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Afp (NM_007423) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209348
Afp (Myc-DDK-tagged) - Mouse alpha fetoprotein (Afp)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $564.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Afp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209348 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTGGATCACACCCGCTTCCCTCATCCTCCTGCTACATTTCGCTGCGTCCAAAGCATTGCACGAAA
ATGAGTTTGGGATAGCTTCCACGTTAGATTCCTCCCAGTGCGTGACGGAGAAGAATGTGCTTAGCATAGC
TACCATCACCTTTACCCAGTTTGTTCCGGAAGCCACCGAGGAGGAAGTGAACAAAATGACTAGCGATGTG
TTGGCTGCAATGAAGAAAAACTCTGGCGATGGGTGTTTAGAAAGCCAGCTATCTGTGTTTCTGGATGAAA
TTTGTCATGAGACGGAACTCTCTAACAAGTATGGACTCTCAGGCTGCTGCAGCCAAAGTGGAGTGGAAAG
ACATCAGTGTCTGCTGGCACGCAAGAAGACTGCTCCGGCCTCTGTCCCACCCTTCCAGTTTCCAGAACCT
GCCGAGAGTTGCAAAGCACATGAAGAAAACAGGGCAGTGTTCATGAACAGGTTCATCTATGAAGTGTCAA
GGAGGAACCCCTTCATGTATGCCCCAGCCATTCTGTCCTTGGCTGCTCAGTACGACAAGGTCGTTCTGGC
ATGCTGCAAAGCTGACAACAAGGAGGAGTGCTTCCAGACAAAGAGAGCATCCATTGCAAAGGAATTAAGA
GAAGGAAGCATGTTAAATGAGCATGTATGTTCAGTGATAAGAAAATTTGGATCCCGAAACCTCCAGGCAA
CAACCATTATTAAGCTAAGTCAAAAGTTAACTGAAGCAAATTTTACTGAGATTCAGAAGCTGGCCCTGGA
TGTGGCTCACATCCACGAGGAGTGTTGCCAAGGAAACTCGCTGGAGTGTCTGCAGGATGGGGAAAAAGTC
ATGACATATATATGTTCTCAACAAAATATTCTGTCAAGCAAAATAGCAGAGTGCTGCAAATTACCCATGA
TCCAACTAGGCTTCTGCATAATTCACGCAGAGAATGGCGTCAAACCTGAAGGCTTATCTCTAAATCCAAG
CCAGTTTTTGGGAGACAGAAATTTTGCCCAATTTTCTTCAGAGGAAAAAATCATGTTCATGGCAAGCTTT
CTTCATGAATACTCAAGAACTCACCCCAACCTTCCTGTCTCAGTCATTCTAAGAATTGCTAAAACGTACC
AGGAAATATTGGAGAAGTGTTCCCAGTCTGGAAATCTACCTGGATGTCAGGACAATCTGGAAGAAGAATT
GCAGAAACACATCGAGGAGAGCCAGGCACTGTCCAAGCAAAGCTGCGCTCTCTACCAGACCTTAGGAGAC
TACAAATTACAAAATCTGTTCCTTATTGGTTACACGAGGAAAGCCCCTCAGCTGACCTCAGCAGAGCTGA
TCGACCTCACCGGGAAGATGGTGAGCATTGCCTCCACGTGCTGCCAGCTCAGCGAGGAGAAATGGTCCGG
CTGTGGTGAGGGAATGGCCGACATTTTCATTGGACATTTGTGTATAAGGAATGAAGCAAGCCCTGTGAAC
TCTGGTATCAGCCACTGCTGCAACTCTTCGTATTCCAACAGGAGGCTATGCATCACCAGTTTTCTGAGGG
ATGAAACCTATGCCCCTCCCCCATTCTCTGAGGATAAATTCATCTTCCACAAGGATCTGTGCCAAGCTCA
GGGCAAAGCCCTACAGACCATGAAACAAGAGCTTCTCATTAACCTGGTGAAGCAAAAGCCTGAACTGACA
GAGGAGCAGCTGGCGGCTGTCACTGCAGATTTCTCGGGCCTTTTGGAGAAGTGCTGCAAAGCCCAGGACC
AGGAAGTCTGTTTCACAGAAGAGGGTCCAAAGTTGATTTCCAAAACTCGTGATGCTTTGGGCGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209348 protein sequence
Red=Cloning site Green=Tags(s)

MKWITPASLILLLHFAASKALHENEFGIASTLDSSQCVTEKNVLSIATITFTQFVPEATEEEVNKMTSDV
LAAMKKNSGDGCLESQLSVFLDEICHETELSNKYGLSGCCSQSGVERHQCLLARKKTAPASVPPFQFPEP
AESCKAHEENRAVFMNRFIYEVSRRNPFMYAPAILSLAAQYDKVVLACCKADNKEECFQTKRASIAKELR
EGSMLNEHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECCQGNSLECLQDGEKV
MTYICSQQNILSSKIAECCKLPMIQLGFCIIHAENGVKPEGLSLNPSQFLGDRNFAQFSSEEKIMFMASF
LHEYSRTHPNLPVSVILRIAKTYQEILEKCSQSGNLPGCQDNLEEELQKHIEESQALSKQSCALYQTLGD
YKLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASTCCQLSEEKWSGCGEGMADIFIGHLCIRNEASPVN
SGISHCCNSSYSNRRLCITSFLRDETYAPPPFSEDKFIFHKDLCQAQGKALQTMKQELLINLVKQKPELT
EEQLAAVTADFSGLLEKCCKAQDQEVCFTEEGPKLISKTRDALGV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007423
ORF Size 1815 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007423.4
RefSeq Size 2086 bp
RefSeq ORF 1818 bp
Locus ID 11576
UniProt ID P02772
Cytogenetics 5 44.77 cM
MW 67.3 kDa
Summary Binds estrogens, fatty acids and metals.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007423" in other vectors (4)
SKU Description Size Price
MC205574 Afp (untagged) - Mouse alpha fetoprotein (Afp), (10ug) 10 ug
$564.00
MG209348 Afp (tGFP-tagged) - Mouse alpha fetoprotein (Afp) 10 ug
$764.00
MR209348L3 Lenti ORF clone of Afp (Myc-DDK-tagged) - Mouse alpha fetoprotein (Afp) 10 ug
$864.00
MR209348L4 Lenti ORF clone of Afp (mGFP-tagged) - Mouse alpha fetoprotein (Afp) 10 ug
$864.00

Plasmid Map for MR209348

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209348
Circular map for MR209348

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