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Melk (NM_010790) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209713
Melk (Myc-DDK-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $599.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Melk
Synonyms AI327312; mKIAA0175; MPK38
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209713 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGATTATGACGAACTCCTTAAATACTATGAACTATATGAAACGATTGGGACAGGTGGCTTTGCAA
AGGTCAAACTGGCCTGCCATGTCCTCACTGGAGAGATGGTAGCTATAAAAATCATGGATAAGAATGCGCT
AGGGAGTGATTTGCCCCGAGTCAAAACTGAGATCGATGCGCTGAAGAGTCTGAGACATCAGCACATATGT
CAGCTCTACCATGTGCTGGAGACAAAGAACAAAATATTCATGGTTCTGGAGTACTGTCCAGGAGGAGAGC
TGTTTGACTACATAATCTCCCAGGATCGCCTGTCGGAAGAGGAGACCCGGGTCGTCTTCCGTCAGATACT
GTCTGCAGTTGCGTATGTCCACAGCCAGGGCTATGCCCACAGGGACCTCAAACCAGAAAATTTATTATTT
GATGAAAATCATAAGCTAAAGCTGATTGACTTTGGTCTTTGTGCAAAACCCAAGGGCAACAAGGACTACC
ATCTGCAGACGTGCTGTGGGAGCCTTGCTTATGCAGCTCCTGAACTAATACAAGGGAAGTCGTACCTTGG
ATCAGAGGCAGATGTTTGGAGCATGGGCATCCTCCTGTATGTGCTCATGTGTGGATTTCTACCATTTGAT
GATGATAATGTCATGGCTTTGTACAAGAAGATAATGAGAGGGAAATACGAAGTTCCTAAGTGGCTCTCTC
CCAGTAGCATTCTGCTTCTCCAGCAGATGTTGCAGGTGGACCCAAAGAAACGGATTTCTATGAGAAATCT
CCTGAACCATCCCTGGGTCATGCAAGATTACAGCTGTCCCGTGGAGTGGCAAAGCAAGACTCCTTTGACT
CACCTCGATGAGGATTGCGTGACAGAGCTTTCTGTACATCACCGCAGCAGCAGGCAGACAATGGAGGATT
TAATTTCGTCGTGGCGGTACGATCACCTCACAGCCACCTACCTTCTGCTTCTAGCCAAGAAGGCCCGGGG
GAAGCCGGCTCGTCTACAGCTCCTGTCCTTCTCTTGTGGAACCGCCAGCACCACTCCAAAGTCAAAGAAT
CTGAGCCTGGAAGATATGAGCACAAGTGATGATAACTGTGTGGCTGGATTGATAGACTATGAATTGTGTG
AAGATAAATTATTAGCTCCCAAGACGCCACAGGTTACCAAACACTTGGCAGAATCAAATCACGCAGCATC
TAAATCACCAGCGCCAGGGGTACGCAGAGCAGTGGCAAATAAATTAATGGACAAAGAAAATGTGTGCACT
CCCAAGTCTTCTGTGAAGAATGAAGAGCAGTTTGTATTTTCTGAGCCGAAGATTCCAGTTAGTAAGAACC
AGTATAAGAGAGAAATACCCGCCTCACCAACCCGTTTCCCAACACCTGCAAAAGCTAGAGCCCAGTGCCT
GAGAGAAGCCCCGGTTAGAACACCAGGGAATTCCGCAGGAGCAGACACACTAACGACAGGTGTCATTAGC
CCCGAGAGGAGGTGCCGTTCAATGGACGTGGATCTCAACCAGGCACACATGGAGGATACCCCGAAAAAGA
AAGGAACCAATGTGTTTGGGAGCCTTGAGAGAGGACTGGATAAGGTTCTCACTGCGCTCACAAGGAACAA
GAAGAAGGGCTCTGCCAGAGATGGACCAAGAAAGCGAAAGCTGCACTACAATGTGACTACAACTCGCCTG
GTGAACCCCGACCAGCTCCTGAGCGAAATCATGGCTATTCTTCCAAAGAAGAACGTGGACTTCGTACAGA
AAGGTTACACTCTAAAGTGTCAAACGCAGTCTGATTTTGGCAAAGTGACAATGCAGTTTGAACTGGAAGT
GTGCCAGCTGCAGAGACCTGACGTGGTAGGCATCCGGAGACAGCGGCTGAAGGGTGATGCCTGGGTTTAC
AAGAGATTAGTGGAAGATATCTTGTCTGGCTGCAAGATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209713 protein sequence
Red=Cloning site Green=Tags(s)

MKDYDELLKYYELYETIGTGGFAKVKLACHVLTGEMVAIKIMDKNALGSDLPRVKTEIDALKSLRHQHIC
QLYHVLETKNKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQILSAVAYVHSQGYAHRDLKPENLLF
DENHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFD
DDNVMALYKKIMRGKYEVPKWLSPSSILLLQQMLQVDPKKRISMRNLLNHPWVMQDYSCPVEWQSKTPLT
HLDEDCVTELSVHHRSSRQTMEDLISSWRYDHLTATYLLLLAKKARGKPARLQLLSFSCGTASTTPKSKN
LSLEDMSTSDDNCVAGLIDYELCEDKLLAPKTPQVTKHLAESNHAASKSPAPGVRRAVANKLMDKENVCT
PKSSVKNEEQFVFSEPKIPVSKNQYKREIPASPTRFPTPAKARAQCLREAPVRTPGNSAGADTLTTGVIS
PERRCRSMDVDLNQAHMEDTPKKKGTNVFGSLERGLDKVLTALTRNKKKGSARDGPRKRKLHYNVTTTRL
VNPDQLLSEIMAILPKKNVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQRPDVVGIRRQRLKGDAWVY
KRLVEDILSGCKM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010790
ORF Size 1929 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010790.1, NM_010790.2, NP_034920.2
RefSeq Size 2914 bp
RefSeq ORF 1932 bp
Locus ID 17279
UniProt ID Q61846
Cytogenetics 4 23.46 cM
MW 72.8 kDa
Summary Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.UniProtKB/Swiss-Prot Function
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"NM_010790" in other vectors (4)
SKU Description Size Price
MC219961 Melk (untagged) - Mouse maternal embryonic leucine zipper kinase (Melk), (10ug) 10 ug
$659.00
MG209713 Melk (tGFP-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk) 10 ug
$799.00
MR209713L3 Lenti ORF clone of Melk (Myc-DDK-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk) 10 ug
$899.00
MR209713L4 Lenti ORF clone of Melk (mGFP-tagged) - Mouse maternal embryonic leucine zipper kinase (Melk) 10 ug
$899.00

Plasmid Map for MR209713

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209713
Circular map for MR209713

file-alt Citations

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