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Slc6a3 (NM_010020) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209484
Slc6a3 (Myc-DDK-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $577.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc6a3
Synonyms DAT; Dat1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209484 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTAAAAGCAAATGCTCCGTGGGACCAATGTCTTCTGTGGTGGCCCCGGCTAAAGAGCCCAATGCTG
TGGGCCCCAGAGAGGTGGAGCTCATCTTGGTCAAGGAGCAGAATGGAGTGCAGCTGACCAACTCCACCCT
CATCAACCCACCGCAGACACCAGTGGAGGTTCAAGAGCGGGAGACCTGGAGCAAGAAAATCGATTTCCTG
CTCTCAGTCATCGGCTTCGCTGTGGACCTGGCCAATGTCTGGAGGTTTCCCTACCTGTGCTACAAAAATG
GTGGAGGTGCCTTCCTGGTGCCCTACCTGCTCTTCATGGTTATTGCCGGGATGCCCCTCTTCTACATGGA
GCTGGCTCTCGGGCAGTTCAACAGAGAAGGAGCTGCTGGTGTCTGGAAGATCTGCCCTGTCCTGAAAGGT
GTGGGCTTCACTGTCATCCTCATCTCTTTCTACGTGGGCTTCTTCTACAATGTCATCATTGCATGGGCAC
TGCACTACTTCTTCTCCTCCTTCACCATGGACCTCCCATGGATCCACTGCAACAACACCTGGAACAGCCC
CAACTGCTCTGATGCACATAGCAGCAACTCTAGCGATGGCCTGGGCCTCAACGACACCTTTGGGACCACA
CCCGCTGCTGAGTATTTTGAGCGTGGTGTGCTGCACCTCCATCAGAGTCGTGGCATTGATGACCTGGGCC
CTCCACGGTGGCAGCTCACAGCCTGCCTGGTGCTGGTCATTGTTCTGCTCTACTTCAGCCTGTGGAAGGG
AGTAAAGACTTCAGGGAAGGTGGTGTGGATCACAGCTACCATGCCCTATGTAGTCCTCACAGCCCTGCTC
CTGCGTGGAGTCACCCTCCCTGGGGCCATGGATGGCATCAGAGCATACCTCAGTGTGGACTTCTACCGTC
TCTGTGAGGCATCTGTGTGGATCGATGCCGCCACCCAGGTGTGCTTCTCCCTTGGCGTTGGGTTTGGGGT
GCTGATTGCCTTCTCCAGTTACAATAAGTTCACCAATAACTGCTATAGAGATGCAATCATCACCACCTCC
ATTAACTCCCTGACGAGCTTCTCCTCTGGCTTCGTTGTCTTCTCCTTCCTGGGGTACATGGCACAGAAGC
ACAATGTGCCCATCAGGGATGTGGCCACAGATGGACCTGGGTTGATCTTCATCATCTACCCTGAGGCAAT
CGCCACACTCCCGCTGTCTTCAGCCTGGGCCGCTGTCTTCTTCCTCATGCTGCTCACTCTGGGTATCGAC
AGTGCCATGGGGGGCATGGAGTCTGTGATCACTGGGCTTGTCGATGAGTTCCAGCTGCTACATCGGCATC
GAGAACTCTTCACTCTTGGCATTGTCCTGGCTACTTTCCTGCTGTCTCTCTTCTGTGTCACCAACGGTGG
CATCTATGTCTTCACACTGCTGGACCACTTTGCAGCTGGCACATCTATCCTCTTTGGAGTGCTCATTGAA
GCCATTGGGGTGGCCTGGTTCTACGGTGTCCAGCAATTCAGTGATGACATCAAGCAGATGACTGGGCAGC
GACCCAACCTGTACTGGCGGCTATGCTGGAAGCTGGTCAGCCCCTGCTTCCTTCTGTATGTGGTCGTGGT
CAGCATTGTGACCTTCAGACCCCCACACTATGGAGCCTACATCTTCCCAGACTGGGCCAATGCCCTGGGC
TGGATCATTGCCACATCCTCCATGGCCATGGTGCCCATTTATGCCACCTATAAGTTCTGCAGCCTGCCAG
GGTCCTTCCGAGAGAAACTGGCCTATGCCATCACACCTGAGAAAGACCGCCAGCTAGTGGACAGAGGGGA
GGTGCGCCAATTCACGCTGCGCCATTGGCTGTTGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209484 protein sequence
Red=Cloning site Green=Tags(s)

MSKSKCSVGPMSSVVAPAKEPNAVGPREVELILVKEQNGVQLTNSTLINPPQTPVEVQERETWSKKIDFL
LSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPVLKG
VGFTVILISFYVGFFYNVIIAWALHYFFSSFTMDLPWIHCNNTWNSPNCSDAHSSNSSDGLGLNDTFGTT
PAAEYFERGVLHLHQSRGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALL
LRGVTLPGAMDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIITTS
INSLTSFSSGFVVFSFLGYMAQKHNVPIRDVATDGPGLIFIIYPEAIATLPLSSAWAAVFFLMLLTLGID
SAMGGMESVITGLVDEFQLLHRHRELFTLGIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIE
AIGVAWFYGVQQFSDDIKQMTGQRPNLYWRLCWKLVSPCFLLYVVVVSIVTFRPPHYGAYIFPDWANALG
WIIATSSMAMVPIYATYKFCSLPGSFREKLAYAITPEKDRQLVDRGEVRQFTLRHWLLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010020
ORF Size 1857 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010020.2
RefSeq Size 3456 bp
RefSeq ORF 1860 bp
Locus ID 13162
UniProt ID Q61327
Cytogenetics 13 40.1 cM
MW 68.8 kDa
Summary Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010020" in other vectors (4)
SKU Description Size Price
MC219693 Slc6a3 (untagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), (10ug) 10 ug
$635.00
MG209484 Slc6a3 (tGFP-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3) 10 ug
$777.00
MR209484L3 Lenti ORF clone of Slc6a3 (Myc-DDK-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3) 10 ug
$877.00
MR209484L4 Lenti ORF clone of Slc6a3 (mGFP-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3) 10 ug
$877.00

Plasmid Map for MR209484

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209484
Circular map for MR209484

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