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Frmd6 (NM_028127) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209510
Frmd6 (Myc-DDK-tagged) - Mouse FERM domain containing 6 (Frmd6)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $580.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Frmd6
Synonyms 2610019M19Rik; 4930488L10Rik; AW212977
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209510 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAACTGACCTTCCATAACAACAAAGCCATGCAGGACCGTCGCAGAGTGTGTATTTTCCTCCCCA
ATGACAAGTCCGTGAGCATCATCATAAATGTTAAAATTCTGTGTCACCAGTTGCTGGTCCAGGTGTGTGA
CCTGCTCAGGTTAAAGGACAGTCACCTCTTTGGTCTCAGTGTTATACAAAATAATGAACATGTATATATG
GAATTGTCACAAAAGCTTTATAAGTATTGTCCAAAAGAATGGAAAAAGGAGGCCAGCAAGGTACGGCAAT
ACGAAGTCACTTGGGGCATCGACCAGTTTGGGCCCCCCATGATCATCCACTTCCGGGTGCAGTACTACGT
GGAGAATGGGAAGCTGATCAGTGACCGAATTGCAAGATACTATTATTACTGGCACCTACGGAAACAGGTG
CTGCACTCCCAGTGTGTGCTCAGAGAGGAGGCCTACTTCCTGCTGGCAGCCTTTGCACTGCAAGCTGACC
TCGGCAACTTCAAAAGGAAACTGCACCACGGAGACTACTTTGAGCCAGAGGCTTACTTCCCGGCATGGGT
TGTTTCCAAGCGGGGGAAGGACTACATCCTGAAACACATCCCAAACATGCACAAGGACCAGTTTGCCCTG
ACGGCCTCCGAGGCCTACCTAAAGTACATCAAAGAAGCCGTCCGACTGGACGACGTCGCCATCCATTACT
ACAGACTGTACAAGGATAAAAGGGAGGCTGAAGGCTCACTGACCCTAGGACTGACCATGCGAGGAATACA
GATTTTTCAGAATCTAGAAGAAGAGAAACAATTGCTCTATGATTTCCCCTGGACAAATGTTGGGAAGTTG
GTGTTTGTGGGCAAGAAGTTTGAGATTTTGCCAGATGGCCTTCCCTCCGCCAGGAAGCTGGTCTACTACA
CAGGGTGTCCCACGCGCTCCCGGCATCTCCTGCAGCTCCTGAGCAACAGCCACCGCCTCTACATGAACCT
GCAGCCCGTCCTGCGCCACCTCCGCAAGCAGGAGGAGAATGAAGAGAAAAAGCAGTACCGGGAATCCTAC
ATCAGCGACAACCTGGACCTTGACATGGACCCGCTGGAAAAGCGGTCCCGAGCCAGTGGGAGCAGCGCTG
GCAGCGTGAAGCATAAGCGCCTGTCCCGCCACTCCACGGCCAGCCACAGCAGCTCCCACACCTCCGGCAT
CGAGGCAGACACCAAGCCCCGGGACCCAGGGCCGGAAGACAGCTGTTCAGGCAGCGCCATGCACCGGAAG
CTGAAGACCTGCAGCTCCATGACCAGCCACGGCAGCTCCCACACCTCTGGGGTTGAGAGTGGAGGCAAAG
ACCGCCTGGAAGAGGACTCGCAAGATGAGGAAATCGAGATGCTGGTGGATGACCCCAGGGACCTGGAGCC
GATGCCTGAAGAGTCGCTGGAAGTCAGCCCAGAGATGTGTATCTACATCACGGAAGATATGCTCCTGTCG
AGGAAGCTGAACGGACACTCAGGGTTAATTGTGAAAGAAATCGGCTCCTCCACCTCCAGCTCTTCGGAAA
CGGTTGTCAGGCTGCGTGGACAGAGCACCGACTCCCTTCCACAGACGATATGTCGAAAACCAAAGACTTC
CACCGATCGCCATAGCCTGAGCCTTGACGACATCAGACTGTACCAGAAAGACTTCCTGCGCATCGCGGGC
CTGTGTCAGGACACTGCTCAGAGCTACACGTTTGGGTGTGGCCATGAACTGGATGAGAGCGGTCTCTACT
GCAACAGCTGCCTGGCTCAGCAGTGTGTCAACATACAGGACGCATTCCCAGTGAAAAGAGCCAGCAAGTA
CTTTTCTCTGGACCTTACTCACGACGAAGTCCCAGAGTTCGTCGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209510 protein sequence
Red=Cloning site Green=Tags(s)

MNKLTFHNNKAMQDRRRVCIFLPNDKSVSIIINVKILCHQLLVQVCDLLRLKDSHLFGLSVIQNNEHVYM
ELSQKLYKYCPKEWKKEASKVRQYEVTWGIDQFGPPMIIHFRVQYYVENGKLISDRIARYYYYWHLRKQV
LHSQCVLREEAYFLLAAFALQADLGNFKRKLHHGDYFEPEAYFPAWVVSKRGKDYILKHIPNMHKDQFAL
TASEAYLKYIKEAVRLDDVAIHYYRLYKDKREAEGSLTLGLTMRGIQIFQNLEEEKQLLYDFPWTNVGKL
VFVGKKFEILPDGLPSARKLVYYTGCPTRSRHLLQLLSNSHRLYMNLQPVLRHLRKQEENEEKKQYRESY
ISDNLDLDMDPLEKRSRASGSSAGSVKHKRLSRHSTASHSSSHTSGIEADTKPRDPGPEDSCSGSAMHRK
LKTCSSMTSHGSSHTSGVESGGKDRLEEDSQDEEIEMLVDDPRDLEPMPEESLEVSPEMCIYITEDMLLS
RKLNGHSGLIVKEIGSSTSSSSETVVRLRGQSTDSLPQTICRKPKTSTDRHSLSLDDIRLYQKDFLRIAG
LCQDTAQSYTFGCGHELDESGLYCNSCLAQQCVNIQDAFPVKRASKYFSLDLTHDEVPEFVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028127
ORF Size 1866 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028127.3, NP_082403.2
RefSeq Size 4695 bp
RefSeq ORF 1869 bp
Locus ID 319710
UniProt ID Q8C0V9
Cytogenetics 12 C2
MW 71.7 kDa
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Documents
Resources
"NM_028127" in other vectors (2)
SKU Description Size Price
MR209510L3 Lenti ORF clone of Frmd6 (Myc-DDK-tagged) - Mouse FERM domain containing 6 (Frmd6) 10 ug
$880.00
MR209510L4 Lenti ORF clone of Frmd6 (mGFP-tagged) - Mouse FERM domain containing 6 (Frmd6) 10 ug
$880.00

Plasmid Map for MR209510

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Cloning scheme for MR209510
Circular map for MR209510

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