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Klhl22 (NM_145479) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209634
Klhl22 (Myc-DDK-tagged) - Mouse kelch-like 22 (Drosophila) (Klhl22)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $591.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klhl22
Synonyms 2610318I18Rik; Kelchl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209634 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGAGCAGGACTTTGCCCAGCTGTGCAGGCTGCCCACACAGCCCTCCCACTCCCACTGTGTCA
ACAATACCTATCGAAGCACACAGCACTCCCAGGCCCTGCTCCGGGGCCTGCTGGCTTTGCGAGACAGTGG
GATCCTCTTTGACGTTGTCCTGGTGGTAGAGGGGAAGCACATTGAGGCCCATCGGATTCTGCTAGCTGCA
TCGTGTGATTATTTCAGAGGAATGTTTGCTGGGGGATTGAAGGAGATGGAGCAGGAAGAGGTCTTGATCC
ACGGTGTGTCTTACAATGCCATGTGCCAAATCCTGCACTTCATATACACTTCTGAACTAGAGCTCAGCCT
GAGCAATGTGCAGGAGACCCTGGTTGCTGCCTGCCAACTTCAGATACCAGAAATTATCCACTTCTGCTGT
GATTTCCTCATGTCATGGGTAGATGAGGAGAATATCCTTGATGTCTACCGGCTAGCAGACCTCTTTGACT
TGAACCATCTGACCCAACAGCTAGATACCTACATCCTCAAAAACTTTGTGGCCTTCTCCAGAACTGACAA
GTACCGCCAGCTTCCCTTGGAGAAGGTCTACTCTCTCCTCAGCAGCAATCGCCTGGAGGTTTCCTGTGAG
ACTGAAGTGTATGAAGGGGCCCTGCTCTACCATTACTCCCTAGAGCAGGTGCAGGCTGACCAGATCTCAC
TGAATGAGCCCCCCAAGCTCCTAGAGACTGTGCGCTTTCCCCTGATGGAAGCTGAGGTCCTTCAGCGGCT
GCATGACAAGCTTGGTCCTAGCCCACTGAGGGACACAGTGGCCAGTGCCCTTATGTACCACCGGAATGAG
ATCCTGCAGCCCAGCCTTCAGGGCCCACAGACAGAGCTTCGCTCGGACTTCCAGTGTGTTGTGGGCTTTG
GGGGTATTCACTCCACACCGTCTACAATCCTCAGTGACCAGGCCAAGTACCTGAATCCTCTACTGGGAGA
GTGGAAACACTTCACTGCCTCTCTGGCGCCCCGAATGTCCAATCAGGGCATTGCAGTGCTCAACAACTTC
GTGTACTTAATTGGAGGGGACAATAATGTCCAAGGATTTCGAGCTGAGTCCCGATGCTGGAGGTATGACC
CAAGGCACAATCGCTGGTTCCAGATCCAGTCCTTGCAGCAAGAACATGCAGACCTATGTGTGTGTGTAGT
GGGCAAGTACATCTATGCTGTGGCAGGCCGTGACTACCACAATGACCTGAGTGCTGTGGAGCGCTATGAC
CCTGCCACCAACTCCTGGGACTATGTGGCCCCACTCAAGAAGGAGGTATATGCCCATGCAGGCACCACAC
TGCAGGGGAAGATGTATATTACCTGTGGTCGCAGAGGAGAGGACTACCTGAAAGAGACACACTGCTATGA
CCCAGGAAGCAACACGTGGCATACTCTGGCTGACGGGCCTGTGCGACGGGCCTGGCATGGCATGGCTGCC
CTCCTCGACAAGCTGTTTGTGATTGGAGGCAGCAACAATGATGCTGGCTACAGAAGGGATGTGCACCAGG
TGGCTTGCTACAGTTGTACATCTCGTCAGTGGTCATCGGTCTGCCCACTCCCAGCTGGGCATGGTGAGCC
TGGCATTGCTGTGCTAGACAGCAGGATATATGTGCTGGGTGGCCGCTCTCACAACCGAGGCAGCCGTACA
GGCTACGTGCACATCTACGACATGGAGAAAGACTGCTGGGAAGAGGGACCCCAGCTGAACAATTCCATCT
CAGGCCTGGCAGCCTGTGTGCTCACCCTGCCTCGATCCCTGCTCCATGAGCAACCCCGAGGGACCCCCAA
CCGTAGCCAGGCAGATGCAGACTTTGCTTCTGAGGTGATGAGTGTGTCTGACTGGGAAGAGTTTGACAAC
TCTAGCGAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209634 protein sequence
Red=Cloning site Green=Tags(s)

MAEEQDFAQLCRLPTQPSHSHCVNNTYRSTQHSQALLRGLLALRDSGILFDVVLVVEGKHIEAHRILLAA
SCDYFRGMFAGGLKEMEQEEVLIHGVSYNAMCQILHFIYTSELELSLSNVQETLVAACQLQIPEIIHFCC
DFLMSWVDEENILDVYRLADLFDLNHLTQQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCE
TEVYEGALLYHYSLEQVQADQISLNEPPKLLETVRFPLMEAEVLQRLHDKLGPSPLRDTVASALMYHRNE
ILQPSLQGPQTELRSDFQCVVGFGGIHSTPSTILSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNF
VYLIGGDNNVQGFRAESRCWRYDPRHNRWFQIQSLQQEHADLCVCVVGKYIYAVAGRDYHNDLSAVERYD
PATNSWDYVAPLKKEVYAHAGTTLQGKMYITCGRRGEDYLKETHCYDPGSNTWHTLADGPVRRAWHGMAA
LLDKLFVIGGSNNDAGYRRDVHQVACYSCTSRQWSSVCPLPAGHGEPGIAVLDSRIYVLGGRSHNRGSRT
GYVHIYDMEKDCWEEGPQLNNSISGLAACVLTLPRSLLHEQPRGTPNRSQADADFASEVMSVSDWEEFDN
SSED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145479
ORF Size 1902 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145479.4, NP_663454.3
RefSeq Size 2633 bp
RefSeq ORF 1905 bp
Locus ID 224023
UniProt ID Q99JN2
Cytogenetics 16 11.01 cM
MW 71.7 kDa
Summary Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (By similarity). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).UniProtKB/Swiss-Prot Function
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"NM_145479" in other vectors (2)
SKU Description Size Price
MR209634L3 Lenti ORF clone of Klhl22 (Myc-DDK-tagged) - Mouse kelch-like 22 (Drosophila) (Klhl22) 10 ug
$891.00
MR209634L4 Lenti ORF clone of Klhl22 (mGFP-tagged) - Mouse kelch-like 22 (Drosophila) (Klhl22) 10 ug
$891.00

Plasmid Map for MR209634

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file-download TXT (FASTA format)

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Cloning scheme for MR209634
Circular map for MR209634

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