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Il27ra (NM_016671) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209519
Il27ra (Myc-DDK-tagged) - Mouse interleukin 27 receptor, alpha (Il27ra)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $581.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Il27ra
Synonyms CRL1; IL-27R; Tccr; WSX-1; Wsx1; zcytor1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209519 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGGCTCCGGGTTGCACGCCTCACGCCGTTGGAGCTTCTGCTGTCGCTGATGTCGCTGCTGCTCG
GGACGCGGCCCCACGGCAGTCCAGGCCCACTGCAGTGCTACAGCGTCGGTCCCCTGGGAATCCTGAACTG
CTCCTGGGAACCTTTGGGCGACCTGGAGACTCCACCTGTGCTGTATCACCAGAGTCAGAAATACCATCCC
AATAGAGTCTGGGAGGTGAAGGTGCCTTCCAAACAGAGTTGGGTGACCATTCCCCGGGAACAGTTCACCA
TGGCTGACAAACTCCTCATCTGGGGGACACAAAAGGGACGGCCTCTGTGGTCCTCTGTCTCTGTGAACCT
GGAGACCCAAATGAAGCCAGACACACCTCAGATCTTCTCTCAAGTGGATATTTCTGAGGAAGCAACCCTG
GAGGCCACTGTGCAGTGGGCGCCGCCCGTGTGGCCACCGCAGAAAGCTCTCACCTGTCAGTTCCGGTACA
AGGAATGCCAGGCTGAAGCATGGACCCGGCTGGAGCCCCAGCTGAAGACAGATGGGCTGACTCCTGTTGA
GATGCAGAACCTGGAACCTGGCACCTGCTACCAGGTGTCTGGCCGCTGCCAGGTGGAGAACGGATATCCA
TGGGGCGAGTGGAGTTCGCCCCTGTCCTTCCAGACGCCATTCTTAGATCCTGAAGATGTGTGGGTATCGG
GGACCGTCTGTGAAACTTCTGGCAAACGGGCAGCCCTGCTTGTCTGGAAGGACCCAAGACCTTGTGTGCA
GGTGACTTACACAGTCTGGTTTGGGGCTGGAGATATTACTACAACTCAAGAAGAGGTCCCGTGCTGCAAG
TCCCCTGTCCCTGCATGGATGGAGTGGGCTGTGGTCTCTCCTGGCAACAGCACCAGCTGGGTGCCTCCCA
CCAACCTGTCTCTGGTGTGCTTGGCTCCAGAATCTGCCCCCTGTGACGTGGGAGTGAGCAGTGCTGATGG
GAGCCCAGGGATAAAGGTGACCTGGAAACAAGGGACCAGGAAACCATTGGAGTATGTGGTGGACTGGGCT
CAAGATGGTGACAGCCTGGACAAGCTCAACTGGACCCGTCTCCCCCCTGGAAACCTCAGCACATTGTTAC
CAGGGGAGTTCAAAGGAGGGGTCCCCTATCGAATTACAGTGACTGCAGTATACTCTGGAGGATTAGCTGC
TGCACCCTCAGTTTGGGGATTCAGAGAGGAGTTAGTACCCCTTGCTGGGCCAGCAGTTTGGCGACTTCCA
GATGACCCCCCAGGGACACCTGTTGTAGCCTGGGGAGAAGTACCAAGACACCAGCTCAGAGGCCAGGCTA
CTCACTACACCTTCTGCATACAGAGCAGAGGCCTCTCCACTGTCTGCAGGAACGTGAGCAGTCAAACCCA
GACTGCCACTCTGCCCAACCTTCACTCGGGTTCCTTCAAGCTGTGGGTGACGGTGTCCACCGTTGCAGGA
CAGGGCCCACCTGGTCCCGACCTTTCACTTCACCTACCAGATAATAGGATCAGGTGGAAAGCTCTGCCCT
GGTTTCTGTCCCTGTGGGGTTTGCTTCTGATGGGCTGTGGCCTGAGCCTGGCCAGTACCAGGTGCCTACA
GGCCAGGTGCTTACACTGGCGACACAAGTTGCTTCCCCAGTGGATCTGGGAGAGGGTTCCTGATCCTGCC
AACAGCAATTCTGGGCAACCTTACATCAAGGAGGTGAGCCTGCCCCAACCGCCCAAGGACGGACCCATCC
TGGAGGTGGAGGAAGTGGAGCTACAGCCTGTTGTGGAGTCCCCTAAAGCCTCTGCCCCGATTTACTCTGG
GTATGAGAAACACTTCCTGCCCACACCAGAGGAGCTGGGCCTTCTAGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209519 protein sequence
Red=Cloning site Green=Tags(s)

MNRLRVARLTPLELLLSLMSLLLGTRPHGSPGPLQCYSVGPLGILNCSWEPLGDLETPPVLYHQSQKYHP
NRVWEVKVPSKQSWVTIPREQFTMADKLLIWGTQKGRPLWSSVSVNLETQMKPDTPQIFSQVDISEEATL
EATVQWAPPVWPPQKALTCQFRYKECQAEAWTRLEPQLKTDGLTPVEMQNLEPGTCYQVSGRCQVENGYP
WGEWSSPLSFQTPFLDPEDVWVSGTVCETSGKRAALLVWKDPRPCVQVTYTVWFGAGDITTTQEEVPCCK
SPVPAWMEWAVVSPGNSTSWVPPTNLSLVCLAPESAPCDVGVSSADGSPGIKVTWKQGTRKPLEYVVDWA
QDGDSLDKLNWTRLPPGNLSTLLPGEFKGGVPYRITVTAVYSGGLAAAPSVWGFREELVPLAGPAVWRLP
DDPPGTPVVAWGEVPRHQLRGQATHYTFCIQSRGLSTVCRNVSSQTQTATLPNLHSGSFKLWVTVSTVAG
QGPPGPDLSLHLPDNRIRWKALPWFLSLWGLLLMGCGLSLASTRCLQARCLHWRHKLLPQWIWERVPDPA
NSNSGQPYIKEVSLPQPPKDGPILEVEEVELQPVVESPKASAPIYSGYEKHFLPTPEELGLLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016671
ORF Size 1869 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016671.2, NP_057880.1
RefSeq Size 2671 bp
RefSeq ORF 1872 bp
Locus ID 50931
UniProt ID O70394
Cytogenetics 8 C2
MW 69 kDa
Summary Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.UniProtKB/Swiss-Prot Function
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"NM_016671" in other vectors (3)
SKU Description Size Price
MG209519 Il27ra (tGFP-tagged) - Mouse interleukin 27 receptor, alpha (Il27ra) 10 ug
$489.00 $781.00
MR209519L3 Lenti ORF clone of Il27ra (Myc-DDK-tagged) - Mouse interleukin 27 receptor, alpha (Il27ra) 10 ug
$881.00
MR209519L4 Lenti ORF clone of Il27ra (mGFP-tagged) - Mouse interleukin 27 receptor, alpha (Il27ra) 10 ug
$881.00

Plasmid Map for MR209519

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR209519
Circular map for MR209519

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