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0610010F05Rik (NM_027860) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209438
0610010F05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610010F05 gene (0610010F05Rik)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $573.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 0610010F05Rik
Synonyms Kiaa1841; mKIAA1841
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209438 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCGTGGATATCCAGAAAACAATAATTTTCTGAATAATAATAACCAAATGGTATTGGATATGATTC
TTTATCCATTGATTGGAATCCCTCAGACCATCAACTGGGAAACTGTGGCAAGGCTTGTGCCTGGATTAAC
GCCCAAAGAGTGTGTGAAAAGGTTTGATGAGCTAAAGAGCTGTGGAAGTTCACCTGTTGACAACCAGTAT
AACCCTTTAATGGCTACTGGAGAGGGTCCTGTTGAAACTTTAGCCACTTACATTAAATCTTCACTTCTGG
ACACACAAGGAGATTTTCAAGAGACTCCTGTTGATCAAGATACAGTTTCTAAAGCAGGAAGACATAGTAT
AGCTACCACAAGGAATTGCTCTTCAGAAAGTGAAAACTGTACTGCTCGTAATGCTGGAGAAGAGACTGGA
GAATCTGAAGGGCCAAACATGGTGATCCATGTATGTGATGAAGCAAAAAGCTTGAAAGAAGATTTTATTT
GCCCACGAGATCTTTTGATATCAGAAATGAAGTACTTTGCTGAATATTTATCCATGGATGCGCAGCGCTG
GGAAGAAGTGGACATTTCAGTTCATTGCGATGTTCACATTTTCAACTGGCTGATAAAATATGTTAAACGG
AACACTAAGGAGAGTAAAGATTGTGAGATTCCCGCTTTAGAGCCAGGGAATGTCATTTCAATCCTTATTT
CATCAGAGTTCTTGAAGATGGATTCACTAGTTGAACAATGTATTCAATATTGCCATAAAAATATGAATGC
CATAGTAGCTGCTCCATGCAACATGAACTGTATCAATGCAAACCTTCTTACACGCATAGCTGATCTGTTC
ACACACAATGAAATTGATGACTTAAAAGACAAAAAAGATAAGTTTAGGAGTAAACTGTTTTGTAAGAAGA
TTGAAAGACTCTTTGATCCTGAATACTCCAATCCAGATTCTAGAAACAATGCAGCAACACTCTATCGATG
CTGTTTGTGTAAGAAACTTTTAACCAGAGAAACAGAAAGAAGAATTCCTTGCATCCCTGGAAAAATCAAT
GTGGACCGACATGGAAATATTATCTACATCCACATAAGAGATAAAACCTGGGATGTACATGAATATTTGA
ATAGTCTTTTTGAAGAATTAAAATCTTGGAGAGATGTTTATTGGCGTTTGTGGGGCACAGTCAACTGGCT
GACTTGTTCAAGGTGTTACCAGGCTTTTCTCTGTATTGAGTTCTCACATTGTCAGTATCACTCAGAAGTG
GTAGTTTATTCTAGCACAGTAAATTCACTAAACACTGTGGGCACTGGAATTTATCCCTGCTGTAACCAGA
AGGTTCTTCGATTTGATCCTACTCAGCTCACAAAGGGCTGTAAGGTGAGGGACCACATGGTGGTTCTTCA
TGATCAAGGAGAAAATGATGATTCTCCTTCTTGCCCACCTGCTAAAATACTGGATGATCTGCACAAGCAC
AAAGACGTCATTGCTGTGCCTTTTTTAAAAGATGCAGTTAGTGATCCTGGGGTTGGCTCCTGTGATGAAA
AAGGCCTGGAATATGAAATTTTACTGGAACCAAATACACCGTGGGGCTCCAAAACTGGGGAGCTCAATGC
TTTCTTGTCACTGAAAAACTGGACTCTGCAGTTGAAACAACAGTCGCTGTTCTCAGAAGAGGAAGAGTAT
ACCACGGGGTCTGAGGTCACTGAAGATGAAGTCGGTGATGAAGAAGAAATTGCCAAGAAACAAAGGAAAA
AGGAGAAGCCAAAGAAATTTACTAAGCCGCCAAAAAAAACAACTGTCCTCACCCTGTTCTCAAAAGAAAG
AGAAGACACTGGAGAAGTCAACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209438 protein sequence
Red=Cloning site Green=Tags(s)

MSRGYPENNNFLNNNNQMVLDMILYPLIGIPQTINWETVARLVPGLTPKECVKRFDELKSCGSSPVDNQY
NPLMATGEGPVETLATYIKSSLLDTQGDFQETPVDQDTVSKAGRHSIATTRNCSSESENCTARNAGEETG
ESEGPNMVIHVCDEAKSLKEDFICPRDLLISEMKYFAEYLSMDAQRWEEVDISVHCDVHIFNWLIKYVKR
NTKESKDCEIPALEPGNVISILISSEFLKMDSLVEQCIQYCHKNMNAIVAAPCNMNCINANLLTRIADLF
THNEIDDLKDKKDKFRSKLFCKKIERLFDPEYSNPDSRNNAATLYRCCLCKKLLTRETERRIPCIPGKIN
VDRHGNIIYIHIRDKTWDVHEYLNSLFEELKSWRDVYWRLWGTVNWLTCSRCYQAFLCIEFSHCQYHSEV
VVYSSTVNSLNTVGTGIYPCCNQKVLRFDPTQLTKGCKVRDHMVVLHDQGENDDSPSCPPAKILDDLHKH
KDVIAVPFLKDAVSDPGVGSCDEKGLEYEILLEPNTPWGSKTGELNAFLSLKNWTLQLKQQSLFSEEEEY
TTGSEVTEDEVGDEEEIAKKQRKKEKPKKFTKPPKKTTVLTLFSKEREDTGEVNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027860
ORF Size 1845 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027860.1
RefSeq Size 4140 bp
RefSeq ORF 2157 bp
Locus ID 71675
UniProt ID Q68FF0
Cytogenetics 11 A3.2
MW 70.3 kDa
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Documents
Resources
"NM_027860" in other vectors (3)
SKU Description Size Price
MC205216 0610010F05Rik (untagged) - Mouse RIKEN cDNA 0610010F05 gene (0610010F05Rik), (10ug) 10 ug
$658.00
MR209438L3 Lenti ORF clone of 0610010F05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610010F05 gene (0610010F05Rik) 10 ug
$873.00
MR209438L4 Lenti ORF clone of 0610010F05Rik (mGFP-tagged) - Mouse RIKEN cDNA 0610010F05 gene (0610010F05Rik) 10 ug
$873.00

Plasmid Map for MR209438

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209438
Circular map for MR209438

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