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Pls3 (NM_145629) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209599
Pls3 (Myc-DDK-tagged) - Mouse plastin 3 (T-isoform) (Pls3), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $587.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pls3
Synonyms AI115446; AL024105
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209599 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGAGATGGCGACCACCCAGATTTCCAAAGATGAGCTTGATGAACTCAAAGAAGCCTTTGCAAAAG
TTGATCTCAACAGCAATGGATTCATTTGCGACTATGAGCTTCATGAGCTTTTCAAGGAAGCTAATATGCC
ACTGCCCGGATATAAAGTGAGAGAAATTATTCAGAAACTCATGGTGGATGGTGACAGAAATAAAGATGGG
AAGATCAGCTTTAATGAGTTTGTTTATATTTTCCAAGAGGTAAAGAGCAGTGATATCGCTAAAACCTTCC
GAAAGGCCATCAACAGGAAAGAAGGGATTTGTGCTCTGGGAGGGACATCAGAGTTGTCCAGTGAAGGGAC
CCAGCATTCCTATTCAGAGGAAGAAAAATATGCTTTCGTTAACTGGATAAACAAAGCTTTGGAAAATGAT
CCCGATTGTAGACATGTCATACCCATGAACCCAAATACTGATGACCTGTTCAAAGCAGTTGGTGATGGGA
TCGTGCTCTGCAAAATGATCAACCTTTCAGTTCCTGATACAATTGACGAGAGAGCAATCAATAAGAAGAA
GCTTACACCCTTCATCATTCAGGAAAACTTGAACTTGGCCCTGAACTCTGCTTCTGCCATTGGGTGTCAC
GTTGTGAACATTGGTGCCGAAGATCTGCGGGCCGGGAAACCTCACCTGGTTTTGGGACTGCTCTGGCAGA
TTATTAAGATTGGCCTGTTTGCTGACATTGAATTAAGCAGGAATGAAGCACTGGCAGCTTTGCTTCGAGA
TGGTGAGACACTGGAGGAGCTTATGAAATTGTCTCCAGAAGAGCTTCTGCTGAGATGGGCCAACTTCCAT
CTGGAAAACTCAGGCTGGCAAAAGATCAACAACTTCAGCGCTGACATCAAGGATTCCAAAGCCTATTTCC
ATCTGCTCAATCAAATCGCACCGAAGGGACAAAAAGAAGGGGAGCCACGGATAGATATTAACATGTCAGG
TTTCAATGAAACTGATGATTTAAAGAGAGCCGAAAGTATGCTTCAACAAGCAGATAAATTAGGTTGCAGA
CAGTTCGTTACTCCAGCTGATGTTGTCAGCGGAAACCCCAAACTGAACTTGGCCTTTGTAGCTAACCTCT
TCAATAAATATCCAGCACTAACTAAACCTGAAAACCAGGATATTGACTGGACTCTTCTAGAAGGGGAAAC
TCGGGAAGAAAGAACCTTCCGCAACTGGATGAACTCTCTTGGTGTTAATCCCCACGTCAACCATCTCTAT
GCTGACCTGCAGGATGCTCTGGTGATCCTACAGCTGTATGAGAGGATTAAAGTTCCTGTGGACTGGAGTA
AAGTTAACAAGCCTCCATACCCAAAGCTGGGGGCCAACATGAAGAAGTTAGAAAACTGCAACTATGCAGT
TGAATTAGGGAAGAATCCAGCTAAATTCTCCTTGGTTGGCATTGGAGGACAAGACCTGAACGATGGCAAC
CCTACCTTGACCTTGGCTGTAGTCTGGCAGCTGATGAGAAGATATACCCTTAATGTTCTGGAAGATCTTG
GAGAGGGTCAGAAAGCAAATGATGACATCATTGTAAACTGGGTCAACAGAACATTGAGTGAAGCTGGAAA
GTCAACTTCCATTCAGAGTTTTAAGGACAAGACCATCAGCTCCAGTTTGGCGGTTGTGGATTTAATTGAC
GCCATCCAACCAGGCTGCATTAACTATGACCTTGTGAAGAGTGGGAATCTAACAGAAGATGACAAGCACA
ATAACGCCAAGTACGCAGTATCGATGGCTAGAAGAATCGGAGCCAGAGTATATGCCCTCCCTGAAGACCT
TGTGGAAGTAAAACCGAAGATGGTCATGACTGTGTTTGCCTGTTTGATGGGCAGAGGGATGAAGAGAGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209599 protein sequence
Red=Cloning site Green=Tags(s)

MDEMATTQISKDELDELKEAFAKVDLNSNGFICDYELHELFKEANMPLPGYKVREIIQKLMVDGDRNKDG
KISFNEFVYIFQEVKSSDIAKTFRKAINRKEGICALGGTSELSSEGTQHSYSEEEKYAFVNWINKALEND
PDCRHVIPMNPNTDDLFKAVGDGIVLCKMINLSVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCH
VVNIGAEDLRAGKPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEELLLRWANFH
LENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRIDINMSGFNETDDLKRAESMLQQADKLGCR
QFVTPADVVSGNPKLNLAFVANLFNKYPALTKPENQDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLY
ADLQDALVILQLYERIKVPVDWSKVNKPPYPKLGANMKKLENCNYAVELGKNPAKFSLVGIGGQDLNDGN
PTLTLAVVWQLMRRYTLNVLEDLGEGQKANDDIIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLID
AIQPGCINYDLVKSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMGRGMKRV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145629
ORF Size 1890 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145629.1, NP_663604.1
RefSeq Size 3251 bp
RefSeq ORF 1893 bp
Locus ID 102866
UniProt ID Q99K51
Cytogenetics X A7.3
MW 70.7 kDa
Summary Actin-bundling protein.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145629" in other vectors (4)
SKU Description Size Price
MC219894 Pls3 (untagged) - Mouse plastin 3 (T-isoform) (Pls3), transcript variant 1, (10ug) 10 ug
$646.00
MG209599 Pls3 (tGFP-tagged) - Mouse plastin 3 (T-isoform) (Pls3) 10 ug
$787.00
MR209599L3 Lenti ORF clone of Pls3 (Myc-DDK-tagged) - Mouse plastin 3 (T-isoform) (Pls3), transcript variant 1 10 ug
$887.00
MR209599L4 Lenti ORF clone of Pls3 (mGFP-tagged) - Mouse plastin 3 (T-isoform) (Pls3), transcript variant 1 10 ug
$887.00

Plasmid Map for MR209599

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209599
Circular map for MR209599

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