars2 (mGFP-tagged) - Mouse aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), nuclear gene encoding mitochondrial protein 10 ug
$909.00

Plasmid Map for MR209779

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209779
Circular map for MR209779

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