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Cog8 (NM_139229) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209686
Cog8 (Myc-DDK-tagged) - Mouse component of oligomeric golgi complex 8 (Cog8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $596.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cog8
Synonyms BB235941; C87832
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209686 representing NM_139229
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGCTCTCCCTCTCCAGCCCTCCACGACCGCTTCGGCCACGACGACAGCCACGGCCGTCGCGC
TGGGGGAGGTGGAAGATGAGGGCCTCCTGGCGTCGCTATTCCGGGACCGCTTCCCCGAAGCTCAGTGGCG
TGAGAAGCCCGACGTGGGCCGCTACCTCCGGGAGCTGAGCGGCTCCGGGCTGGACCGGCTGCGGCGCGAA
CCCGAGCGCTTGGCGGAAGAGCGGGCCCAGCGGCTGCAGCAGACGCGCGACCTGGCTTTCGCCAACTACA
AGACGTTCATTCGCGGGGCCGAGTGCACCGAGCGCATCCACCGGCTCTTCGGCGACGTGGAGGCGTCCCT
CGGGCGCCTGCTCGACCGTCTGCCCCGCTTCCAGCAGAGCTGCAGGAACTTTGTGAAGGAAGCTGAGGAG
ATCAGCTCCAGCCGCCGCATGAACACGCTGACTCTAAACCGGCACACAGAAATCCTGGAGATTTTAGAGA
TACCCCAGCTTATGGACACGTGTGTCCGAAACAGCTATCATGAAGAGGCGCTGGAGCTTGCCGCCTATGT
ACGGCGACTGGAAAGGAAGTACTCCTCCATCCCCGTTATCCAGGGCATTGTGAATGAAGTGCGCCAGTCC
ATGCAGCTGATGCTAAGCCAGCTGATCCAACAACTGAGGACTAACATCCAGCTCCCAGCCTGCCTCCGTG
TCATAGGCTATCTTCGGCGCATGGATGTCTTCACTGAGGCTGAGTTGAGGGTGAAATTTCTTCAGGCTCG
GGATGCTTGGCTCCGCTCCATCCTGACTGCCATCCCTAATGATGATCCTTACTTCCACATCACAAAAACC
ATTGAGGCTTGCCGGGTCCATCTCTTTGATATCATCACCCAGTACCGTGCTATTTTCTCAGATGAGGACC
CCCTGCTGCCACCTGCCATGGGCGAGTACACTGTGAATGAGGGTGCCATCTTCCATGGATGGGTGCTGCA
GAAAATCTCACAGTTCCTGCAGGTGCTAGAGACTGACCTTTACCGGGGTATAGGAGGCCGCTTGGACTCT
CTGCTTGGCCAGTGCATGTACTTTGGGCTGTCCTTTAGCCGGGTGGGGGCTGATTTCCGCGGCCAGTTGG
CTCCTGTTTTCCAGAGGGTGGCAATCAGCACTTTCCAGAAAGCAGTTGAAGAAGCAGTGGAGAAATTCCA
GGATGAAATGACTTCATACACCCTCATCTCGACTGCAGCCATCCTGGGCAGCAGTAACACGCCTGCCACA
GTACCAGCCACACAACCAGGCACGCTGCAGCCACCGATGGTGCTCTTAGACTTCCCGCCTCTTGCCTGTT
TCCTCAACAACATTTTGGTTGCTTTCAATGATTTACGTCTCTGTTGCCCTGTGGCTCTAGCACAGGATGT
GACTGGGACCTTGGAAAATGCCCTTACCAAGGTAACCAAAACAATTCTGGCATTCCATCGAGCCGAAGAG
GCTGTGTTCAGCAGTGGGGAGCATGAGATCTTTGTCCAGTTCTGCACTGCCTTCCTGGAAGACCTTGTTC
CTTATTTGAACCGCTGTCTTCAAGTCCTTTTCCCACCAGCTCAGATAGCCCAGACTTTAGGCATTTCACC
CACCCAGCTCTCTAAGCACGGAAACCTTGGACACGTGAACATCAGCGCCATCCAGGAACCTCTCGCCTTT
ATTTTGCCTAAGAGAGAAACAGTTTTCTGTTTAGATGAGCAGGAGCTGGGGCCGGACCTCGTGGCCCCAG
CTCCAGAGCTCCCTGCGGAGCAGCGGAGCATGGAGCCTGTAACAGAGAAGAGGGAACCAGGGGAGCCGCT
GCCTCAGGAGCCCATGGAGGGGGAGCCGCTGCCAGCCGAGCCACCGAGTGAGGGAGGAGCCATAGGCAGT
GTCCCCTGCCCGCAGCCTGGGGAGCAGCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209686 representing NM_139229
Red=Cloning site Green=Tags(s)

MAAALPLQPSTTASATTTATAVALGEVEDEGLLASLFRDRFPEAQWREKPDVGRYLRELSGSGLDRLRRE
PERLAEERAQRLQQTRDLAFANYKTFIRGAECTERIHRLFGDVEASLGRLLDRLPRFQQSCRNFVKEAEE
ISSSRRMNTLTLNRHTEILEILEIPQLMDTCVRNSYHEEALELAAYVRRLERKYSSIPVIQGIVNEVRQS
MQLMLSQLIQQLRTNIQLPACLRVIGYLRRMDVFTEAELRVKFLQARDAWLRSILTAIPNDDPYFHITKT
IEACRVHLFDIITQYRAIFSDEDPLLPPAMGEYTVNEGAIFHGWVLQKISQFLQVLETDLYRGIGGRLDS
LLGQCMYFGLSFSRVGADFRGQLAPVFQRVAISTFQKAVEEAVEKFQDEMTSYTLISTAAILGSSNTPAT
VPATQPGTLQPPMVLLDFPPLACFLNNILVAFNDLRLCCPVALAQDVTGTLENALTKVTKTILAFHRAEE
AVFSSGEHEIFVQFCTAFLEDLVPYLNRCLQVLFPPAQIAQTLGISPTQLSKHGNLGHVNISAIQEPLAF
ILPKRETVFCLDEQELGPDLVAPAPELPAEQRSMEPVTEKREPGEPLPQEPMEGEPLPAEPPSEGGAIGS
VPCPQPGEQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139229
ORF Size 1920 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139229.4, NP_631975.3
RefSeq Size 2134 bp
RefSeq ORF 1923 bp
Locus ID 97484
UniProt ID Q9JJA2
Cytogenetics 8 D3
MW 71.6 kDa
Summary Required for normal Golgi function.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_139229" in other vectors (4)
SKU Description Size Price
MC220023 Cog8 (untagged) - Mouse component of oligomeric golgi complex 8 (Cog8), (10ug) 10 ug
$656.00
MG209686 Cog8 (tGFP-tagged) - Mouse component of oligomeric golgi complex 8 (Cog8) 10 ug
$796.00
MR209686L3 Lenti ORF clone of Cog8 (Myc-DDK-tagged) - Mouse component of oligomeric golgi complex 8 (Cog8) 10 ug
$896.00
MR209686L4 Lenti ORF clone of Cog8 (mGFP-tagged) - Mouse component of oligomeric golgi complex 8 (Cog8) 10 ug
$896.00

Plasmid Map for MR209686

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209686
Circular map for MR209686

file-alt Citations

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