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Vit (NM_028813) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209763
Vit (Myc-DDK-tagged) - Mouse vitrin (Vit), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $606.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vit
Synonyms 1700052E02Rik; 1700110E08Rik; 2810429K11Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209763 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAATTGTTGTTCCCACTATGAAGGCATCTGTTATTGAAGTGTTGCTTGTTTTGCTGGTAACTGGAA
TACATTCAAATAAAGAAACGCCAAAGAAGACTAAAAGGCCCAAACTCACTGTGCCTCAGATCAACTGCGA
TGTCAAAGCTGGAAAGATCATCAATCCTGAGTTCATGGTGAAATGTCCAGCGGGCTGCCAGGACCCCAAA
TACCATGTTTACGGCACTGGCGTCTACGCGTCTTACTCCAGCGTGTGTGGCGCCGCAATTCACAGCGGTG
TGCTGGATAATTCAGGAGGGAAAATACTTGTTCGGAAAGTTGCTGGGCAGTCTGGTTACAAAGGAAGCTA
CTCGAATGGGGTTCAGTCTTTATCTTTACCCAGATGGCGAGAGTCCTTCATCGTCGCAGAAAGTAAGCCC
CAAAAGGGTGTAGCCTACCCATCGACTCTTACATATTCATCATCGAAAACTGCAGCTGCTAAAGCAGGTG
AGACCACAAAAGCCTATGAGAAGCCATCTATTCCAGGGACAACCATACAGCCTGTCACCCTGACACAGGC
TCAAGCTACCCCTGTAGCTGAGGTCACCCACAGATCCACCTCAAAGCCATTTGCAGCATCTGTCACCAAC
AGCCCCAGACCCCAACCTGTGGGCCACAGGAGTCAGGAGATGGAAGAGGTGGACGGGTGGAAGCCTGGAC
CAGTCCTTTTAGATTCAGGATTTGTGCCAAAAGAAGAACTCAGCACACAGTCTTCGGAGCCAGTGCCGCA
GGGAGATCCAAACTGCAAAATTGACTTGTCCTTCTTAATTGATGGGAGCACCAGCATCGGCAAGCGCCGC
TTCCGGATCCAGAAGCAGTTCCTAGCGGACGTCGTCCAGGCTCTTGACATCGGCCCTGCCGGTCCCCTCG
TGGGTGTTGTTCAGTACGGAGACAATCCTGCTACCCAGTTTAACCTCAAAACTCACATGAATTCTCAAGA
TCTGAAGACAGCTATAGAGAAAATTACCCAGAGAGGAGGCCTTTCTAATGTAGGCCGTGCCATCTCCTTT
GTAACCAAGACCTTCTTTTCCAAAGCCAATGGGAACAGAGGTGGTGCTCCCAATGTGGCTGTGGTCATGG
TGGATGGCTGGCCCACAGACAAGGTGGAAGAGGTGTCACGAGTTGCCAGAGAGTCTGGGATCAACGTCTT
CTTCATCACCGTCGAAGGGGCCGCCGAAAGGGACATACAGCATGTGGTGGAGCCCGGTTTTGCCAGCAAG
GCAGTGTGCAGAACAAACGGGTTCTACTCCTTCAATGTGCAGAGCTGGCTCAGCCTTCACAAAACAGTGC
AGCCCCTGGTGAAGCGGGTCTGCGACACGGACCGCCTAGCCTGCAGCAAGACCTGTTTAAACTCTGCGGA
CATAGGCTTTGTCATCGATGGGTCTAGCAGCATGGGGACAAGCAACTTCCGCACGGTCCTGCAGTTCGTG
GCCAACCTCAGCAAGGAGTTTGAGATTTCAGACACCGACACACGCGTCGGGGCCGTGCAGTACACCTACG
AGCAGCGGCTCGAATTTGGGTTCGACAAGTATAACAGCAAAGCCGACATCCTCAGTGCCATCAGGAGGGT
GGGGTACTGGAGCGGAGGCACCAGCACAGGGGCTGCCATCCAGTATGCCCTCGAACAGCTCTTCAAGAAG
TCCAAACCGAACAAGAGGAAGGTAATGATCATTATCACTGACGGCAGGTCTTACGATGACGTGCGGATCC
CAGCCATGGCTGCCTACCAGAAGGGGGTGATCACGTATGCAATAGGCATTGCGTGGGCTGCTCAGGACGA
GCTGGAGGTAATGGCCACTCACCCCGCCAAGGACCATTCCTTCTTTGTGGATGATTTTGACAACCTCTAC
AAAATTGCCCCCAGGATCATCCAGAATATTTGTACAGAGTTCAACTCACAGCCTCGAAAC

ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209763 protein sequence
Red=Cloning site Green=Tags(s)

MGIVVPTMKASVIEVLLVLLVTGIHSNKETPKKTKRPKLTVPQINCDVKAGKIINPEFMVKCPAGCQDPK
YHVYGTGVYASYSSVCGAAIHSGVLDNSGGKILVRKVAGQSGYKGSYSNGVQSLSLPRWRESFIVAESKP
QKGVAYPSTLTYSSSKTAAAKAGETTKAYEKPSIPGTTIQPVTLTQAQATPVAEVTHRSTSKPFAASVTN
SPRPQPVGHRSQEMEEVDGWKPGPVLLDSGFVPKEELSTQSSEPVPQGDPNCKIDLSFLIDGSTSIGKRR
FRIQKQFLADVVQALDIGPAGPLVGVVQYGDNPATQFNLKTHMNSQDLKTAIEKITQRGGLSNVGRAISF
VTKTFFSKANGNRGGAPNVAVVMVDGWPTDKVEEVSRVARESGINVFFITVEGAAERDIQHVVEPGFASK
AVCRTNGFYSFNVQSWLSLHKTVQPLVKRVCDTDRLACSKTCLNSADIGFVIDGSSSMGTSNFRTVLQFV
ANLSKEFEISDTDTRVGAVQYTYEQRLEFGFDKYNSKADILSAIRRVGYWSGGTSTGAAIQYALEQLFKK
SKPNKRKVMIIITDGRSYDDVRIPAMAAYQKGVITYAIGIAWAAQDELEVMATHPAKDHSFFVDDFDNLY
KIAPRIIQNICTEFNSQPRN

TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-NotI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028813
ORF Size 1950 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028813.2, NP_083089.1
RefSeq Size 2737 bp
RefSeq ORF 1953 bp
Locus ID 74199
UniProt ID Q8VHI5
Cytogenetics 17 E3
MW 70.7 kDa
Summary Promotes matrix assembly and cell adhesiveness (PubMed:18757743). Plays a role in spinal cord formation by regulating the proliferation and differentiation of neural stem cells (PubMed:25331329).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028813" in other vectors (4)
SKU Description Size Price
MC220057 Vit (untagged) - Mouse vitrin (Vit), transcript variant 1, (10ug) 10 ug
$665.00
MG209763 Vit (tGFP-tagged) - Mouse vitrin (Vit) 10 ug
$806.00
MR209763L3 Lenti ORF clone of Vit (Myc-DDK-tagged) - Mouse vitrin (Vit), transcript variant 1 10 ug
$906.00
MR209763L4 Lenti ORF clone of Vit (mGFP-tagged) - Mouse vitrin (Vit), transcript variant 1 10 ug
$906.00

Plasmid Map for MR209763

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209763
Circular map for MR209763

file-alt Citations

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