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Wac (NM_153085) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209727
Wac (Myc-DDK-tagged) - Mouse WW domain containing adaptor with coiled-coil (Wac), transcript variant 1
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $602.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Wac or NM_153085"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wac
Synonyms 1110067P07Rik; A230035H12Rik; AI256735; AI256776; Wwp4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209727 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAATGTATGCGAGGAAACAGCAGAGACTCAGTGATGGCTGTCACGACCGGAGAGGGGACTCTCAGC
CTTTCCAGGCACTTAAGTATTCTTCAAAGAGTCACCCCAGTAGTGGTGATCACAGACATGAAAAGATGCG
AGACGCCGCAGATCCTTCACCACCAAACAAAATGTTGCGGAGATCTAATAGTCCTGAAAACAAATACAGT
GACAGCACAGGTCACAACAAGGCCAAAAACGTGCACACTCAGAGAGTTAGAGAAAGGGACGGTGGGACCA
GTTACTCTCCACAAGAAAACTCACACAACCACAGTGCACTTCATAGTTCAAATTCACATTCTTCTAATCC
AAGCAATAATCCAAGCAAAACTTCAGATGCACCTTATGATTCTGCAGATGATTGGTCTGAGCACATTAGC
TCATCTGGAAAAAAGTACTACTACAATTGTCGCACAGAAGTTTCACAGTGGGAGAAACCAAAAGAGTGGC
TTGAAAGAGAACAGAGACAAAAAGAAGCAAATAAGCTGGCAGTTAATAGTTTCCCAAAAGACAGGGATTA
CAGAAGAGAGGTGATGCAAGCAACAGCCACTAGTGGGTTCACCAGTGGAATGGAAGACAAGCATTCCAGC
GATGCCAGTAGTTTGCTCCCACAGAATATTTTGTCTCAAACAAGCAGACACAATGACAAAGACTACAGAC
TGCCAAGAGCAGAGACTCACAGTAGCTCTACGCCAGTACAGCACCCCATCAAACCAGTGGTTCATCCAAC
CGCTACCCCAAGCACTGTTCCTTCTAGTCCATTTACGCTTCAGTCTGATCACCAGCCAAAGAAATCATTT
GATGCTAATGGAGCATCTACTTTATCCAAACTGCCTACACCCACAGCTTCTCTCCCTGCACAGAAAACAG
AGAGAAAAGAATCTGCTCCAGGAGACAAATCAATATCACATTCTTGCACAACTCCTTCCACATCTTCAGC
CTCTGGCCTGAACCCTACATCTGCGCCTCCGACATCTGCTTCAGCAGTACCTGTTTCTCCCGTTCCACAA
TCAACAATACCTCCATTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGCCACGC
TACAGCTTAATAATTCTAATGTGGACATATCCAAAATAAATGAAGTTCTTACAGCAGCTGTAACACAAGC
TTCCCTGCAGTCTATCATCCACAAGTTTCTTACTGCTGGACCATCTGCTTTCAACATCACCTCACTGATT
TCTCAAGCTGCCCAGCTTTCTACACAAGCCCAGCCATCTAATCAGTCTCCAATGTCTCTGACATCTGATG
CTTCATCCCCAAGATCCTATGTGTCTCCAAGAATAAGCACACCTCAAACTAATACAGTCCCTATGAAACC
TTTGATTAGTACTCCACCTGTTTCATCCCAGCCAAAGGTTAGTACTCCAGTAGTTAAGCAAGGACCAGTG
TCACATTCGGCTACACAGCAGCCTGTAACTGCTGACAAGCAGCAGAGCCATGATTCTGTTTCTCCTCGAA
GTCTTCAGCGCTTAAGTAGCCAGAGAAGTCCATCACCTGGTCCAAATCATACTTGTAGTAGTAATGCATC
AACTGCAACTGTTGTACCACAGAATGCATCTGCTAGACCTGCGTGCTCATTAACACCTACTCTAGCAGCG
CACTTCAATGATAATCTCATAAAGCATGTTCAAGGGTGGCCTGCAGACCACGCAGAGAAACAGGCATCAA
GATTGCGTGAAGAAGCCCATAATATGGGAAGTGTACATATGTCAGAAATATGTACTGAGTTAAAAAATTT
GAGATCATTAGTTCGAGTATGTGAAATTCAAGCAACTTTGCGAGAGCAGAGGATACTATTTTTGAGACAA
CAAATTAAGGAACTCGAAAAGCTGAAAAATCAGAATTCCTTCATGGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209727 protein sequence
Red=Cloning site Green=Tags(s)

MVMYARKQQRLSDGCHDRRGDSQPFQALKYSSKSHPSSGDHRHEKMRDAADPSPPNKMLRRSNSPENKYS
DSTGHNKAKNVHTQRVRERDGGTSYSPQENSHNHSALHSSNSHSSNPSNNPSKTSDAPYDSADDWSEHIS
SSGKKYYYNCRTEVSQWEKPKEWLEREQRQKEANKLAVNSFPKDRDYRREVMQATATSGFTSGMEDKHSS
DASSLLPQNILSQTSRHNDKDYRLPRAETHSSSTPVQHPIKPVVHPTATPSTVPSSPFTLQSDHQPKKSF
DANGASTLSKLPTPTASLPAQKTERKESAPGDKSISHSCTTPSTSSASGLNPTSAPPTSASAVPVSPVPQ
STIPPLLQDPNLFRQLLPALQATLQLNNSNVDISKINEVLTAAVTQASLQSIIHKFLTAGPSAFNITSLI
SQAAQLSTQAQPSNQSPMSLTSDASSPRSYVSPRISTPQTNTVPMKPLISTPPVSSQPKVSTPVVKQGPV
SHSATQQPVTADKQQSHDSVSPRSLQRLSSQRSPSPGPNHTCSSNASTATVVPQNASARPACSLTPTLAA
HFNDNLIKHVQGWPADHAEKQASRLREEAHNMGSVHMSEICTELKNLRSLVRVCEIQATLREQRILFLRQ
QIKELEKLKNQNSFMV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153085
ORF Size 1938 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153085.2
RefSeq Size 5217 bp
RefSeq ORF 1941 bp
Locus ID 225131
UniProt ID Q924H7
Cytogenetics 18 A1
MW 70.7 kDa
Summary Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. Also acts as a negative regulator of basal autophagy. Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation. May negatively regulate the ubiquitin proteasome pathway.UniProtKB/Swiss-Prot Function
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"NM_153085" in other vectors (2)
SKU Description Size Price
MR209727L3 Lenti ORF clone of Wac (Myc-DDK-tagged) - Mouse WW domain containing adaptor with coiled-coil (Wac), transcript variant 1 10 ug
$902.00
MR209727L4 Lenti ORF clone of Wac (mGFP-tagged) - Mouse WW domain containing adaptor with coiled-coil (Wac), transcript variant 1 10 ug
$902.00

Plasmid Map for MR209727

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209727
Circular map for MR209727

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