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Igsf8 (NM_080419) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209404
Igsf8 (Myc-DDK-tagged) - Mouse immunoglobulin superfamily, member 8 (Igsf8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $570.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Igsf8
Synonyms ESTM34; EWI-2; KCT-4; PGRL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209404 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGTCCCTAGCCCCACGCCGCTGAGTTCGCTCCTGCTGCTGCTGCTAATACTTGGAACCAGGTGCT
ACGCCCGGCAGGTGCATGTCCCCAGGGGACCTCTTTACCGGGTGGCTGGCACCGCTGTCTCTATCTCCTG
CAACGTGAGTGACTATGAGGGCCCTGCCCAGCAAGACTTCGAGTGGTTCATGTACAGACCAGAGGCCCCA
GCTACGTCCCTGGGCATTGTCAGCACCAAGGATAGCCAGTTCTCCTATGCTGTCTTTGGGCCTCGTGTGG
CATCTGGTGACCTGCAGGTGCAGCGCCTGAAGGGAGATTCGGTGGTGCTCAAGATTGCTCGCCTGCAGGC
CCAGGACTCTGGCTTTTATGAGTGCTACACCCCCTCCACAGATACGCAGTACCTGGGCAACTACAGTGCC
AAGGTGGAGCTGAGAGTTCTTCCAGATGAGCTGCAGGTATCTGCTGCCCCTCCAGGGCCCCGAGGGCGCC
AGGCTGCCACCTCCCCCTCACGCCTCACAGTGCACGAGGGGCAGGAGCTTGCGCTGGGCTGCCTGGCTCA
GACCAAAACAAAGAAACACACACACCTGTCGGTGTCCTTTGGGAGAGCCATTCCGGAGGCGCCAGTGGGG
CGAGCCACTCTACAGGAAGTAGTGGGACTGCACTCCGACATGGCGGTGGAGGCTGGAGCGCCCTACGCTG
AGAGGCTAGCTTCTGGGGAGCTTCGGCTGAGCAAGGAAGGGACTGATCGGTACCGAATGGTGGTTGGGGG
TGCCCAGGCTGGAGACTCGGGCACCTACCACTGTACGGCCGCTGAGTGGATTCAGGATCCTGACGGCTCC
TGGGTCCAGGTCGCAGAGAAGAGGGCAGTCCTGGCTCATGTTGATGTGCAGACTCTGTCCAGCCAGCTGG
CGGTGACCGTGGGACCTGGTGAACGTCGGATTGGCCCAGGCGAACCCTTAGAACTGCTGTGCAATGTGTC
CGGTGCACTGCCCCCACCAGGCCGTCATGCTGCGTACTCTGTGGGCTGGGAGATGGCCCCTGCAGGGGCT
CCTGGACCCGGCCGCCTGGTGGCCCAGCTGGACACGGAAGGTATAGGCAGCCTGGGCCCTGGCTATGAGG
ACCGGCACATTGCCATGGAGAAAGTAGCATCCAGAACCTACCGACTGCGGCTGGAGGCTGCCAGGCCCGC
TGATGCAGGCACCTACCGCTGCCTCGCCAAAGCCTATGTTCGAGGGTCTGGGACCCGGCTTCGTGAAGCG
GCCAGTGCTCGTTCCCGGCCTCTCCCTGTGCATGTGAGAGAAGAAGGCGTGGTGCTAGAGGCTGTGGCGT
GGCTAGCAGGGGGCACTGTGTACCGGGGAGAAACGGCCTCCCTGCTATGCAACATATCTGTGCGGGGCGG
CCCCCCAGGGCTGCGACTAGCAGCCAGCTGGTGGGTGGAGAGGCCAGAGGAGGGCGAGCTGAGCACTGGC
CCTGCTCAGCTTGTGGGTGGAGTGGGTCAGGACGGTGTGGCAGAGCTGGGAGTCCGGCCTGGAGGGGGTC
CTGTCAGTGTGGAGCTGGTGGGACCCAGAAGTCATCGGCTAAGACTGCACGGCTTGGGGCCTGAGGATGA
AGGCATATACCACTGTGCCCCAAGTGCCTGGGTGCAGCATGCGGACTACAGCTGGTACCAGGCAGGCAGT
GCGCGCTCCGGGCCTGTCACAGTCTACCCCTACACGCATGCTGTGGATACCCTATTTGTGCCCCTGTTGG
TGGGTACAGGAGTCGCCCTAGTTACCGGCGCCAGCGTCCTGGCTACCATCACCTGCTGCTTTATGAAGAG
GATGCGGAAGCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209404 protein sequence
Red=Cloning site Green=Tags(s)

MGVPSPTPLSSLLLLLLILGTRCYARQVHVPRGPLYRVAGTAVSISCNVSDYEGPAQQDFEWFMYRPEAP
ATSLGIVSTKDSQFSYAVFGPRVASGDLQVQRLKGDSVVLKIARLQAQDSGFYECYTPSTDTQYLGNYSA
KVELRVLPDELQVSAAPPGPRGRQAATSPSRLTVHEGQELALGCLAQTKTKKHTHLSVSFGRAIPEAPVG
RATLQEVVGLHSDMAVEAGAPYAERLASGELRLSKEGTDRYRMVVGGAQAGDSGTYHCTAAEWIQDPDGS
WVQVAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLELLCNVSGALPPPGRHAAYSVGWEMAPAGA
PGPGRLVAQLDTEGIGSLGPGYEDRHIAMEKVASRTYRLRLEAARPADAGTYRCLAKAYVRGSGTRLREA
ASARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLRLAASWWVERPEEGELSTG
PAQLVGGVGQDGVAELGVRPGGGPVSVELVGPRSHRLRLHGLGPEDEGIYHCAPSAWVQHADYSWYQAGS
ARSGPVTVYPYTHAVDTLFVPLLVGTGVALVTGASVLATITCCFMKRMRKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080419
ORF Size 1833 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080419.2
RefSeq Size 2256 bp
RefSeq ORF 1836 bp
Locus ID 140559
UniProt ID Q8R366
Cytogenetics 1 79.59 cM
MW 65 kDa
Summary May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.UniProtKB/Swiss-Prot Function
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"NM_080419" in other vectors (5)
SKU Description Size Price
MG209404 Igsf8 (tGFP-tagged) - Mouse immunoglobulin superfamily, member 8 (Igsf8) 10 ug
$489.00 $770.00
MR209404L1 Lenti ORF clone of Igsf8 (Myc-DDK-tagged) - Mouse immunoglobulin superfamily, member 8 (Igsf8) 10 ug
$870.00
MR209404L2 Lenti ORF clone of Igsf8 (mGFP-tagged) - Mouse immunoglobulin superfamily, member 8 (Igsf8) 10 ug
$870.00
MR209404L3 Lenti ORF clone of Igsf8 (Myc-DDK-tagged) - Mouse immunoglobulin superfamily, member 8 (Igsf8) 10 ug
$870.00
MR209404L4 Lenti ORF clone of Igsf8 (mGFP-tagged) - Mouse immunoglobulin superfamily, member 8 (Igsf8) 10 ug
$870.00

Plasmid Map for MR209404

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209404
Circular map for MR209404

file-alt Citations

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